...add 57 mL of 1.46 M sorbitol, 7.5 mL HI-FBS, and 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium Bicarbonate... 2, Corning 3269, 1272 cm 2 Heat-inactivated FBS (HI-FBS) Pro-Tip Different brands and lots of FBS can... sorbitol (optional) : DMEM low glucose + 1% v/v HI-FBS + 0.1 M sorbitol In our hands, we have found ...sterile 250 mL bottle. Aliquot 774 mL of DMEM + 2% HI-FBS into a sterile 1 L bottle. Add each plasmid DNA...solution to the bottle containing 774 mL of DMEM + 2% HI-FBS. Mix well. Take the CS5 out of the incubator...L-alanyl-L-glutamine (or alternative stable glutamine such as glutaGRO, Corning 25-015-CI) Opti-MEM, Thermo Fisher 31985...L-alanyl-L-glutamine (or stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add ...

...N-terminal 6x His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 Tac (lactose...pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally His-tagged TALEN...-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally His-tagged TALEN fused...artifacts!), photoactivatable tags for super resolution imaging, epitope tags like His for easy western blotting...recombinant protein with an N-terminal 6x His tag and an MBP tag. Contains a signal sequence to localize...different combinations of His and Strep tags as well as N-terminal PelB tags for localization to the periplasm...and C-terminal Strep, His, MBP, TRX, GST, SUMO, MISTIC, and LSL tags as well as tags for protein localization...

...Prey Cd4d3+4-COMP-b-lac His-tag Cd4d3+4-6his Bio-linker-his Cd4d3-bio-linker-6His* FLAG prey CD200-Cd4d3...COMP-blac-3xFLAG-6his Cd4d3+4-pentamer BasiginCd4d3+4-COMP-his-stop Type II expression CD69-His-bio Multi TM...as both monomeric baits and multimerised enzyme-tagged preys. Ectodomain inserts: The inserts that encode...expression vectors which have different C-terminal tags. Most vectors are based on the pTT3 plasmid backbone... TM expression CD36-bio-His Plasmid collections exist for: Basic AVEXIS : A list of all you need to get...Martin S, Söllner C, Charoensawan V, Adryan B, Thisse B, Thisse C, Teichmann S and Wright GJ Molecular & cellular...receptors Human erythrocyte Babesia microti blood stage proteins P. falciparum sporozoite cell surface and...

...purification based on the polyhistidine tag. What is the polyhistidine tag? The polyhistidine tag is a string of ...Benefits of HIS tags In addition to providing a way to purify a protein of interest, polyhisitidine tags can ...low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended if... isolated.   Prior to the polyhistidine tag, alternative fusion tags were used, such as S. aureus’... conditions.   Find polyhistidine tag plasmids here! Where should I place the tag and how many do I need...plasmid containing a polyhistidine tag, you’ll need to decide whether to add the tag to the N- or C-terminus... however, tag placement can affect protein function. B, The string of histidines in the tag is typically...

...mammalian expression 6xHis Epitope Tag pEZYmyc-His - C-terminal Myc-His tag for mammalian expression... His tagged bacterial expression vectors pET His6 TEV - N-terminal His6-TEV tag for ... araBAD p15TV-L - His-tagged bacterial expression vector (see tagged expression vectors...C-terminal 3xHA tag for yeast expression Myc Epitope Tag pKMyc - N-terminal Myc tag for mammalian...series - Expression with Strep, His or optimized YFP tags for purification or localization...promoter pCS2 mt-GFP - Create myc-tag, GFP-tag fusions for transcription pCSDest - Easy cloning...Gateway recombination Back to Top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools...

...optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian...optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian...Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian optimized... suitable for creating individual fluorescently tagged proteins to study protein-protein interactions ...

... (CAPTURE.) Tag : Multiple methods make it easier to tag endogenous loci with epitope tags or fluorescent...include mutations seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed... not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also be used to purify a genomic locus... EM, Myers RM. 2015. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res . ... CRISPR CRISPR History CRISPR History and Development for Genome Engineering ...

