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Showing: 741 - 760 of 774 results

  1. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage...

  3. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...were having a positive experience, I was still incredibly nervous about leaving academia. A lot of academics...

  4. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...

  5. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation...

  6. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...17,560 CRISPR plasmids. That’s almost a ninety-fold increase in size! From this collection, we’ve shared almost...

  7. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for the membrane, where it will ...

  8. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...

  9. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...starting vector that the depositing lab modified to create their new plasmid.  The Addgene Vector Database...

  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...addition, there is a drop in image brightness with increasing depth caused by light scattering and absorption...

  11. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ... the time it takes the FP to correctly fold and create the chromophore. This can be from a few minutes...

  14. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and E3 (Early...

  15. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...don’t have to optically magnify quite so much. Created with biorender.com Steps 2-4 are not found in...

  16. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...AAV-RPE65 treatment, famously visited Congress to help increase awareness about the potential of gene therapy!...
Showing: 741 - 760 of 774 results