...experiment will likely depend on: The protein you are trying to purify The expression system If you want the...fusion proteins from being purified by GST (maybe try MBP if you are having solubility issues!). Need a...

...protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating...downstream of the open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more...

...correct plasmid, scientists would spend hours to days trying find their correct clones. Additionally, the presence...cassette within the coding region of the gene they are trying to disrupt or delete, which both inactivates the...

...questions. Talk to as many lab members as possible. Try to get them in a setting out of the lab (coffee, ...Unfortunately, a bad mentor can be bad news for anyone. Try to speak to them more than once to get a full picture...link from 2020) on this topic), for this second entry in the “Mentoring for Scientists Series”, let’s ...

...variation (CNV) analysis of the U2OS cells. Why try CRISPR-Sirius? With all the tools out there to visualize...visualize genomic loci brightly, give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References...

...fluorescence (for some background on fluorescence, try the Introduction to Fluorescence Microscopy video...that work in one context may not work in another. Try to choose primary antibodies that have been validated...time to carefully review your options. If possible, try to select a couple of antibodies that seem promising...see associated with IF are immunocytochemistry (ICC) or immunohistochemistry (IHC). All three of these ...

...example, if your work requires specialized equipment, try finding institutes that offer shared equipment that..., instead of solely discussing numerical values, try to keep conversations focused on research relevant...needs and limitations. Everything is negotiable. Try asking for discounts, collaborations, access to internal...sites (like, indeed.com, or salary.com). Search industry and academic job titles in the regions where the...

... enough - but this week we’re certainly going to try!      Here’s our Depositor Week schedule:  Monday...

...vector is large (>10 kb) or has a low copy number, try following protocols for low copy plasmids. For example...plasmid and the recombined vector backbone, you can try re-streaking for single colonies and testing multiple...

...well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the Flowtime Technique....these tips are helpful to you no matter what you’re trying to accomplish during this period. ...

...researcher decides on a new project and begins by trying to reproduce someone else’s result. Then, they ...that they thought would be moving forward instead trying just to get going. It’s like being stuck in jail...

...Relationships for Success”. Choose 1-2 tactics to try. One of my favorites is making the pledge to have... being an educated mentee. Other opportunities: Try virtual mentoring at Mentornet.net. This is an online...seeing a lot of success at universities around the country with groups who are piloting this. Watch for my...

...occasional errors. That can be advantageous if you are trying to knock out a gene. One can also exploit homology-directed...induced pluripotent stem (iPS) cells from patients and try to compare those lines, they may all have the mutations...

....  We hope you will give our new search engine a try to find reagents and educational material. Let us...us know what works for you and what doesn’t. Try out the new Addgene search engine! Additional resources...

...molecular cloning. Depending on whether you are trying to overexpress or knockdown a gene, you might need...not always clear which side he roots for. You can try to convince him to use Twitter @jonchowphd but he...

...RNA structure and the site in the genome you are trying to target play a large role in Cas9 activity and... CRISPR mutant of interest, give these methods a try.  References Højland Knudsen C, Ásgrímsdóttir...homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074/jbc.m117.777722...

...how the scavenger hunt works works: You will be trying to find pictures of Addgene pets with Addgene-branded...

...summarize, but we wanted to mark the occasion by trying.  When we started in 2004, we were the plasmid...

...gloves can sometimes be annoying. To make it easier, try this quick trick. Just flip, and press! Lab tip...

...that blue and violet light can penetrate. How can I try using photoswitchable kinases in my experiments? ...into loops of a protein of interest. If you plan to try this, it’s important to check that the function of...