...article in the BBC Wildlife Magazine. It is easy to see how these incredible images are attracting so much ...interest for its stunning images that is has been featured in Time magazine . Other smaller competitions...an aesthetic level as well. Beautiful microscope images which traditionally languished in the pages of ...within flowers with the use of x-rays. His haunting images have been enchanting people for years and have ...featured in the National Geographic and Smithsonian Magazine, where the editor stated “Albert G. Richards, ...

... challenges in 2-photon in-vivo imaging of voltage fluctuations. Image credit: Liu Z, et al. Cell. 2022...found an indicator that is optimized for 2-photon imaging of voltages. This includes monitoring of fast bursts...in mouse. C) Action potential frequencies with (magenta) and without (black) photoactivation of HcKCR1 ...top) and with (bottom) photoactivation of HcKCR1. Images adapted from Govorunova et al. 2022.     HcKCRs...know. We’d love to hear from you!   Fig. 3: Image of Addgene’s western blot for the myc-tagged protein...on the Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody ...

... our blog. Image: The final and required step requires submission of an experimental image result that... with a caption. For microscopy images, please include channel imaged, a color legend for probes/dyes/...promoter, or tool type and each entry contains an image along with key experimental details (e.g. titer,...and objective used. If you did not generate the image yourself and it is under copyright, you will need...owner and will have the opportunity to cite the image to give proper attribution.  We hope that this ...

...from traditional imaging to FRET and stochastic single-molecule super resolution imaging. mNeonGreen also...yellow fluorescent protein wavelengths, it can be imaged either with standard green fluorescent protein ...2.6-fold increase in signal relative to mCerulean imaged with a standard CFP set. mTFP1 can be used as a...donor to mCitrine and mOrange or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive...characterization and applications in fluorescence imaging." Biochemical Journal 400.3 (2006): 531-540. PubMed...determinants of color and applications in fluorescence imaging." BMC biology 6.1 (2008): 13. PubMed PMID: 18325109...

...demonstrated through living images created by the team (Fig. 2). These images are based on the concept of...bacteriograph was created using 160 individual images stitched together. Image from Romano et al., 2021 with permission...bacteriographs created about a decade ago. To create these images, the scientists used a strain transformed with ...mask over the plate containing the pattern of the image, and shined blue light over the plate. Areas on ...areas that are exposed will fluoresce. With these images, you can see just how spatially precise the activation...

...and green channels overlaid on a bright-field image. Image from Ma et al, 2016. Using dCas9 to visualize...represents a gRNA targeting a specific chromosome. This image represents the composite of fluorescent measurements...Red boxB-boxB Cyan MS2-PP7 Yellow PP7-boxB Magenta boxB-MS2 White boxB-MS2-PP7 Despite the success...will produce a secondary color cyan, yellow, or magenta, depending on the hairpin combination bringing ...different hairpin sequences produce yellow, cyan, or magenta light. A gRNA with all 3 distinct hairpin sequences...  References 1. Chen, Baohui, et al. “Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized...

...integration, producing a second copy of the sgRNAs. Image reused from Walton et al. 2024 under a CC-BY-NC-...editing efficiencies at different loci in U2OS cells. Image adapted from Yan et al. 2024 under a CC-BY license...against vinculin as a loading control (right panel). Image from Addgene. These antibodies are recombinant...conjugated to a JaneliaFluor dye emitting at 525 nm. Image reused from https://www.janelia.org/open-science...Cre-dependent AAV1 vectors and are suitable for extended imaging of neurons in vivo. Because they are negative-going...    Figure 5: A) Representative confocal images showing GFP expression from ssAAV5s with hIBA1 ...splice acceptor site; pA, polyadenylation signal. Image reused from Riedmayr et al. 2023 under a CC-BY license...

...2015) or the UnaG protein that binds bilirubin (Kumagai, 2013). Next to these naturally occurring co-factor..., et al. "LOV-based reporters for fluorescence imaging." Current opinion in chemical biology 27 (2015)...2015): 39-45. PubMed PMID: 26087123. 4. Kumagai, Akiko, et al. "A bilirubin-inducible fluorescent protein...11. Várnai, Péter, and Tamas Balla. "Live cell imaging of phosphoinositides with expressed inositide binding...PMID: 18155467. 13. Chen, Baohui, et al. "Dynamic imaging of genomic loci in living human cells by an optimized...Nano-lanterns for real-time multicolor luminescence imaging." Proceedings of the National Academy of Sciences..., Satoshi, et al. "Single-cell bioluminescence imaging of deep tissue in freely moving animals." Science...

...specific subcellular regions, laser ablation can damage other cellular structures, and genetic mutations...body. B) Schematic of Opto-katanin’s use in cells. Images adapted from Meiring et al., 2022, under CC-BY ... Opto-RhoGEF design and activity. B) OptoTIAM (magenta) expressed in a monolayer of endothelial cells,...after blue light illumination. Scale bar 25 µm. Images adapted from Mahlandt et al., 2023, under CC-BY...protein vesicle trafficking via CRY2-CIB1 clustering. Image adapted from Aljiboury et al., 2023, under CC-BY...expressing plasma membrane-targeted SuperPLDx30 (magenta) and PA probe GFP-PASS (green). After 30 minutes...colocalizing with SuperPLDx30. Scale bar 10 µm. Images adapted from Tei et al. 2022, bioRxiv, under a ...

