...plasmid multimerization in the naturally occurring E. coli plasmid ColE1 (Goebel & Helinski, 1968; Bazaral... (Bedbrook & Ausubel, 1976; Fishel et al., 1981). E. coli strains such as DH5α, HB101, NEB Stable, Stbl3...multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7...1566524020666200427110452. PMID: 32338220. Crozat E, Fournes F, Cornet F, Hallet B, Rousseau P. Resolution...during our quality control process. Since MiSeq involves sequence assembly from short reads (<300 bp) which...

...Lentiviral preps for expressing the E protein (pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...interneurons (83895). See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype...Fishell   Chemogenetics Chemogenetics offers noninvasive methods for manipulating cell activity, particularly...

...Burke Lab (Roux et al., 2012). BioID utilizes the E. coli biotin ligase, BirA*, with a site mutation (...DNA-binding domain (233 amino acids) found in the E. coli version. It also contains a single mutation ...signal to noise ratio 30-fold compared to the standard E. coli BirA* mutant. RapID consists of this mutant ...Interactions through Spatial Proximity." Journal of Investigative Dermatology 137.12 (2017): e197-e203. PubMed...

...Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation ...PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers highly...few inventory items offered domestically, but by the end of 2016, we expanded our viral inventory to 25... lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide...pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing... Carolina, Chapel Hill. These can be used to investigate the effects of activating particular signaling...neurons drives feeding behavior in mice. J Clin Invest. 2011 Apr;121(4):1424-8. PubMed PMID: 21364278....

...including the Cre-lox system from the P1 phage of E. coli. Fun fact: There is evidence that lightning ...example, there are plasmids that allow Salmonella and E. coli to infect the human intestine, and other plasmids... plasmid genes can give bacteria the ability to invade environments found on and in living organisms. ...

... a supplemental add-on kit and is compatible with E. coli and/or cell-free systems. Protein expression... et al. “EcoFlex: A Multifunctional MoClo Kit for E. Coli Synthetic Biology.” ACS Synthetic Biology, vol...CIDAR MoClo: Improved MoClo Assembly Standard and New E. Coli Part Library Enable Rapid Combinatorial Design...//doi.org/10.1021/acssynbio.6b00337. Lee, Michael E., et al. “A Highly Characterized Yeast Toolkit for...model organisms and backgrounds, MoClo can be invaluable for more complex editing and screening experiments...

...polymerase I (PolI3M) is capable of mutagenesis in E. Coli, but this approach requires special bacterial...mutations per nucleotide per generation of wild-type E. coli, while only increasing the standard mutation...be used to map protein-protein interactions, investigate non-coding segments of the genome, or engineer...

...and strategies for their projects. References 1. L. E. Woodard and M. H. Wilson, “piggyBac-ing models and...Biol., Apr. 2016. PubMed PMID: 27075976. 4.  X. Li, E. R. Burnight, A. L. Cooney, N. Malani, T. Brady, J.... Menon, G. S. Parker, S. J. Qualls, B. M. Lewis, E. Ke, C. T. Dargitz, R. Wright, A. Khanna, F. H. Gage... likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on the piggyBac™... Grégoire and M. Kmita, “Recombination between inverted loxP sites is cytotoxic for proliferating cells...

...infections. The Cas3 enzymes target several strains of E. coli and lethally chop their genomes into many, many...Hussein M, Molina MA, Berkhout B, Herrera-Carrillo E. A CRISPR-Cas Cure for HIV/AIDS. Int J Mol Sci. 2023...strategies for CRISPR therapies. Systemic therapies involve CRISPR-Cas delivery directly to the patient. Patient-derived...returned to the patient. Finally, allogenic therapies involve editing healthy donor-derived cells and delivering... treatment strategies for these disorders have involved turning on fetal hemoglobin to compensate for ...returning them to the patient. The first human trial involving CRISPR Cas9 for these diseases was sponsored by...evolving, and many of the challenges with therapies involve patient safety, effectiveness, and delivery. Patient...

...345, 626–628 (2014). PubMed. Gantz, V. M. & Bier, E. The mutagenic chain reaction: A method for converting... doi:10.1126/science.aaa5945. PubMed. DiCarlo, J. E., Chavez, A., Dietz, S. L., Esvelt, K. M. & Church...damage public trust in scientists, experiments involving potential gene drives should be conducted with...strategies are available? Molecular confinement involves building gene drives that can spread through populations... been tested in yeast5. Ecological confinement involves performing experiments in a geographic area where...relevant mosquito species. Reproductive confinement involves working with laboratory organisms that can't reproduce...publications. Thank you to our guest blogger! An inventor of technologies that harness evolution, Kevin ...

... In the late nineties, this was developed into an E. coli system, with the noncanonical amino acid in ...expression of phosphoserine-including proteins in E. coli (Zhu et al., 2022). It uses the TAG codon for...components, meaning that the final protocol may involve a sensitive balance of trade-offs. This balance...

...On the 11th day of CRISPR, Addgene gave to me: Cas-E-le-ven! (Cas11)🎵 On the 10th day of CRISPR, Addgene... the 12th day of CRISPR, Addgene gave to me: Noninvasive assessment of gut function using transcriptional...

... were used to express the necessary components in E. coli (Klompe et al., 2019). Recently, the lab introduced...temperatures. Data adapted from Vo et al., 2020. In E. coli, the new streamlined VchINT system was shown...programmability: depending on the transposases involved, they insert either randomly, or at fixed genomic... L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon system. (B) ... a streamlined expression approach (Fig. 2A), involving two-plasmid or single-plasmid systems (Vo et al...insertions are not currently possible, unlike methods involving homologous recombination. Additionally, these ...

...Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L...678 . https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine...and it may also be aligned to plasmids in our inventory containing the suspected contaminating sequence...

...us your plasmid information in spreadsheet format, e-mail us at  [email protected] Special cases Please...can distribute your bacterial strains if they: are E. coli that can be used under BSL1 conditions were...allow you to produce large amounts of your insert in E. coli, then this is a Bacterial Expression vector....deposit, associate the plasmids with a Principal Investigator (PI), provide us with your shipping address ...

...projects underway in the Ellis lab including yeast and E. coli engineering, microbial cooperation, and antibiotic...team also investigates bacteria and extremophiles for biotechnology and has been directly involved in supervising... through Open Access. Chapter 7/Outro: Tom’s Involvement with iGEM and Addgene  Many thanks to our guest... He returned to academic research in 2005 to investigate synthetic biology at Boston University, working...

...screens." bioRxiv (2018): 356626. 6. Tanenbaum, Marvin E., et al. "A protein-tagging system for signal amplification...Resources Read about CRISPRi and CRISPRa from the NIH E-newsletter ...-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to...

...to cancer.   References and resources Milling, L. E., Markson, S. C., Tjokrosurjo, Q., Derosia, N. M.,...pRDA_526 vector to knock out 30 genes putatively involved in CD8+ T cell responses to cancer. The screen...

... UBMTA Chapter 4 - Developing Addgene as a modern e-commerce company and establishing credibility Chapter...Operating Officer. Prior to Addgene, Kenneth was an investment banking analyst at Credit Suisse’s Technology...

...Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire E, Morin X, Livet J. Neuron. 2014...2.1 cleverly combines Cre-mediated deletion and inversion. The Brainbow-2.1 construct can express one of... or M-CFP.) The construct contains two tandem invertible segments, each with two fluorescent protein coding...color possibilities. The remaining cassette can invert as long as Cre remains expressed; once Cre is removed...recombination is required to prevent constant inversion of the construct. Sanes and Lichtman used pronuclear...