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Showing: 1 - 20 of 138 results

  1. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...to adapt the popular guide design tool Crispor by Max Haeussler. With a few extra lines of code, we enabled...protospacer + PAM) in the target region after HDR. If the max CFD score is less than 0.03, it stops mutating. (...Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement Andy May ...

  2. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...This post was contributed by Max W. Shen from MIT, Alvin Hsu Harvard University, and David R. Liu from... uncertainty. Many thanks to our guest bloggers Max W. Shen from MIT, Alvin Hsu Harvard University, and...from the Broad Institute and Harvard University. Max Shen is a Ph.D. candidate at MIT. His research uses...gradient descent in Pytorch, which gave us the maximum likelihood estimates of each parameter. We approximated...

  3. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ... researcher at the Friedrich Miescher Lab of the Max Planck Society. Zebrafish (Danio rerio) have been...Katherine Rogers from the Friedrich Miescher Lab of the Max Planck Society. Katherine W. Rogers is a postdoctoral...group at the FriedrichMiescher Laboratory of the Max Planck Society in Tübingen, Germany. She studies ...

  4. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...narrow excitation and emission peaks (max excitation at 493 nm and max emission at 509 nm), making it useful...GFPs and YFPs. Its excitation maxima is at 506 nm and its emission maxima is at 517 nm. Because mNeonGreen... to CFP484. Because the excitation and emission maxima were red-shifted (462 nm and 492 nm, respectively... mWasabi can be used with Sapphire (excitation maximum 399 nm) because it does not significantly excite...

  5. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...is organized by international PhD students at the Max Planck Institute for Biophysical Chemistry, Göttingen...a former Caltech researcher, now Director at the Max Planck Institute for Brain Research in Frankfurt ...

  7. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...contributed by Sana Khan Khilji, a PhD student at the Max Planck Institute of Colloids and Interfaces. Here...Germany. She will soon be joining her PhD lab at the Max Planck Institute of Colloids and Interfaces. Sana...

  9. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...store the solution at -20 °C. 1 mg/mL PEI-MAX Add 1 g of PEI-MAX powder to 900 mL deionized water in a 1... mg/mL PEI-MAX to the second tube of 6 mL BCD TFX (for a 1 mg/mL stock solution of PEI-MAX, 450 µg = 450...recombinant antibody plasmids using Polyethylenimine Max as a transfection reagent. After transfection and... Polyethylenimine hydrochloride, M.W. 40000 (PEI-MAX), Linear, Transfection Grade, VWR 75800-188 10% Pluronic...Before Starting Warm the DNA and working stock of PEI-MAX to room temperature before use, and warm the BalanCD...for 1 h in the 37 °C bead bath. Transfer the PEI-MAX and recombinant antibody plasmid DNA sample to the...ratios may range from 1:1 to 1:6. Add the diluted PEI-MAX to the diluted DNA. Cap the tube and vortex with ...

  13. An Addgene Summer Internship

    Type
    Blog Post
    ... safety reasons, and the insert size should be a max of 500 bp (preferably). I compiled a list of three...

  20. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGTTCCTGGTGGCGGTGCatgaattgggtcatgcaCTTGGTTTGGAGCACTCA max TAL3308 & TAL3309 TGAGCGACAACGATGAtatcgaggtcgacagtGATGTGAGTATAGTTA...
Showing: 1 - 20 of 138 results