...covered! His His tags, often called polyhistidine tags, typically include six consecutive His residues...Diagram of His-tagged protein purification work flow by nickel column.    Affinity tags vs. other ...other tags There are many different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent...blog Plasmids 101: Protein tags Antibodies 101: Epitope tags To Each HIS Own ...planning, affinity tags can make protein purification a cinch. Types of affinity tags, the proteins they...are all reviewed here. What are affinity tags? Affinity tags are used primarily as protein purification... common tags available. Maltose binding protein Maltose binding protein (MBP) is a 45 kDa tag which can...

...Trono 14666 pQE9-His-p97(wt) VCP His T5 ALS Graham Warren 14667 pQE9-His-p97(QQ) VCP His T5 ALS Graham Warren...17229 pQE9-His-p97deltaD2 VCP His T5 ALS Hemmo Meyer 17230 pQE9-His-p97deltaD2(K251A) VCP His T5 ALS Hemmo...0005 VCP His T7 ALS Axel Brunger 12339 pBD-0071 VCP His T7 ALS Axel Brunger 12344 pBD-0010 VCP His T7 ALS...0016 VCP His T7 ALS Axel Brunger 12346 pBD-0013 VCP His T7 ALS Axel Brunger 12347 pBD-0006 VCP His T7 ALS...0009 VCP His T7 ALS Axel Brunger 12349 pBD-0070 VCP His T7 ALS Axel Brunger 12350 pBD-0069 VCP His T7 ALS...0008 VCP His T7 ALS Axel Brunger 12352 pBD-0007 VCP His T7 ALS Axel Brunger 12353 pBD-0018 VCP His T7 ALS...0020 VCP His T7 ALS Axel Brunger 12355 pBD-0022 VCP His T7 ALS Axel Brunger 12356 pBD-0024 VCP His T7 ALS...

... cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of...inexpensive to assay, and easy to purify using histidine-tagged constructs and routine protein and molecular...map, a TEV cleavage site is present between the 6xHis-tag and the coding region of MDH. For detailed clone...

...-His 52067 GSN-bio-His 52068 NID1-bio-His 52069 P4HB-bio-His 52070 PDIA3-bio-His 52071 PDIA4-bio-His 52072...TTR-bio-His 52175 AHSG-bio-His 52176 ALB-bio-His 52177 APOH-bio-His 52178 C12-bio-His 52179 F2-bio-His 52180...ITGA2B-bio-His 53356 OTOA-bio-His 53358 ENTP-bio-His 53359 P2RX1-bio-His 53360 P2RX6-bio-His 53361 ECE1-His-bio...bio-His 53426 F5-bio-His 53427 PLG-bio-His 53428 PLUNC-bio-His 53429 VTN-bio-His 53430 WNT11-bio-His 61486...ACVR1-bio-His 51624 PECAM1-bio-His 51639 SELP-bio-His 51640 ACVR1B-bio-His 51641 ADAM10-bio-His 51642 APLP2...APLP2-bio-His 51643 APP-bio-His 51644 G6B-bio-His 51645 GP5-bio-His 51646 CD226-bio-His 51647 CD34-bio-His...bio-His 51648 CD40-bio-His 51649 CD84-bio-His 51651 DAG1-bio-His 51749 EFNB1-bio-His 51750 EPHB1-bio-His...

...affinity tag is the polyhistidine (His) tag. Small and unlikely to affect function, His-tagged proteins...usually using a Ni2+ column. Like other affinity tags, a His tag can be fused to either the N- or C-terminus...Combo and cleavage tags Frequently, a single tag is not enough. What if you need one tag to increase solubility... to a purification scheme, His tags are frequently combined with other tags for dual-labeling. The problem...to all tags, but rather a quick overview of why scientists use tags, with a few time-tested tags and fusion...length a polyhistidine tract does not greatly alter the size of the tag.   Table 1: Common protein tags ...keep your protein soluble.  Tags for affinity and purification An affinity tag, generally a relatively small...

