... be your actual mentor? Is there a chance the supervisor will be moving on soon?   Will you get to meet...interviewing: Lab members speak disrespectfully of supervisors and/or each other. You are not allowed to speak... before answering questions about lab culture. Support for diverse career paths I hope by now you are ...making machine. Choose one that is a true mentor, supporting career development and growth. Have lab alumni...someone(s) train you technically? Will the PI/supervisor look at your data and suggest follow up experiments...

...corresponds to the supercoiled plasmid monomer, and at <6 kb, consistent with the size of a supercoiled plasmid ...sample. In these instances, please note that the supercoiled dimer band can sometimes overlap with the band...development to examine plasmid topologies (e.g. supercoiled, open circular, linearized, etc.) (Schleef et...Rudolph C. Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian cells. J...

... and logistic support to increase the chances of success, but also nurture a supportive and protective...mentee, where (s)he feels truly understood and supported, and can more fully identify with and be inspired... Vela, who reiterated the Spanish government’s support for the IMP program as well as for the Spanish ...public and private institutions also showed their support and hope for the IMP program. The conference also...

...particles into the culture supernatant and you can generally harvest the supernatant containing viruses 24h...24h after transfection. This supernatant can be stored at 4 °C or can be directly added to your target ... process. You have to centrifuge the culture supernatant in order to precipitate the viral particles. ...Some protocols suggest adding sucrose to the supernatant to create a gradient of density that favours ...

...performance and use super-resolution or single-molecule techniques.   Figure 2: Two-color super-resolution...SNAP-tag and HaloTag ligands are available from suppliers like New England Biolabs and Promega (though if.... If you want to use far-red rhodamine dyes for super-resolution imaging, HaloTag gives better performance...Toomre, D. (2019). Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags...

...programs are still doing an inadequate job of supporting science trainee transition into diverse, successful...amplified by systemic biases which, in the absence of support and structure, prevent science from attracting ... of Massachusetts Medical School. It has been supported by a Steering Committee and working groups with...Institute of General Medical Sciences (NIGMS) will support the development and implementation of collections...

...available in both trial- and full-size, with assay-supported recommended uses and education content. This work...our commitment to top notch quality service and support.  Alongside our material offerings, in 2022 we...expanding our suite of materials and services to support more research and innovation around the world. ...will remain the same is Addgene’s commitment to supporting the research community through sharing science...

...over $100 million in annual R&D expenditures to support increased public access to the results of federally-funded...on the global science enterprise lending their support to open scientific research data around the world...industry concerns.” 5)      In the aftermath of the Supreme Court’s decision to ban gene patents in a case ...online survey of scientists showing overwhelming support for open access to peer-reviewed journal publications...

...Behind-the-scenes support from their bosses and employers. Availability of superiors for consultation,...self-directed, but need precise leadership and support from their superiors (beware of not micromanaging the scientist...

...Unfortunately, the vast majority of antibodies are supplied with no sequence information, leading to problems... specific activity for each reagent batch. To supplement this, Addgene will be creating a crowdsourced... new services. “We appreciate NIH’s vision and support for this project. This grant will allow Addgene... part of this initiative! The project will be supported by a NIH BRAIN Initiative grant (NS119916). The...

...from the American Society of Plant Biologists and support from the National Science Foundation, ABRC’s outreach...provide in-depth, ready-to-teach lab protocols and supporting materials such as instructional videos and datasheets...classroom is that staff from the ABRC are here to support you! From questions about growing conditions, to...e-mail and phone to answer questions and provide support. Growing capacity - ABRC outreach today Over the...

...Rather than being released into the cell culture supernatant, the viral particles associate with the cell ...be examined. Increased yields with sucrose supplementation is serotype specific In our study we observed...preparations for, we wanted to be certain that this supplementation strategy would have no effect on the purity... we will be spending less time and resources resupplying our current inventory and more time providing...

...strategies for finding what they need, and we wanted to support all of them — searching, browsing, drilling down...experts, our software team tailored Elasticsearch to support the different usage patterns the larger team identified... selecting a suggestion — how should these be supported and combined?  Luckily, we had the advantage of... information in our search indices in order to support different kinds of matches: exact words, partial...

.... But how do you find these elusive teachers, supporters and advisors? I tried to start a more formal ...for their development plans. They all found the support of the group to be intrinsic to their success. ... continue to meet regularly, providing ongoing support, advice and accountability for development goals...mentoring relationship.  Celebrate your successes and support each other as you transition to the next stage ...

... Training (BEST) awards, which are designed to support new approaches that address the need to train our...and from what fields – are needed to adequately supply these demands? Are there other solutions, such ...University of Maryland College Park, is a strong supporter of increasing the number of postdoctoral scientists... collection and analysis. He is also a strong supporter of science Master’s programs. This will be the...

...figure must be clear, concise, meaningful, and supportive of the mission of the application and the funding...gives the writer a chance to delegate funds to supplies, personnel, equipment, travel, etc. As a first... value of costs. It is important to be aware of supply costs per year, how many graduate students and ..., etc. It may be helpful to ask your advisor to supply a budget form used in a previously funded grant...

...population. While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these...will not necessarily express EnvA unless it is supplied in trans. Monosynaptic RABV spread can be achieved...transsynaptic spread can be controlled by selectively supplying the G protein in trans only to starter cells (...replication will then occur and the G protein (supplied in trans) will be incorporated into the viral ...

...which can reduce the potential image resolution of super-resolution fluorescence microscopy. Their size also...about 2 nm, which can lead to higher resolution for super-resolution microscopy. Nanobodies can be labeled... (2015). Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native...DSHB). 1 ml of antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially...

...priorities, but many find it helpful to obtain support and ideas from those who seem to be managing. Being... When the balance tips, we need to look to for support and ideas to manage our stress. If you are saying...club, meet the neighbors and create a network of support to help with elder care, carpooling, cooking on...’ school, and with professional organizations supporting women in science and career development. I try...

...Very often, this is what it means to be a ‘superbug’. Superbugs are often very difficult and very time consuming...it a full month without a new, drug-resistant ‘superbug’ making headlines in one part of the world or ...an important step, as you can’t walk into any supermarket now without seeing a promotion for antibiotic-free...