...University studying mammalian immunology, and in his free time, he is pursuing a plant engineering project... cell culture and transgenesis he wants to share his knowledge with the community by teaching a DIY plant... entrepreneurs using our space to perform early-stage experiments. For example, Dave Grawoig is using ...

... for the CZ Biohub project by Manuel Leonetti to tag all 22,000 protein coding genes in the human genome... at the Biohub in San Francisco. Before starting his career in software, he completed a MSc in Psychology... tools which were optimized for HDR, except for TagIn. HDR requires the additional step of mutating guide...cut-to-insert distance for HDR. (The notable exception is TagIn––see their "summarisation score".) For CrispyCrunch...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide...

...-28b-Cas9-His 6xHis (C terminal); NLS (C terminal) 62933 pET-Cas9-NLS-6xHis 6xHis (C terminal...Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N terminal); 6xHis (N terminal); 2xNLS (C terminal... be selected, since it cleaves at the stop codon TAG. Design your repair template with 55-100 base...; NLS (C terminal) 62934 pET-NLS-Cas9-6xHis 6xHis (C terminal); NLS (N terminal)   Easi-CRISPR...

...’re attending SfN2019, stop by booth #2163 to say hi and learn more about Addgene’s viral service. Additional...topic as shown in the graphic below. Follow the hashtag #AddgeneGoesViral to find information on the basics...

...expression of the HIS3 gene, which is required for histidine manufacture and therefore growth on his-deficient...Post About Yeast Vectors Read our Blog Post About Tagging Yeast Genes ...activator (1a) or a repressor (1b). Scientists took advantage of these discoveries to create a new system for...that repress instead of activate transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait... is an example of the former. In this system, histidine-auxotrophic host strains are used to select for...subset based on a cellular compartment or growth stage). A prey library can either be applied to the bait...

...promoters, terminators, solubility and purification tags, connectors, cDNAs, and more to find the perfect...constitutive and inducible promoters, linkers, and tags makes EcoFlex a great option for those interested... a larger library of parts but don’t need to add tags to your proteins, then the CIDAR Kit is an excellent...Stroik and Meghan Rego, with thanks to Rob Hurt for his expertise and input.  References and resources References...

...Neuroscience Conference 2023? So are we! Come say hi to us at Addgene's booth, #3422, in the nonprofits...also be sharing our conference experience on X, Instagram, and Bluesky.   Finally, if you're looking to ...

...resources. Listen to hear all about Professor Goedhart, his lab, and some of the tools he’s developed.   Listen...creation of improved fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent...

...guest blogger, Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...Research Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory... be needed to achieve your intended next career stage and create a timeline for achieving these goals....publications (published, not in preparation) A history of successful funding (fellowship grants) A project...

... in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...rAbs are a relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression...

...supervisor’s management style, bring challenges to his or her attention as soon as you can. It is in your...you communicate your ideas assertively, listen to his or her viewpoint, and discuss options for mutually...people: Hostile-aggressives, who are usually antagonistic and impolite, Complainers, who spend most ...

...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely ...different epitopes. Detection of overexpressed (tagged or untagged) protein When expression plasmids are available...population (pink histogram) compared to the WT cells (green histogram). The unfilled histograms show cells ...400,000 cells were stained with the indicated Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite...You can see an example of their work on their synaptotagmin 1 page. The data produced is available on the...interpretation of your results. Therefore, taking advantage of antibody validation efforts and information...to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot...

...innocuous tags” (inntags) less likely to alter a protein’s properties. Why do we need new epitope tags? Short... looking to a tag a protein, inntags IT5 and IT6 are a great place to start. These tags are easy to use... about other Protein Tags in our Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins ...major caveat - a tag may interfere with protein localization and/or function, so each tagged protein must ...proteins found that 20% of tagged proteins did not localize similarly to their untagged counterparts. These ...the effects of GFP fusions to inntags or traditional FLAG, HA, and Myc tags. Impressively, IT6-GFP did not...of the traditional tags were suitable for both applications. To prove that inntags are indeed innocuous...

..., modular plasmids, combining fluorescent tags, epitope tags, protease sites, and selection markers. These...fluorophore tagging vectors for your single or multi-color imaging experiments. Interested in epitope tags? Others...have genome tagging systems enabled you to advance your research? Do you have a favorite tagging system that...epitope tag (12xHis, 2xStrep, 3xFlag, Protein A, or V5), and selection marker (KanMX, HphMX, or His3MX).  ...Researchers have long taken advantage of this natural process to integrate protein tags into the genomes of S...These tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5, or...especially useful in protein complex studies where tagging of multiple protein products is desired, as multiple...

...Cruz where his group focuses on gene regulation in nematode molting and spermatogenesis. His group also...conditionally manipulate proteins. Learn more at his webpage or follow him on Twitter.   References Arribere...-CRISPR and co-conversion approaches have the advantage of being used in any genetic background, but require...

...Matthijs Dorst’s Deck the Lab Nativity scene below his Christmas tree! It is that time of the year again...showcase it all!           View this post on Instagram                       A post shared...dyed with real microbial pigment. Check out the hashtag to see the description of all the ornaments. #DeckTheLab...

...University. Max Shen is a Ph.D. candidate at MIT. His research uses applied machine learning and statistical...synthesis are four examples of technologies pioneered in his laboratory. Learn more here. Additional resources...effect on plasmid requests Before we began any sophisticated analyses, we first visualized our data in various... 5: Inferred effects. Values are the inferred percentage of normal activity (the average rate of unique...

...in Molecular Plant Sciences since December 2020. His research focuses on the efficiency of transposon-... with low efficiency.       Sheng Yang obtained his Ph.D. from Shanghai Institute of Biochemistry, Chinese...the newly generated pEcCas/pEcgRNA system.   Advantages of the pEcCas/pEcgRNA system Compared with the...conclusion, the MUCICAT system has the following advantages: Fast and site-specific multicopy chromosomal...

...colocalization of GFP tagged HA frankenbody with an mCherry, 4xHA tagged histone protein (H2B) in U2OS...could also bind a 1xHA tagged histone protein as well as a mitochondrial protein tagged with spaghetti monster...other fluorescent proteins and tags including mCherry, HaloTag and SNAP-tag for multi-color imaging. Find... the 10xHA tag increased the fluorescent signal to noise ratio five fold over the 1xHA tag.  Lastly, the...use GFP to image nascent proteins. In addition GFP tags are large, dim, and permanently attached to a protein...limitations, scientists have co-opted antibodies for tagging purposes. Different antibodies bind different protein... called epitopes and scientists can use these to tag their proteins of interest. Antibody based probes...

...minorities to study STEM subjects. Here, Sadangi and his team answer a few questions about The Addictive Brain...sessions led by scientists, which is hosted on our Instagram channel every Sunday. What's planned for the upcoming...participate in our one-hour Live Q&A session on Instagram every Sunday. Undergraduate and graduate students...background. Social media is a powerful tool. Use hashtags effectively. This would help you direct your content...