...etc., microbially or in cell-free systems. These pathways typically involve multiple steps in which a precursor...isolated afterwards. To engineer these production pathways, MoClo has been used to assemble multigene constructs...testing any number in the Level 2 assembly. These pathways can require a lot of optimization - which is why...

...cell viability and increase protein production (Schwarz et. al., 2020). Harvest and purification At its....2013.00217. PMID: 23908655; PMCID: PMC3725456. Schwarz H, Zhang Y, Zhan C, Malm M, Field R, Turner R, ...Epub 2019 Dec 28. PMID: 31891733. Brechmann NA, Schwarz H, Eriksson PO, Eriksson K, Shokri A, Chotteau ...

...vectors - which rely on insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific...Recombination or Alternative End Joining, two alternative pathways. See (Guo et al, 2023) for a review of other targeted...to their location (e.g. in a gene in a similar pathway, a growth factor, etc.) this control is usually...

...several proteins in the DNA oxidation and repair pathways work downstream of Tet1 to restore the DNA after... S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan R, Blancafort P (2015) Stable oncogenic silencing...coupling of methylcytosine oxidation and DNA repair pathways. Nature Communications 10:. https://doi.org/10.1038...

...breaks into the homologous recombination repair pathway. Reagents required to perform pha-1 co-conversion...researchers to quickly and easily start genome editing (Schwartz et al., 2018). To see more CRISPR tools for C.... . https://doi.org/10.1534/genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing...

...demonstrating that this system can also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin... M., Nguyen, T. H., Prathima, P., Fithian, W., Schwartz, M. A., Hacohen, N., Doench, J. G., LaFleur, M...

... will be able to better choose which genes and pathways to target in future small molecule screens. He...coworkers to test inhibitors of a variety of signaling pathways for their effects on the disease. This work will...

...trajectories and identify both up- and down-regulated pathways. Individual cellular fates were then matched with...valuable reference if you are looking to understand a pathway, or just looking for the best astrocyte differentiation...

... in organoid cultures are stable,” said Gerald Schwank, first author of the cystic fibrosis report. “This...Cell. 13, 659-662 (5 December 2013). 8. Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

...endogenous RNAi pathways. These mutations however can interfere with other genetic pathways making it difficult...

...be applied to an important neuronal signalling pathway - if you’ve got a useful tool, there’s likely someway...networks, and selectively activating signaling pathways. Many scientists came to the Addgene booth to ...

...To control for protein degradation due to other pathways, it is standard practice to compare degradation...ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active form of...

...earning their PhDs (3), showing that the ‘standard’ pathway into academia may in fact be just another ‘alternative...recently launched a survey “Identifying Career Pathways for PhDs in Science”, through which she collects...

... in different steps in the glycan biosynthetic pathway and have a wide range of clinical manifestations...lines to study how immune processes and biological pathways are altered in PMM2-CDG. These studies are expected...

...further, his lab is now studying how Argonaute pathways may be involved in preventing silencing of self-genes...self-genes. Dr. Mello presented a hypothesis that these pathways are involved in RNA based surveillance of repetitive...

...isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate functional...Cas9." Cell Stem Cell. 13, 659-662 (2013). Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

... using the endogenous homology directed repair pathway, a new user-defined DNA sequence (like GFP) can...previously unknown protein functions. References Schwartz, Matthew L. and Eric M. Jorgensen. “SapTrap, a...

...associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein might...undergraduate research experiences (CUREs) as a pathway to diversify science. Evolution and Development...

...since essential and non-essential genes for both pathways had previously been identified (Hart et al, Bassik...systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility." Cell 152.4 (...

...genome editing. The homology-directed repair (HDR) pathway is downregulated in mature tissues - no problem... through the non-homologous end joining (NHEJ) pathway. It’s estimated that very little dystrophin correction...