...Candidate, University of Ibadan, Nigeriaunder supervision of Dr. Akindele Oluwatosin Adeyi, University ...Otito Izu is a PhD Candidate working under the supervision of Akindele Oluwatosin Adeyi at the University...the user.   At Addgene we're happy to be able to support diverse groups of scientists working in a wide ...

... 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of gene editing...Kim et al 2014) have shown that this technique supports extremely high frequencies of genome cutting and...nucleofection as (in our hands) this technique supports gene disruption or gene replacement frequencies...

...Gentamicin is often used as a supplement in mammalian cell culture to suppress bacterial growth, and is not...like gentamicin, neomycin is typically used to suppress bacterial growth. The selection agent should not...

...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression...Fishell   Optogenetics Optogenetics continues to supply neuroscience with ever more efficient tools for...

...adenovirus genes – E4, E2a, and VA – which are supplied on a helper plasmid and mediate efficient viral...including the ones listed previously). E3/E1B suppresses both apoptosis and the host immune response to...making it replication deficient. This is typically supplied by the production cell line (HEK-293) during production...

...openUC2, which I am a co-founder of), that aim to support the community in developing better, more sustainable...Challenges such as the provision of an accessible supply chain for parts that are listed in the bill of ...-based models and the use of taxpayer money to support this research. But there is a move towards more...

...aspects of my work but also found fulfilment in supporting my team’s development and career growth. This...check-ins, which helped them stay on track and feel supported. Another team member was highly self-motivated... served as a great "baby project," offering a supportive environment to build my management skills. Finally...

...Renilla luciferase variant fused to an FP; when supplied with a luciferase substrate, the luciferase transfers... a luciferase substrate like coelenterazine is supplied in the culture medium to allow RLuc8 to produce...coelenterazine-h to coelenterazine-h, producing a constant supply of substrate. Using this compound to excite the...

...save yourself a headache later on. Enhancer and suppressor screens These types of screens are very similar...identify manipulations which either enhance or suppress the previously defined disease phenotype. These... a mutant phenotype, very similar to enhancer/suppressor screens. Selections Selections differ from screens...

...COVID-19 pandemic, students will face a reduced supply of internships as a result of the necessary restrictions...the request to help your recommenders deliver a superb and detailed portrayal of yourself. Be mindful ...likelier you will end up with a stellar letter of support.        5. Application submission  Set a calendar...

...Nobel Prize in Chemistry for his contributions to super-resolution light microscopy, comes from his recent...Communications article “SRpHi ratiometric pH biosensors for super-resolution microscopy.” Endosomes are historically...studying endosomes, the Hell lab designed their super-resolution pH indicators (SRpHi) biosensors to meet...

...was particularly helpful for securing funding to support this effort. The future of reproducibility workshops...Science mini-grant, which will fund a project to support a dedicated website to host all the materials in...collaboration is that it is driven by academics, with support of industry partners, not the other way around....

...genes with 6 gRNAs per gene. The second is a "supplementary" library that contains an additional 6 gRNAs...expression of the MutL mutant enables transient suppression of DNA repair during oligonucleotide integration...Zurovec pBlue-TAL Nathan Lawson pJDS Series   Superglue proteins with SpyTag/SpyCatcher and SnoopTag/SnoopCatcher...connectors” that link tags to the targeted gene are supplied to the reaction from a prebuilt donor plasmid ...sequences for homology arms and the guide RNA are supplied as annealed synthetic oligos - eliminating the...

...of xanthine into guanine, and without xanthine supplied in the media, these bacteria are unable to divide...was able to grow this strain in minimal media supplemented with various caffeinated beverages, but not ...Angela Holmes Jin Zhang’s lab is developing new super-resolution techniques for live-cell imaging of the...

...Commun. 2016 Bo Huang spGFP Split superpositive GFP Split-superpositive GFP reassembly is a fast, efficient...Biotechniques. 2006 Chang-Deng Hu Green sfGFP Split super-folder GFP, our most-requested GFP(1-10) and GFP...

...enhancers and chromatin looping play important supporting roles. Enhancers are cis regulatory DNA sequences...promoters or genes each enhancer is interacting with. Super enhancers are particularly good models for building...simultaneously characterize the ‘social networks’ of 157 super enhancers.  3. Measuring temporal changes in chromatin...

... Association (PTA) has been instrumental in supplementing that curriculum. It’s through the PTA that I...Spangler Science The Fab Lab Middle school: supplementing the science curriculum Sharing my expertise ...you want to make sure their STEM education is supplemented. A 2009 study by the NSF showed that among US...

...mammalian cells) to collect the cell culture supernatant for further processing. It may be helpful to ...immobilized ligands are incubated with cell culture supernatant containing antibodies. The antibodies bind the...Rockberg J, Chotteau V. Small-scale bioreactor supports high density HEK293 cell perfusion culture for...

...binding) Google Maps: Enhancers and genome math Suppose that you wish to use Google maps to find all coffee...disrupt these enhancers. A real-world experiment Suppose you run a ChIP-seq experiment (think “GPS for proteins...we all know and love. To understand compaction, suppose you have a piece string that’s 10 meters long. ...

...but you have definitely reaped the benefits. We support everyone that works with Addgene in some way. For...the development process at Addgene. Diversity supports our innovation Our software development team is...This can lead to confusion as to what the code is supposed to be doing. When this happens, we discuss and...