...mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1-expressing mouse neuron without...optogenetic silencer in experiments with mouse cortical neurons, far out-performing previous attempts to engineer...of HcKCR1-EYFP and tdTomato expressed layer 2/3 neurons in mouse. C) Action potential frequencies with ...channels that mediate optogenetic inhibition. Nat Neurosci 25, 967–974 (2022). https://doi.org/10.1038/s41593... of Addgene's more than 50 antibodies target neuroscience-related proteins, but we recently added several...

...of Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j.neuron.2016.04.030    Bradbury ... to the catalog. The Catalog Will Start With Neuroscience Antibodies and Grow From There Addgene’s antibody...Brain Research Through Advancing Innovative Neurotechnologies® (BRAIN) Initiative. Our first collection ...materials is being developed for scientists studying neuroscience and is available to both academic and industry...Addgene will continue to add more antibodies, for neuroscience research and other disciplines, over time. The...

...findings relevant to my background (neuroimaging, biomechanics, neuropsychology) and from areas in which I knew...sentiment). Entering the final semester of a neuroscience major, I was struck with the realisation that...that I didn’t want a career in neuroscience—whatever that entails. Despite being engrossed by the theory...spheres of research. In my case, an interest in neuroscience was carried over and allowed to percolate within... notion of “heading” in soccer as a possible neurological health risk. Having played the sport my entire...

...second vector that delivers gRNA, such as lentiGuide-Puro. The alternative single-vector systems typically...signaling pathways in specific cell types (typically neurons). While all of these viruses are popular, pAAV-...pAAV-hSyn-DIO-hM4D(Gi)-mCherry can be used to induce neuronal silencing in vitro and in vivo (Krashes et al..... The human synapsin promoter further specifies neuron-specific expression of the DREADD (Kügler et al...Lowell BB. Rapid, reversible activation of AgRP neurons drives feeding behavior in mice. J Clin Invest..... Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an...

... split botulinum neurotoxin halves to enable VAMP2 cleavage, and suppresses neuronal transmission within...core of this tool is the endoprotease Botulinum Neurotoxin B (BoNT/B), which cleaves the synaptic protein...VAMP2 renders it inactive, thus inhibiting neurotransmitter release. How is this light-mediated? The researchers...photodimerizer system CRY2-CIB1 to regulate botulinum neurotoxin were also created. The depositors demonstrated...split BoTN/B plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed PMID: 30704911. Acoustic reporter...

...stimulation is a powerful tool used by neuroscientists to interrogate neuronal networks. A major drawback of this... method is the stray excitation of neighboring neurons in opsin dense regions. To overcome this issue,... C-terminus of the protein. Unlike the diffuse neuronal expression observed with wild type CoChR, soCoChR...describe a novel system for the precise mapping of neuronal networks at the single-cell level. Various soCoChR...available at Addgene. Shemesh OA, et. al. Nature Neuroscience. 2017. PubMed PMID: 29184208.        Synthetic...

... RNA-sequencing to identify neuronal class and deep sequencing of neurons from each class to fully characterize...Addgene.  C. elegans open resources The C. elegans Neuronal Gene Expression Map & Network (CeNGEN) The CeNGEN...comprehensive gene expression atlas of every C.elegans  neuron. Scientists are currently doing this using two ...to view the anatomy of worm layer by layer, the NeuroML C. elegans connectome, a model of the C.elegans...Gene Expression Map of an Entire Nervous System." Neuron 99.3 (2018): 430-433. PubMed PMID: 30092212. PubMed...

...across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by action potentials ...perceived signals to physical actions. Understanding neuronal pathways and the function of certain cells depends...be directed to specific types of cells, such as neuron subtypes, thanks to viral serotype specificity ...available VSDs has been successful even at the single neuron level in vitro. In vivo monitoring has also been...voltage is particularly physiologically relevant in neurons, myocytes, and endocrine cells. It follows that...voltage indicators advance their applications in neuroscience. Curr Opin Biomed Eng. 2019. 12:111-117. doi...

...Lee and Pantelis Tsoulfas of the Department of Neurological Surgery at the University of Miami. The beginning...light microscopy, particularly related to the neurosciences.  These developments in microscopy coupled with...of transgenic mice in which a subpopulation of neurons are brightly labeled with GFP (e.g. Thy1-YFP-H ...LSFM with multiple tissue clearing strategies and neuronal labeling methods will greatly aid our understanding...331–336. Pubmed. P. Keller, M.B. Ahrens.  (2015) Neuron, 85, 462-483. Pubmed. E.H.K. Stelzer. (2015). Nature...H. Lee, A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2) e0001-15.2015. Pubmed. ...

