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Showing: 1 - 17 of 17 results

  2. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGTTCCTGGTGGCGGTGCatgaattgggtcatgcaCTTGGTTTGGAGCACTCA max TAL3308 & TAL3309 TGAGCGACAACGATGAtatcgaggtcgacagtGATGTGAGTATAGTTA...

  3. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ...dCas9VP64 on pmax expression vector. 48224 pAC92-pmax-dCas9VP96 : dCas9VP96 on pmax expression vector. 48225... 48225 pAC93-pmax-dCas9VP160 : dCas9VP160 on pmax expression vector. 48226 pAC94-pmax-dCas9VP160-2A-puro...promoters. Table 2. pmax dCas9 Activator Expression Plasmids ID Plasmid 48223 pAC91-pmax-dCas9VP64 : dCas9VP64...Plasmid 48222 pAC90-pmax-DEST : Gateway Destination vector derived from Clontech's pmax (CAGGS) expression...activators and sgRNAs can be separately transfected (pmax dCas9 activators [48223~48227] + sgRNA expression...transferred to Gateway destination vectors (e.g., pmax-DEST [48222] and (Link opens in a new window) Lifetechnologies...2A-puro : dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Table 3. dCas9 Activators Gateway...

  4. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Mouse IgG2a 114541 Anti-KCNU1/Slo3 maxi-K+ channel [N2/16R] KCNU1/Slo3 maxi-K+ channel Mouse Mouse IgG2a 114542...IgG2a 128634 Anti-Slo1/BKAlpha maxi-K+ channel [L6/23R] Slo1/BKAlpha maxi-K+ channel Mouse Mouse IgG2a ...IgG2a 177486 Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R] Slo1/BKAlpha maxi-K+ channel Mouse Mouse IgG2a ...IgG2b 199406 Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R-2b] Slo1/BKAlpha maxi-K+ channel Rat Mouse IgG2b 199407...IgG2a 206523 Anti-Slo1/BKAlpha maxi-K+ channel [L6/48R] Slo1/BKAlpha maxi-K+ channel Mouse Mouse IgG2a ...IgG2a 222180 Slo1/BKAlpha maxi-K+ channel [L6/60R-rat IgG2a] Slo1/BKAlpha maxi-K+ channel Mouse Chimera...Mouse 199421 Slo1/BKAlpha maxi-K+ channel scFv [L6/60] L6/60 scFv Slo1/BKAlpha maxi-K+ channel Rat Mouse 199422...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... M, Rollins MF, Hidalgo-Reyes Y, Wiedenheft B, Maxwell KL, Davidson AR. 2015. Multiple mechanisms for ...136-9. PMID: 26416740 Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. 2013. Bacteriophage genes that...Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR. 2016. Naturally occurring off-...

  6. CRISPR Guide

    Type
    Collection
    ... a structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy between.... PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson, A. R. (2013). Bacteriophage...Rollins, M. F., Hidalgo-Reyes, Y., Wiedenheft, B., Maxwell, K. L., & Davidson, A. R. (2015). Multiple mechanisms...Kranzusch, P. J., Sontheimer, E. J., Davidson, A. R., Maxwell, K. L., & Doudna, J. A. (2017). A Broad-Spectrum..., J., Edraki, A., Shah, M., Sontheimer, E. J., Maxwell, K. L., & Davidson, A. R. (2016). Naturally occurring...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... 50% of its maximum value. Maturation is the time for fluorescence to obtain half-maximal value. Structure...Color) For each table: Excitation and Emission maximum are listed in nm. Brightness is the product of ...

  8. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...inhibit MMR PE5 - PE3 plus MLH1 mutant to inhibit MMR PEmax - optimized PE2 enzyme containing RT optimized for...RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA - additional...

  9. CRISPR References and Information

    Type
    Collection
    ...mulatta). Cas-Designer searches for targets that maximize knockout efficiency while having a a low probability...dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92 , pAC93...

  12. AAVED

    Type
    Collection
    ...perform, recommended histology conditions, minimum/maximum incubation time after in vivo delivery, etc.) Spark...

  14. Plan Your Experiment

    Type
    Collection
    ... it is also important to consider factors that maximize cleavage of the desired target sequence or on-target...

  15. Luciferase Plasmid Collection

    Type
    Collection
    ...self‐labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Conformationally...

  16. Cre-lox system

    Type
    Collection
    ...probabilistically ON or OFF following Cre recombination to maximize label diversity Mammalian Megason 13769 pCALNL-DsRed...
Showing: 1 - 17 of 17 results