Plasmids 101: E. coli Strains for Protein Expression
Type
Blog Post
Published
Feb. 10, 2015, 3:06 p.m.
...conditions. Many recombinant proteins express better at 30°C or room-temperature, which is accomplished by growing...growing your culture to the desired density at 37°C and reducing the temperature or moving it to a bench-top...toxic or insoluble proteins
Origami2 (DE3)**
Streptomycin and Tetracycline
Contains highly active thioredoxin...
General protein expression
m15 pREP4*, **
Kanamycin (pREP4)
Cis-repression of the E. coli T5 promoter...