...N-terminal 6x His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 Tac (lactose...pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally His-tagged TALEN...-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally His-tagged TALEN fused...artifacts!), photoactivatable tags for super resolution imaging, epitope tags like His for easy western blotting...recombinant protein with an N-terminal 6x His tag and an MBP tag. Contains a signal sequence to localize...different combinations of His and Strep tags as well as N-terminal PelB tags for localization to the periplasm...and C-terminal Strep, His, MBP, TRX, GST, SUMO, MISTIC, and LSL tags as well as tags for protein localization...

...Prey Cd4d3+4-COMP-b-lac His-tag Cd4d3+4-6his Bio-linker-his Cd4d3-bio-linker-6His* FLAG prey CD200-Cd4d3...COMP-blac-3xFLAG-6his Cd4d3+4-pentamer BasiginCd4d3+4-COMP-his-stop Type II expression CD69-His-bio Multi TM...as both monomeric baits and multimerised enzyme-tagged preys. Ectodomain inserts: The inserts that encode...expression vectors which have different C-terminal tags. Most vectors are based on the pTT3 plasmid backbone... TM expression CD36-bio-His Plasmid collections exist for: Basic AVEXIS : A list of all you need to get...Martin S, Söllner C, Charoensawan V, Adryan B, Thisse B, Thisse C, Teichmann S and Wright GJ Molecular & cellular...receptors Human erythrocyte Babesia microti blood stage proteins P. falciparum sporozoite cell surface and...

...mammalian expression 6xHis Epitope Tag pEZYmyc-His - C-terminal Myc-His tag for mammalian expression... His tagged bacterial expression vectors pET His6 TEV - N-terminal His6-TEV tag for ... araBAD p15TV-L - His-tagged bacterial expression vector (see tagged expression vectors...C-terminal 3xHA tag for yeast expression Myc Epitope Tag pKMyc - N-terminal Myc tag for mammalian...series - Expression with Strep, His or optimized YFP tags for purification or localization...promoter pCS2 mt-GFP - Create myc-tag, GFP-tag fusions for transcription pCSDest - Easy cloning...Gateway recombination Back to Top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools...

...optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian...optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian...Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian optimized... suitable for creating individual fluorescently tagged proteins to study protein-protein interactions ...

... (CAPTURE.) Tag : Multiple methods make it easier to tag endogenous loci with epitope tags or fluorescent...include mutations seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed... not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also be used to purify a genomic locus... EM, Myers RM. 2015. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res . ... CRISPR CRISPR History CRISPR History and Development for Genome Engineering ...

...Trono 14666 pQE9-His-p97(wt) VCP His T5 ALS Graham Warren 14667 pQE9-His-p97(QQ) VCP His T5 ALS Graham Warren...17229 pQE9-His-p97deltaD2 VCP His T5 ALS Hemmo Meyer 17230 pQE9-His-p97deltaD2(K251A) VCP His T5 ALS Hemmo...0005 VCP His T7 ALS Axel Brunger 12339 pBD-0071 VCP His T7 ALS Axel Brunger 12344 pBD-0010 VCP His T7 ALS...0016 VCP His T7 ALS Axel Brunger 12346 pBD-0013 VCP His T7 ALS Axel Brunger 12347 pBD-0006 VCP His T7 ALS...0009 VCP His T7 ALS Axel Brunger 12349 pBD-0070 VCP His T7 ALS Axel Brunger 12350 pBD-0069 VCP His T7 ALS...0008 VCP His T7 ALS Axel Brunger 12352 pBD-0007 VCP His T7 ALS Axel Brunger 12353 pBD-0018 VCP His T7 ALS...0020 VCP His T7 ALS Axel Brunger 12355 pBD-0022 VCP His T7 ALS Axel Brunger 12356 pBD-0024 VCP His T7 ALS...

... cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of...inexpensive to assay, and easy to purify using histidine-tagged constructs and routine protein and molecular...map, a TEV cleavage site is present between the 6xHis-tag and the coding region of MDH. For detailed clone...

