...enzymatic activity is measured. The effect of promoters or enhancer regions on gene expression can be ...investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect...are cloned into a plasmid upstream of a minimal promoter linked to luciferase. Activation of the pathway...

... expressed under promoters like the tet-responsive element (TRE) or the cFos promoter. For example, neurons...inducibly express tagged iPAK4 from different promoters, specific time points appear as fluorescent bands...new way to record and quantify the dynamics of promoter activity in single cells. Find iPAK4 plasmids ...

...guide RNA disrupting mutations in donor DNA   Promoting HDR events over other edits Like I said earlier...transition out of S phase, so cells stay in an HDR-promoted cycle longer. This approach may have some negative...eLife)! Use a Cas that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It...generates sticky ends upon cutting. Cas12a may promote HDR by generating ssDNA overhangs at the break ...

... may differ (e.g., a set of various mammalian promoters), the same cloning procedure can be used to incorporate... in this method include fluorescent proteins, promoters, enhancers, polyadenylation signals, inducible...Table 1). Each construct was flanked with a CMV promoter (to drive high-level expression) and a polyA signal...multiple identical polyadenylation signals or promoters Gibson et al., 2009 MXS-Chaining, BioBricks,...

...career in science outreach: engaging the public to promote an awareness and understanding of science. Like...spray paint to make a trophy for the winner. We promoted the contest for about two months, and we got one...social media. I gained a concrete example to use to promote future attempts and learned a bit about recruiting...network and graduate school resources daily to promote CINSO events and recruit speakers and participants...

...you design your donor template to include a T7 promoter upstream of the left homology arm, perform in ...transcription to create your ssDNA. Note that the T7 promoter will not be included in the final ssDNA template...GG); this design has been shown to increase T7 promoter activity. To avoid re-cutting by Cas9 after editing...containing the homology arms on both sides and the T7 promoter on one end. Typically, Easi-CRISPR works best ...Addgene plasmids. Design PCR primers to add T7 promoter-left homology arm and right homology arm to the...

...induce transcription at CpG regions of various promoters. Other researchers additionally fused a TAL effector...: dCas9-p300 adds H3K27ac marks to histones in promoter and enhancer regions. These marks are associated...Figure 2: dCas9-Tet1 demethylates cytosines at promoters and enhancers. This targeted demethylation is ...Figure 3: dCas9-DNMT3A methylates cytosines at promoters and enhancers. This targeted methylation is associated...-based acetyltransferase activates genes from promoters and enhancers. Nature Biotechnology 33:510–517...

...gene between two inverted T7 promoters. Transcription from each promoter progresses past the specific ...T444T, contains T7 terminators adjacent to the T7 promoters to prevent the incorporation of non-specific nucleotides...

...mCherry, all under the control of the human synapsin promoter. Purpose of this AAV pAAV-hSyn-DIO-hM4D(Gi)-mCherry... to a particular cell type. The human synapsin promoter further specifies neuron-specific expression of...Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene...

...400-500 bp cassettes containing the RNA pol III promoter, gRNA and pol III terminator. These relatively...together in a single vector. In addition, the pol III promoter is relatively weak, and low expression of gRNAs...biological systems. Size is also a factor; the pol lll promoter and terminator need be encoded only once, and ...

...examined a number of important plasmid elements – promoters, origins of replication, protein tags, and antibiotic...Regulated Cre expression – placing Cre downstream of promoters that are active only in certain cell or tissue...Cre under a variety of ubiquitous and regulated promoters, and many loxP-containing alleles have also been...

...optimism they encapsulate, and the solutions they promote help motivate you to spend a little time advocating...fight is far from over here at home.  The work of promoting and defending the role of science in our democracies... take in abusing climate science skepticism to promote harmful policies that benefit polluting industries.In...marching in and of itself will be enough. IT WON’T! Promoting the incorporation of science in policy, demanding... that uses yeast cells to study how DNA damage promotes cancer. She is also a science communicator, UBC...

...-inducible promoter, though Khetrapal et al. also demonstrated that the arabinose promoter worked too.... under both rhamnose- and arabinose-inducible promoters. Tight control of toxin expression is absolutely...a rhamnose-based tightly regulated and tunable promoter system." Biotechniques 40.3 (2006): 355-364. PubMed...

...repeat (LTR) of the HIV-1 genome, which acts as a promoter, and found that SAM targeting of the enhancer ...NF-KB binding sites increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent...cells with activated HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell lines that can ...indels may not ablate viral function, but rather promote resistance to CRISPR/Cas9. While these results ...

...published systematic reviews on an agent used to promote bone growth based on data voluntarily made available...collaborative endeavor guided by aspirations to promote open science, ensure good stewardship of clinical...

...resources and information about organizations promoting reproducibility in this booklet (which was also...line we may see further adoption of policies that promote reproducible publishing practices. One example ... also hopefully see a shift toward hiring and promotion practices that focus on reproducibility and ensuring... Including descriptions of many organizations promoting reproducibility List of Tools for Reproducibility...

...and are easily adaptable for use in your lab! Promoting personal development through thoughtful planning...love to hear about the steps taken in your lab to promote personal development - let us know your strategies...

... cells where common eukaryotic promoters (such as CMV or EF1A promoters found in many CRISPR plasmids)...transfection or viral transduction     Appropriate promoters must be chosen for both Cas9 and gRNA expression...

... site accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the homology... a direct fusion of the desired gRNA to the U6 promoter, and a second plasmid containing the homology ...regions of homology to the gene of interest to promote homology directed repair after a CRISPR/Cas9 induced...

... targeting expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of...channel, such as the serotonin 3 receptor, will promote depolarization in neurons. Alternatively, combining...anion channel, such as the glycine receptor, will promote hyperpolarization. PSAM ligands The advantage of...