...could identify the ancestry of the cell lines (European, East Asian, Indigenous American, Africa, or South...lines used in DepMap CRISPR screens are of either European or East Asian ancestry, Sean and Aaron knew that...dependencies that were clearly associated with European or East Asian ancestry groups, far more than expected...human population. Rather, it primarily reflects European ancestry.  Understanding the impact Fig...to better understand how the use of a primarily European reference genome could affect their analysis. ... by the Cancer Dependency Map) than lines from European or East Asian ancestries. These SNPs, however,...

...unable to reach all regions of the CNS. PHP.B, a neurotropic variant of AAV9, is better than AAV9 at gaining...receptor used by PHP.B viruses. Creating PHP.B, a neurotropic AAV9 variant PHP.B’s specificity for C57BL/6J... and transduces the majority of astrocytes and neurons across multiple CNS regions. PHP.eB, an enhanced...PMID: 30819613. Hordeaux, Juliette, et al. "The neurotropic properties of AAV-PHP. B are limited to C57BL...transduction efficiency in the marmoset brain." Neuroscience letters 665 (2018): 182-188. PubMed PMID: 29175632...central and peripheral nervous systems." Nature neuroscience 20.8 (2017): 1172. PubMed PMID: 28671695. PubMed...

...various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The ...catalog is a new class of sensors for neuropeptides and neurotransmitters. Motivated to develop a biosensor...MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved sensors...

...When neuroscience graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of...now available in Addgene’s repository for use in neurons, both in culture and in living brain tissue. “The... the LITEs to control gene expression in mouse neurons and in the brains of living mice both quickly and...clear application to anyone with an interest in neuroscience, their use is by no means limited to particular... want to apply LITEs for the transformation of neurons, she offers a tip: save yourself some time by making...

...Access to Projection Neurons. Neuron, 92(2), 372–382. https://doi.org/10.1016/j.neuron.2016.09.021 Walters..., 2023). It may be a good choice for targeting neurons, with high transduction frequencies in the central...to target the central nervous system, including neurons, AAV5 is generally the best choice for targeting... is derived from AAV2 and engineered to infect neurons and undergo retrograde transport, traveling up ...

...for neuronal systems. To develop a new single-component tool for magnetogenetic control of neurons, the...pTALYM4 Boris Greber pTAL7a, pTAL7b Michal Zurovec pBlue-TAL Nathan Lawson pJDS Series   Superglue...McMahon et al., report using as few as 150 fly neurons as their starting material! Thus, TRIBE can be ...non-invasive and can rapidly and reversibly stimulate neurons. Recent studies have designed multi-component actuators...finding that it could increase the activity of neurons from brain slice preparations. Magneto2.0 was also...Magneto 2.0 allows for magnetic remote control of neuronal activation. Find Magneto 2.0 plasmids by following...following the links below. Wheeler M, et al. Nat. Neurosci 2016. PubMed PMID: 26950006 Plasmid ID Plasmid...

...Detection of Norepinephrine. Neuron, 102(4), 745-761.e8. https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ... B, Representative images of HEK293T cells and neurons expressing nLightG or nLightR before/after application...dashed lines. Scale bars, 10 μm (HEK293T), 20 μm (neurons). Figure and caption adapted from Kagiampaki et...

...detect the neurotransmitters and other bioactive molecules is integral to the study of neuroscience. To this...improved detection for tracking large populations of neurons in wide-field imaging (Dana et al., 2018). See ...chemogenetic AAV for activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab...with Cre for intersectional control of specific neuronal populations. We now offer a Flp-expressing AAV...

... Nature Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969-4.   Increased neuronal expression...flexibility for in vivo proximity labeling Increased neuronal expression using Cre-mediated AAV Targeted Evolution...found at least two variants that display increased neuronal expression in the brain while having decreased...M-CREATE plasmids here!  Goertsen D, et al. Nature Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969...

...29] Photoreceptors [30] Otic hair cells [31] Neurons [32, 33, 34] You can find a variety of plasmids...have been successfully transdifferentiated into neurons and β-cells [40-42]. Reprogramming and transdifferentiation...S.C. Zhang, Directed differentiation of dopamine neurons from human pluripotent stem cells. Methods Mol ...human pluripotent stem cells to cerebral cortex neurons and neural networks. Nat Protoc, 2012. 7(10): p...pluripotent stem cells to mature functional Purkinje neurons. Sci Rep, 2015. 5: p. 9232. PubMed PMID: 25782665...Direct conversion of fibroblasts to functional neurons by defined factors. Nature, 2010. 463(7284): p....

...the data is heavily dominated by the U.S. (42%), Europe (23%), and China (14%). Spikes in plasmid requests...majority of our plasmid orders come from the U.S. and Europe, we see a corresponding dip in plasmid request ...following hierarchical model: Because the U.S., Europe, and China account for over 75% of all of our plasmid...(the last date in our dataset) for the U.S. and Europe. To improve the stability of our model, we considered...scientists placing orders per day across regions, with Europe most impacted and China least impacted. The inferred...