...-His 52067 GSN-bio-His 52068 NID1-bio-His 52069 P4HB-bio-His 52070 PDIA3-bio-His 52071 PDIA4-bio-His 52072...TTR-bio-His 52175 AHSG-bio-His 52176 ALB-bio-His 52177 APOH-bio-His 52178 C12-bio-His 52179 F2-bio-His 52180...ITGA2B-bio-His 53356 OTOA-bio-His 53358 ENTP-bio-His 53359 P2RX1-bio-His 53360 P2RX6-bio-His 53361 ECE1-His-bio...bio-His 53426 F5-bio-His 53427 PLG-bio-His 53428 PLUNC-bio-His 53429 VTN-bio-His 53430 WNT11-bio-His 61486...ACVR1-bio-His 51624 PECAM1-bio-His 51639 SELP-bio-His 51640 ACVR1B-bio-His 51641 ADAM10-bio-His 51642 APLP2...APLP2-bio-His 51643 APP-bio-His 51644 G6B-bio-His 51645 GP5-bio-His 51646 CD226-bio-His 51647 CD34-bio-His...bio-His 51648 CD40-bio-His 51649 CD84-bio-His 51651 DAG1-bio-His 51749 EFNB1-bio-His 51750 EPHB1-bio-His...

...RAMA-bio-His 50800 Prohibitin-bio-His 50801 PF10_0166-bio-His 50802 GLURP-bio-His 50803 MSP3.5-bio-His 50804...MSP3.6-bio-His 50805 MSRP5-bio-His 50806 MSRP4-bio-His 50807 MSP8-bio-His 50808 PF13_0125-bio-His 50809 PF14...0044-bio-His 50810 PFA0135w-bio-His 50811 PFA0445w-bio-His 50812 SERA1-bio-His 50814 SERA2-bio-His 50815 ...SERA3-bio-His 50816 SERA4-bio-His 50817 SERA5-bio-His 50818 SERA6-bio-His 50819 SERA7-bio-His 50820 SERA9...-bio-His 50821 PFA0210c-bio-His 50822 PFB0475c-bio-His 50823 PFD1130w-bio-His 50824 RIPR-bio-His... invasion of the human erythrocyte by the blood stage of the malaria parasite, P. falciparum . Please ...

...Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene...empty plasmid backbones with different fluorescent tags for you to create fusion proteins with your gene...Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian Expression...dimerization pCEP4YPet-MAMM - Mammalian Expression pYPet-His - Bacterial Expression YPet-N1 - Mammalian Expression...Monomer pmiRFP670-N1 - Mammalian Expression pBAD/His-miRFP670 - Bacterial Expression iRFP670 643 670 13...Monomer pmiRFP703-N1 - Mammalian Expression pBAD/His-miRFP703 - Bacterial Expression iRFP702 673 702 8...

...Protein C, DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please visit our Tags and Other Markers Antibody... Glypican Antibodies Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids Welcome to the Institute...proteins (glypicans and integrins) and common epitope tags, as well as antibody plasmids to create or express...Reactivity Recommended Applications Browse Epitope Tag Antibodies IPI has developed recombinant versions...versions of antibodies that recognize common epitope tags to help purify, detect, or localize particular proteins...s full catalog of antibodies that target epitope tags and other common cellular proteins. ID Recombinant...

...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2...F391W-WPRE-SV40 Syn CaMPARI2-F391W his Constitutive 1 Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...L398T-WPRE-SV40 Syn CaMPARI2-L398T his Constitutive 1 Schreiter Calcium Sensor: jRGECO1 100852 pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40...Sensors GRAB_NE nLight Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ATP Sensors iATPSnFR2...-ChrimsonR-ST Syn jGCaMP8s none Cre-dependent 9 Histed 176749 AAV-Syn-H2B-jGCaMP8s-WPRE Syn jGCaMP8s none...GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2...KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...

...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4704 101064-AAV1 pAAV_hsyn_NES-his-CaMPARI2... Schreiter AV-1-PV4705 101061-AAV1 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 Biosensor Eric Schreiter...James M. Wilson AV-9-PV2453 45185-AAV9 AAV-EF1a-BbTagBY Control Joshua Sanes AV-9-PV2454 45186-AAV9 AAV-EF1a-BbChT...

...tRNA ( 68307 ) at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein...68295 GFP E17TAG Plasmid 68296 GFP S2TAG Plasmid 68297 GFP Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid...Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO MBP-MEK1 XX...68301 MBP-MEK1 S218TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68305 Beta lactamase S68TAG Plasmid 69118...-EFSep) Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 68283 SepOTSβ Plasmid 68284 SepOTSγ...69118 E17TAG GFP zeo resistance Strain 34929 BL21ΔserB Strain 52055 EcAR7 Plasmid 112031 pNAS1b split mCherry...

