...cells with the CRISPR library in vitro before transplanting them into mice. For in vivo screens, mice are...another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog like SaCas9. You...

...Mapping antibody-escape mutations with the yeast display library. From Starr et al Science., 2021. SARS-CoV... designed so that the SARS-CoV-2 spike RBD is displayed on the yeast cell’s surface. The Bloom lab used...

...vitro (see figure 1B in Terskikh et al.) and even displays predictable kinetics over 14 hours of expression... table: Fast-FT, Medium-FT, and Slow-FT. These display progressively longer blue fluorescence maturation...

...storage. What we don't have are the keyboards and displays. We don't have good ways of converting typically...is the next priority; we need the keyboards and displays for control and visualization. Addgene: What do...

...penetration Less resolution due to larger label displacement Require separate incubation of 1° and 2° Must...multiple dyes Greater resolution due to lower label displacement 2° incubation can be skipped by pre-binding ...

...mismatch at that position. PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from...is more precise than shRNA knockdown, it still displays substantial off-target activity. By mutating residues...

...their characterization of two Cpf1 orthologs that display cleavage activity in mammalian cells. Like Cas9...Cpf1 candidates, Zetsche et al. found two that display robust cleavage activity similar to that of Cas9...

...tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split EGFP has also been used to study...

...light-sensitive systems. Most excitingly, assembly of the split luciferase is reversible, making PILATeS suitable...., Wang, B., Prakash, M., & Dunn, A. R. (2024). Split Luciferase Molecular Tension Sensors for Bioluminescent...

... cells correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients... recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23 Jhunjhunwala...

...is a significant need for healthy livers for transplants. Growth of new livers in culture could resolve..., neither of these can substitute for a liver transplant. My lab will combine bioengineered scaffolds ...

...environmental triggers. This is done by creating a split dCas9 protein that can reassemble when exposed to...015-0784-0 Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and...

...authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression, and sensitivity...from Twitch-2B (Matlashov et al. 2022). iGECInano displays better response kinetics and photochemical characteristics...

...protein-protein interactions, we recommend using NIR split reporters based on miRFPs (Shcherbakova et al., ...and higher contrast than previously developed NIR split probes (Tchekanda et al., 2014, Filonov et al., ...

...popular tools for studying frogs include egg transplants and microinjections. Injections of mRNAs, for...disease-relevant genes in such a controlled environment displays how essential the frog is as a biomedical model...

...to a number of rare genetic diseases caused by splicing defects, including ataxia telangiectasia, congenital...Exon-skipping antisense oligonucleotides to correct missplicing in neurogenetic diseases. Nucleic Acid Ther. ...

... effective in both NHPs and mice, but they can display species-specific tropism. For example, AAV9-X1.1...strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive..., lung, and thymus, and transduce the heart and spleen at similar efficacy levels to AAV9 (Stanton et ...

...viewers. Use a colorblind-friendly color palette for display (like green-magenta-blue or orange-blue rather ...>Replace Red with Magenta. Or you can use Color>Split Channels and then Color>Merge Channels into whatever...

...tend to be choc-full of open reading frames that display little to no homology to anything else, making ...amplification (by a mechanism known as multiple displacement amplification) even in the presence of certain...

...during the differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic.... Berns, and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing ...