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Showing: 41 - 60 of 107 results

  1. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...authors estimate that a gravity-based column plasmid maxiprep kit takes 1-1.5 hours to complete, while this ...plasmid prep kits come in discreet sizes (mini, midi, maxi, giga) which requires you to buy different kits ...

  2. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...s illumination (see Figure 2). H describes the maximum signal strength, while W results from the time ... The resulting histograms is described through maximum current (H), the time the cell needed to pass through...

  4. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...bloggers Dominik Paquet and Dylan Kwart from Ludwig-Maximilians-University in Munich and Marc Tessier-Lavigne...Institute for Stroke and Dementia Research, Ludwig-Maximilians-University Munich. His lab develops iPSC-based...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... guide RNA components (crRNA and tracrRNA) for maximum Easi-CRISPR efficiency. Easi-CRISPR is compatible... as possible to your desired insertion site to maximize HDR efficiency. In this case, we’re fortunate ...

  6. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...acids close to the C’ terminus of the protein, to maximize the chances of creating a non-functional allele...Selection of gRNAs for an experiment needs to balance maximizing on-target activity while minimizing off-target...

  7. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...population level, but each individual cell only has a maximum of one gene knocked out. In this genetically heterogeneous...Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

  8. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...UNG) from E. coli. The resulting construct, eUNG-BE4max(R33A)ΔUGI, was given the pithier name CGBE1, for....2% of studied sequences) (Huang et al., 2024). BE4max, a CBE, generated similar deletions at a lower ...

  12. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...academia How can we shape our time in academia to maximize productivity and success? We asked leading professors...

  13. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...potassium-selective channelrhodopsins. HcKCR1 has a maximum absorption of 540 nm and two-photon excitation ...

  14. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...10 gRNAs per gene. The guides are optimized to maximize on-target cleavage and minimize off-target effects...

  15. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...intelligent discussion, and/or brainstorming sessions to maximize productivity and direction of the project? What...

  16. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...need to add an additional 'G'. Q3: What is the maximum amount of DNA that can be inserted into the genome...

  20. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ... of sgRNA. Generation of sgRNA libraries, with maximum complexity, is achieved with synthetic oligos with...
Showing: 41 - 60 of 107 results