...Mammalian Sato 123128 pET-His-Cre 6xHis-Cre T7 Bacterial Liu 123131 pET-His-VCre 6xHis-VCre T7 Bacterial Liu...hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR Cre, KASH-tagged EGFP, and sgRNA expression hSyn AAV Zhang 60820...9xGGS-Cre-6xHis Cre fused to highly cationic GFP variant T7 Bacterial Liu 62939 pET-Cre-6xHis Cre-6xHis T7 Bacterial...73472 RabV CVS-N2c(deltaG)-mCherry-P2A-Cre Cre CMV RABV Jessell 73474 RabV CVS-N2c(deltaG)-Cre Cre CMV RABV...Simpson 106368 pCMV-Tag2B-NCre N-terminal split-Cre CMV Mammalian Hirrlinger 106369 pCMV-Tag3B-CCre C-terminal...106370 pCMV-Tag2B-NCreERT2 N-terminal split-Cre-ERT2 CMV Mammalian Hirrlinger 106371 pCMV-Tag3B-ERT2CCre ...homology arms Mammalian Heller 113837 pCAGGS-mTagBFP2-T2A-iCre TagBFP, iCre CAG Mammalian Capecchi 113849 pCAGGS-pac-T2A-iCre...

...analogs Mammalian TAG Huiwang Ai 160041 pRaGE Pyl TAG GFP Y35TAG PylRS M. mazei Bacterial TAG Lital Alfonta...DiZHSeC Mammalian TAG P. Chen 92047 pCOTS-pyl-GFP(35TAG) PylRS M. mazei Cyanobacterial TAG Lital Alfonta ...Alfonta 160089 pPaGE Pyl TAG FliC T248TAG PylRS M. mazei Bacterial TAG Lital Alfonta 160377 pDule-Mb haloTyrRS... Bacterial TAG Peter Schultz 48696 pANAP AnapRS E. coli fluorescent AA, Anap Mammalian TAG Peter Schultz...coli pBof Mammalian TAG Huiwang Ai 68292 SepOTSλ SepRS9 E. coli Sep Bacterial TAG Jesse Rinehart 71403...Bacterial TAG Dieter Söll 104069 pTECH-chAcK3RS(IPYE) AcK3RS Chimeric Nε-acetyl-L-lysine Bacterial TAG David...Bacterial TAG David Liu 104071 pTECH-MmAcK3RS(IPYE) AcK3RS M. mazei Nε-acetyl-L-lysine Bacterial TAG David...

...EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR 26106 pNH-TrxT GU269914 Hexahistidine tag, thioredoxin...EF456736 Hexahistidine tag with TEV cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV...pNIC-CH EF199843 Hexahistidine tag, AmpR 26103 pNIC28-Bsa4 EF198106 Hexahistidine tag with TEV cleavage...GU452710 Hexahistidine tag , Z-basic, TEV cleavage, AmpR 26108 pFB-LIC-Bse EF199842 Hexahistidine tag with... EF442785 Hexahistidine tag with Thrombin cleavage, KanR 26101 pET28GST-LIC EF456739 GST-tag and hexahistidine...hexahistidine tag with Thrombin cleavage, KanR 26096 pET28-MHL EF456735 Hexahistidine tag with TEV cleavage...pCW-LIC EF460848 No tag, double ptac promoter, AmpR 26099 pFBOH-LIC EF456740 Hexahistidine tag with Thrombin...

...Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells...with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure...has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in human cell...You May Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization...collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines...researchers will be able to generate fluorescently tagged proteins of key cellular structures in human cell...TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres (M-line tag) 114413 MYL7-mEGFP AICSDP-46 mEGFP MLC-2a Sarcomeric...

...can be found in Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of...plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins...Materials Fluorescent Protein Plasmids & Resources FP Tagging FP Overexpression Resources This comprehensive ...allows for constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive...Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One. 2013 Jul 2...KS. Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast. 2004 Jun;21...

... mammalian) Tags (e.g. 6xHis, Flag, StrepII, HA, Myc, SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or V5)...pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Resources Read Addgene's Blog...