...through normal mechanisms. It will take the full breadth of our research expertise, but we believe that ...health for this current COVID-19 pandemic, and outbreaks that have yet to come,” said the team from the...

...searching the term "breast cancer" returns 40 screens performed in 26 different breast cancer cell lines...

...that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...cytoplasmic retention insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation...luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation of varying...principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla luciferase...

...stranded DNA at this sequence. The energy from this breakage is stored as a covalent bond between the cleaved... of TOPO cloning happens. Basic procedure Let’s break down the steps needed for TOPO cloning: 1. Create...

...research studies highlight the importance of taking a break and incorporating EAs into your routine.  Getting...learn new skills, while also getting a much-needed break from your lab bench.  Want further information ...

... during the day is a great way to take a mental break, while meditating right before bed will help you...it. Plan defined work hours as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique...

... biosensor with two components - an enzyme that breaks down the HMO of interest into its component simple...and a lac operon controlled GFP. When the enzyme breaks down the HMO of interest, the lactose released ...

...roles, including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist...

...doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field. It has the possibility...7). An independent team led by Hans Clevers at Hubrecht Institute in The Netherlands used the CRISPR/Cas9...

...it’s acceptable if it’s in the name of beer and bread. The following table lists some of the biological...end joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination (HR)....

...important biological information, amino acid abbreviations, and structures into one convenient resource...say that I still have the single and triple abbreviations of the amino acids memorized, but the structures...

... Cleave target DNA resulting in a double-strand break (DSB) Cas9 and its variants have two endonuclease... which is E) cleaved leaving a F) double-strand break. A “gRNA” is a researcher-designed hybrid of the...

...principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase variant...exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient signal for in vivo and in...

...unedited site distal tothe predicted double strand break, and a NHEJ/ “drop-off” probe designed to bind directly... directly at the predicted double strand break site (Figure 2A). The single molecule resolution of digital...

...elicit downstream signaling cascades.  So let’s break down the name "G-protein coupled receptor" into ...via bioluminescence resonance energy transfer 2 (BRET2). Check out our blog post on GPCR signaling using...

...and ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant housing...

...upregulation is correlated with poor survival in breast cancer patients. By digging into the literature...containing RNAs could play a role in the severity of breast cancer. Infectious Disease. RaPID can also help...

...untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene...enhances endonuclease delivery for double-strand break repair." Gene therapy 17.9 (2010): 1175-1180. PubMed...

... else to read and / or give yourself a bit of a break before you read over it again with fresh eyes and...your institute or a private funding body. Success breeds success and I have certainly found that the experience...

...incubating Nanoblade particles with polybrene, a cationic polymer. Polybrene has long been used to increase...