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  1. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...the group mentoring format Adding peer advice increases the diversity of input and perspectives Provides...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan...

  2. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...delivery into recipients by conjugation. The labs screened for the host-range of the plasmids and suitability...of cells containing the particle exponentially decreases over time and the number of elapsed generations...

  3. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...including rapidly depleting a protein-of-interest, increasing target selectivity, overcoming resistance to ...molecule binders. To overcome this limitation, we created a generalizable tag-based strategy, which we refer...

  5. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...excitation light less efficiently. Similarly, decreasing the power of the excitation light reduces the... excitation/emission cycles per unit of time, decreasing the likelihood that a fluorophore will bleach...

  6. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...In both cases, red fluorescence in the vacuole increased with longer exposure to autophagy-inducing conditions...

  7. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all...details. Or challenge yourself to think of more creative solutions. Ultimately, problem solving is less...

  8. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...function, however longer histidine strings can increase that risk.    Number of residues Deciding... very low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended...

  9. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...Light chain. C = Constant domain. H = Heavy chain. Created with biorender.com     The modularity of antibodies...antibody and common antigen binding fragments. Created with biorender.com.   Why Fabs?  Antibody fragments...

  10. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... or phosphate molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent...surprisingly well-tolerated by the Cas9 protein and can increase on-target efficiency. In the same vein, locked...

  11. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...germane cognitive load refers to the work put into creating a permanent store of knowledge (a schema).”  In...images, noises and music, spoken words, and any onscreen text. Be aware of the effort the audience is expending...

  12. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...Most coaches have websites you can review and most create useful online content in the form of blogs or articles...us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched...

  13. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength...for funding improves the associated skill sets. Increased experience in communicating and motivating research...

  15. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ... off-target effects Prolonged Cas activity can increase the chance of off-target editing particularly ...Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem...

  16. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...participants can identify you. You may also wish to make creative use of a footer in your poster. This is a section...scientific poster, you shouldn’t hesitate to be creative. After all, your poster is a representation of...

  17. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins...cells (Zhang et al., 2015). Recently the Ward lab created a tool-kit to expand upon these original tools ...

  18. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ..., use a broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding...cross-adsorption process filters out off-target antibodies increasing specificity and reducing cross-reactivity. For...

  19. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...for NRN PAMs and to a lesser extent NYN. This increases the flexibility of gRNA placement significantly...NYN PAMs; it just isn’t always ideal. But the increased flexibility helps even when it’s not an ideal ...

  20. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...
Showing: 561 - 580 of 775 results