...RAMA-bio-His 50800 Prohibitin-bio-His 50801 PF10_0166-bio-His 50802 GLURP-bio-His 50803 MSP3.5-bio-His 50804...MSP3.6-bio-His 50805 MSRP5-bio-His 50806 MSRP4-bio-His 50807 MSP8-bio-His 50808 PF13_0125-bio-His 50809 PF14...0044-bio-His 50810 PFA0135w-bio-His 50811 PFA0445w-bio-His 50812 SERA1-bio-His 50814 SERA2-bio-His 50815 ...SERA3-bio-His 50816 SERA4-bio-His 50817 SERA5-bio-His 50818 SERA6-bio-His 50819 SERA7-bio-His 50820 SERA9...-bio-His 50821 PFA0210c-bio-His 50822 PFB0475c-bio-His 50823 PFD1130w-bio-His 50824 RIPR-bio-His... invasion of the human erythrocyte by the blood stage of the malaria parasite, P. falciparum . Please ...

...Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene...empty plasmid backbones with different fluorescent tags for you to create fusion proteins with your gene...Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian Expression...dimerization pCEP4YPet-MAMM - Mammalian Expression pYPet-His - Bacterial Expression YPet-N1 - Mammalian Expression...Monomer pmiRFP670-N1 - Mammalian Expression pBAD/His-miRFP670 - Bacterial Expression iRFP670 643 670 13...Monomer pmiRFP703-N1 - Mammalian Expression pBAD/His-miRFP703 - Bacterial Expression iRFP702 673 702 8...

...Tallahassee Democrat in a story about his life’s work and the stage IV lung cancer that has now prompted...State University has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive... all those collaborations over the years, he and his colleagues built a Molecular Expressions collection...spectra. A comprehensive list of cell markers all tagged with mEmerald. Plasmids to monitor protein-protein...available targets since the Davidson lab always tagged new proteins with mEmerald first, Baird says. From...cell membrane, this collection has fluorescently tagged versions of many proteins known to localize to ...prompted him to deposit the tools he and his team created with the research community at large, "[Fluorescent...

...which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have a FRET acceptor-binding...for protein purification: the NTA binds to the His tag on your protein and the coupled Cy fluorophore ...C-terminal fusion of a tetracysteine tag. In addition, since these tags are so small, one can insert them... between 80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby...interest: you just don’t know it yet! The poly-histidine tag often used for protein purification is a reliable..., as well as by extending the length of the histidine tag from 6 to 10 residues. While these Cy/NTA conjugates...incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni2+: ...

...Protein C, DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please visit our Tags and Other Markers Antibody... Glypican Antibodies Integrin Antibodies Epitope Tag Antibodies Antibody Plasmids Welcome to the Institute...proteins (glypicans and integrins) and common epitope tags, as well as antibody plasmids to create or express...Reactivity Recommended Applications Browse Epitope Tag Antibodies IPI has developed recombinant versions...versions of antibodies that recognize common epitope tags to help purify, detect, or localize particular proteins...s full catalog of antibodies that target epitope tags and other common cellular proteins. ID Recombinant...

...same kinds of opportunities for his students. But he also found that his teaching responsibilities left...limited time for one-on-one mentoring. His solution? Bringing his research into the classroom. I had the...to hear about how he developed his program. Initially, Provost made his labs completely open-ended, allowing...bacteria, purified them to near homogeneity using a 6XHis tag, and compared their specific activities by measuring...purification and characterization of a fusion protein: Histidine tag, malate dehydrogenase, and green fluorescent...San Diego), decided to take a different approach. His own most impactful learning experiences happened ... opportunity to talk with Provost recently about his development of what is now commonly termed a Course-based...

...Campus in Aurora, Colorado, USA. The primary focus of his lab’s research is the mechanisms by which proteins...Gronostajski is a professor at the University of Buffalo. His research focuses on understanding the structural ...research on diseases of glycosylation, arginine mutagenesis, and neural development. Paula Videira: Congenital...as reporters of NFIX function. They will then mutagenize the NFIX gene and test whether variants of unknown...

...optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian...optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian...Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian optimized...interactions in living cells using a microscope. Historically, molecular interactions were detected by indirect...