...and consisting of the fusion proteins N713-pMag and nMagHigh1-C714, this new system had both low background... system by modifying paCas9-1 using nMagC714 instead of nMagHigh1-C714, generating paCas9-2. This change...figure below. Their most successful design utilized Magnet photoswitchable proteins derived from the fungal...Additionally, high intensity exposure can cause DNA damage. Far-red light, however, can penetrate 5 mm past... be used to identify specific genomic loci for imaging techniques. Since expression of dCas9 can potentially...gene transcription Nihongaki et al., 2015 paCas9 Magnet proteins from N. crassa Split Cas9, Split Cas9 ...

...bacteria CRMAGE system combines CRISPR and recombineering-based MAGE The Nielsen lab’s CRMAGE system is...Pedersen LE, Sommer MOA, Nielsen AT (2016) CRMAGE: CRISPR Optimized MAGE Recombineering. Sci Rep 6: . https:/...method that combines CRISPR and recombineering-based MAGE (Multiplex Automated Genome Engineering) technology...self-destruction" after induction with L-rhamnose and aTet. CRMAGE is much more efficient than traditional recombineering...simultaneously is possible with efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours...

...depends on both the biological system as well as the imaging method, we recommend basing your selection on a...application. The three key properties for live cell imaging that will be treated in this series of posts are...experimental conditions that are related to the imaging strategy and the sample. Practical brightness A...mammalian cells is to tag an endogenous gene. By imaging cells (or tissues) that produce an endogenous protein... be used to verify performance under different imaging conditions (e.g. different emission filters) or...Choosing Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional...

...this post: Anti-FLAG Frankenbodies for modular imaging of cellular targets Optimized genetic screening...and editing Anti-FLAG Frankenbodies for modular imaging of cellular targets by: Mike Lacy Adding to their...a variety of applications, including live-cell imaging, single-molecule tracking, immunolabeling of fixed... be streamlined into a single array for Cas12a. Image from DeWeirdt, et al. 2021 To validate this ...expressed and coexpressed proteins run as ladders. Image from Santilli, et al. 2021 Plasmids are used...of CasMINI for genome engineering applications. Image from Xiaoshu, et. al. 2021.   Created by the...

...of the antibody binding “distinct imaged pattern.” The distinct imaged pattern should align with what the...contains secondary antibodies, allowing us to only image autofluorescence i.e., background noise. We then...and not from the background noise. If the sample images similarly to the negative control, that informs...on the antibody signal’s location. If a distinct imaged pattern does NOT align with the atlases/literature... own analysis of the background noise, distinct imaged pattern, and exposure times. To test the antibody...

...antibody specificity via a colorimetric reaction. Image from Boguszewska et al., 2019.   Competitive...antibody specificity via a colorimetric reaction. Image from Boguszewska et al., 2019.       Direct ...antibody specificity via a colorimetric reaction. Image from Boguszewska et al., 2019.   Indirect ELISA...antibody specificity via a colorimetric reaction. Image from Boguszewska et al., 2019.   Detecting the...this line to calculate the protein concentration. Image from Jagarlamudi et al., 2015.   The maximum...Urbaniak, S. et al. Review: immunoassays in DNA damage and instability detection. Cell. Mol. Life Sci....

...AC, BC, and ABC). Nielsen Lab E. coli CRMAGE Plasmids: The CRMAGE system is a fast, multiplexable method...12. Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE Recombineering." Sci Rep. 6:19452. PubMed...method that combines CRISPR and recombineering-based MAGE (Multiplex Automated Genome Engineering) technology...self-destruction" after induction with L-rhamnose and aTet. CRMAGE is much more efficient than traditional recombineering...simultaneously is possible with efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours...

...sea anemone's red color can be used for mimicry. Image: Bernard Spragg. Advantages of using chromoproteins...Chromoprotein expression from bacteria on agar plate. Image: Liljeruhm et al., 2018. For each chromoprotein... plasmids expressing amilCP and tsPurple genes. Image: Lazarus et al., 2019. One such lab is the Waldor...chromoprotein is expressed and gives the cell a magenta (amilCP) or purple (tsPurple) color. Though the... used as a FRET acceptor to mRuby2 or mScarlet. Image: Murakoshi et al., 2019. ShadowR is an orange...developed as an acceptor for fluorescence lifetime imaging microscopy-based Förster resonance energy transfer...

...Choosing the best PE6 variant for your application. Image reused form Doman and Pandey et al., 2023 under ...fusion and targeting components are interchangeable. Image from Strebinger et al., 2023, reused under CC-BY... trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv ...GFP-iPAK4 within an hour. Analyzing the ticker tape images could recover transcriptional histories, accurate...labeled with HaloTag-iPAK4. Scale bar 10 µm. C) Images of iPAK4 fibers in cultured rat hippocampal neurons...induces cFos::GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC...

...immunofluorescence and indirect immunofluorescence work. Image created with BioRender.com   Indirect IF is ...access their target and that you will be able to image them in the end. Again, the methods you use will...time for this step as well. Preservation and Imaging Similar to Sample Preparation, this step can ...procedure with key considerations about each step. Image created with BioRender.com   The first couple.... the spectral properties match your available imaging instruments and the spectral properties do not ... pressing research questions, but also stunning images (am I the only one with IF results as artwork at...

...studies that involve surgery or imaging.  Rattus norvegicus. Image from Staffan Vilcans. Rat physiology...Idalia & Bernardo, 2017).  Escherichia coli. Image from NIAID. E. coli is often a part of the “molecular...mexicanum) Ambystoma mexicanum, aka the axolotl. Image from AJC1. The Mexican axolotl is one of the ...biomedical research.  Xenopus, aka African frog. Image from Holger Krisp. The frog genome has high synteny...adaptations.  Arabidopsis thaliana flowers. Image from BlueRidgeKitties. If you are working with...