... is usually accomplished by Cas9-mediated dsDNA breaks: following a cut, the error-prone nature of non-homologous...although this is rarely done). When targeting a dsDNA break for HDR, the choice of target site is far more constrained...which makes DNA changes in the absence of dsDNA breaks (Rees et al., 2018). For C>T and A>G base editors...

...marker like the Hoechst stain for the nuclei. (See Brewer et al, 2022 for a sample protocol.)  Simultaneous... Nguyen, K., Van Der List, D., Murray, K. D., Engebrecht, J., & Trimmer, J. S. (2019). A toolbox of IgG... 8, e43322. https://doi.org/10.7554/eLife.43322 Brewer, M., McDonough, L., Zhu, Y., Neumann, E., De Caestecker...

...homology arms of 35-80 bp, and DNA double-strand breaks up to 54 bp from the desired insertion site result...knock-in efficiency, presumably by channeling DNA breaks into the homologous recombination repair pathway...interesting to note that recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle...

...protein, first block the Golgi apparatus with Brefeldin A to inhibit secretion and then follow the typical...NK) cells.   Tips for flow cytometry success Break up cell clumps Success in a flow cytometry experiment...the sample through a nylon mesh cell strainer to break up clumps before running on the instrument. To sustain...

...development (Grimm & Lavis, 2022). With the wide breadth of fluorescent tools that have been developed throughout...is an important consideration for assay design (Breen et al., 2016). When selecting dyes, it's important...(1). https://doi.org/10.1038/s41598-020-78827-9 Breen, C. J., Raverdeau, M., & Voorheis, H. P. (2016)....

...al. 2022. Image created with BioRender.com. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory.../ENEURO.0373-21.2022 Brown, D., Altermatt, M., Dobreva, T., Chen, S., Wang, A., Thomson, M., & Gradinaru...Kumar, S. R., Miles, T. F., Chen, X., Brown, D., Dobreva, T., Huang, Q., Ding, X., Luo, Y., Einarsson, P... Kumar, S., Miles, T. F., Chen, X., Brown, D., Dobreva, T., Huang, Q., Ding, X., Luo, Y., Einarsson, P...

...other organisms, CRISPR-induced double stranded breaks are lethal because the non-homologous end-joining...ineffective in bacteria, CRISPR-induced double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini...genomic locations, and introduces double strand breaks, the bacteria are unable to resolve them, and the...

...HDR), the pathway for resolving DNA double-strand breaks (DSBs) preferred by researchers. Compared to non-homologous...Base editors chemically modify DNA bases without breaking the DNA backbone, sidestepping the problem of ...converts a C to U without producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com...

...talk to scientists from around the world making breakthrough discoveries!” I knew right then I had found .... Summaries of the latest technologies and breakthrough research. Insights about how to navigate through...

...Horticulutre Biotechnology, he did his PhD in Plant Breeding, Genetics, and Biotechnology at Michigan State...collaboration across the globe. Listen here! Podcast Breakdown (Time: Topic) 0:00-1:00 : Intro 1:01-3:48 : Kingdom...

...proteins, read our recent blog post. Check out the break down below the audio player to jump to specific ...Happy Listening! Click here to listen! Podcast breakdown: (Time : Topic) 0:00-1:00 : Introduction 1:01-...

...We’re breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward ...plasmids through this podcast segment. Below, we break down each of the Hot Plasmids segments into its ...

...interview with Dr. Koeris below. Check out the breakdown below the audio player to jump to specific subjects...listening! Download the podcast here! Podcast breakdown (Time : Topic) 0:00 - 1:25 : Introduction 1:25...

... five strains of Ebola representing the latest outbreak and earlier ones, along with links to the relevant...content. -And the latest updates on the ongoing outbreak from the CDC and WHO. - For anyone interested ...

...most CRISPR systems and generates double strange breaks at target sites 5' of the 5'NGG3' PAM sequence ...which nicks DNA rather than creating double stand breaks and may better facilitate homology directed repair...

...empowering researchers worldwide. Countless breakthroughs enabled by shared resources. As we step into...shared, every collaboration formed, and every breakthrough enabled brings us closer to making an impact...

...gather the results of Myriad's genetic tests for breast and ovarian cancer risk directly from customers...favorite ways to share science. Image credit: Judy Breck, via flickr  ...

...accomplishing it all. How do we encourage people to take a break from their everyday tasks and make time for special...However, I think it’s just as important to take a break from work to share time with the people around us...

...yeast research, a few of those have been trips to breweries who happily exchange our expertise with lots of...the lab, it is important to take the occasional breather and acknowledge the team’s personal and professional...

...this system, Cas9 generates a double-stranded DNA break at the genomic loci of interest and on either side...complementary to either side of the double-stranded break in the genomic loci of interest. As the universal...