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Showing: 81 - 100 of 184 results
  1. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...the MUCICAT system has the following advantages: Fast and site-specific multicopy chromosomal integration... The pEcCas plasmid can be operated at 37 ℃ for faster growth of its E. coli host, and the addition of...industrial strain. Not only is MUCICAT better, it is faster. Using MUCICAT only needs 2 newly constructed plasmids...on developing microbial genome editing tools for faster construction or reprogramming of cells to understand...
  2. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...having high fidelity during action potentials of fast-spiking neurons. The same A-to-D mutation in the...
  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ... vitro first. In vitro screening is a simple and fast method for validating your selected gRNA’s, and ...ready for purchase. But what is the easiest and fastest way to use the system in mice? We'll have a post...
  4. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... the most studied prokaryotic organism. With its fast growth rate, small size, and low cost, it’s understandable...
  5. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...challenging samples as in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK... 100% negative prediction rate. It is also the fastest test that has been developed so far.  A similar...
  6. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...limitations (Figure 1). Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of... below use the following optogenetic modules: VVDfast homodimerizes under blue light iLID-SspB heterodimerizes...of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and SspB-mCherry-p60 (katanin) are... of two separate blue light-sensitive systems, VVDfast to control recruitment of an “anchor” to microtubules...anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module and the SspB-mCherry-p60 katanin...controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and other optogenetic modules...
  7. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...varies for different enzymes. Many companies now sell fast digest enzymes that can digest large amounts of ...
  8. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... find today are marketed for a specific purpose: fast growth, high-throughput cloning, routine cloning...
  9. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... later became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR...ligands (Wilhelm et al., 2021). While HaloTag reacts faster for some dyes, others, especially some nonfluorescent...
Showing: 81 - 100 of 184 results