...Joanne as she answered questions to provide moral support and to monitor user replies, but I can’t thank ...questions What the moderators did not delete or suppress were the large number of scientist posters with... basic questions and to provide a small bit of support (you are not alone!) for those going through the...see graduate and postdoc programs beginning to support diverse career path development--but the science...

...you. One such option is the CRISPR Design tool supplied by the Zhang lab. This simple website invites ...the sgRNA is simple and easy. Several companies supply gRNA sequences, such as GenScript, ThermoFisher...you can order them yourself through whatever DNA supply company you currently use. You can also find many...selected gRNA’s, and has the bonus benefit of supplying you with primers for validating your edit in mice...

...Geneva (Creed et al., 2015) used optogenetics to support these existing findings by controlling neurons ... animal is placed into a tank of water with no support from below or anything to hold on to. In general..., 2007). A study from 2014 (Bahi et al., 2014) supports the relevance of BDNF in depression. The researchers...Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of microRNA124a in the hippocampus evokes antidepressants-like...

...include a note as to where readers may locate data supporting the research reported in PLOS publications. The...available for anonymous request.  This means that supporting datasets must be logically annotated and arranged... in scope. For images, the number of examples supporting the published material is a factor of practicality...over time such that prior formats are no longer supported/readable. Is the author or their host institution...

... of two ways. First, co-expression of a suppressor tRNA (SupD) instead of the SepOTSl can encode a non-phosphorylated...protein as well as context-specific effects of UAG suppression, as described (6). To determine the stoichiometry...pCRT7 NT TOPO E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical ...

...SunTag system, named after the "stellar explosion SUperNova", is a synthetic scaffold that recruits up to ...combinations of states. Each of the integrases is supplied on its own expression plasmid or in various combinations...combinations, while the cognate att sites are supplied as individual reporter constructs or as the fully...voltage indicators: QuasAr1 and QuasAr2 (quality superior to Arch). Both QuasAr1 and QuasAr2 possess mutations...the sgRNA (i.e. TOP1 or TOP2 constructs; see Supplementary Note 3 in the article for more info) are available...homology directed repair templates (see the supplementary info from the paper for details on repair template...excitation light source. Instead, coelenterazine is supplied to the culture media as a chemical substrate for...

... found that in the right environment and with supportive mentors, being open and vulnerable about my struggles...have often provided me with much-needed emotional support during my journey. When you are hunting for labs...join, one of the vital things to look for is a supportive environment—it makes all the difference in a ...scholarship is also a national scholarship that supports students interested in pursuing a career in research...

...genes (e.g. tagging genes representing a protein super family). To overcome these challenges, we set out...RNAs can also be purchased from various other suppliers with a typical turnaround time of 5-7 days. The...reagents could be purchased directly from commercial suppliers without investing time and labour on reagent preparation...gene-specific crRNAs and matched ssODNs from commercial suppliers of CRISPR reagents (turnaround time 5-7 days);...

... that position themselves for super-resolution usages, such as superior brightness, photostability, and...dyes have been specifically designed for use in super-resolution microscopy techniques, such as Stimulated...provide brighter and more stable fluorescence for super-resolution applications. Other current advancements...

...Joanne Jary from Lab Support at the John Innes Centre, and submitted by the Lab Support Team at the Centre...Centre.     Lab support team @JohnInnesCentre rule pic.twitter.com/WMb97fpUOI— Sam Mugford (@samtmugford)...

...plasmid. Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors...vector does not contain Cas9, so you’ll need to supply it with another plasmid.   Figure 3: Comparison...genomic modification. In this system, Cas9 must be supplied on a separate plasmid. Multiplexing in Drosophila...from two Drosophila U6 promoters. Cas9 must be supplied on a separate plasmid. Multiplexing in E. coli...

... NIH-funded researchers and utilizes grants to support our own initiatives, Addgene is deeply concerned...with partners across the research ecosystem to support the essential work of our scientific community ...funding changes are impacting your work and what support would be most valuable from Addgene during this...

...and professional experience are prized skills. Supervise undergrads or less experienced grads Manage a ...something big like plan a conference Recruit many supporters and mentors that will speak for you (and you ...get real feedback Write whenever you can, find supportive editors so your first drafts are always excellent...

...planning meetings with each trainee to create a supportive, growth-oriented lab culture. The aim of these...summarized briefly below (and available of in the supplement of this paper), help each person organize his...Strategies to Resolve Conflict With Difficult Supervisors Management for Scientists: Seeking Feedback Management...

...metastasis CRISPR screen. The Chen Mouse Tumor Suppressor Gene CRISPR Knockout Library is an AAV CRISPR...CRISPR knockout library targeting mouse tumor suppressor genes (TSG). Find the Mouse Metastasis CRISPR-Cpf1...Double Knockout Library Find the Mouse Tumor Suppressor Gene CRISPR Knockout Library If you have a new...

... is resistance to change, public support is weak, government support is “feckless”.  So what can be done...from Harvard Medical School and a long list of supporters from all of the research centers in Boston.  ...

...results section, but you had to dig through the supplemental files to see the actual data. Now you’re wondering...datasets, which are often obscured in large supplementary data files, BioGRID ORCS makes it possible for...sgRNA-level sequence read scores. The original supplemental data tables available with the publication are...

... high school friends. In my teenage years I was super shy - there was even a time when I was too nervous...writing scientific manuscripts and, as one of my supervisors warned me, the swift gratification of publishing...roles can include positions, like mine, that help support academia but the skills researchers gain doing ...

...research area is not as important as choosing a supportive, positive role model. By contrast, as a postdoc...is crucial that you choose an advisor that will support your growth in your science career. Will you be...thinking about a non-academic path, will the PI support this path and help you pursue any career alternative...

...during the pandemic and in the future, depends on support from the scientific community. Deposits of new ... be deposited into repositories, and financial support to maintain repositories’ capacity for storing,...all ways in which the scientific community can support repositories. Repositories are essential for the...