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We narrowed to 791 results for: Cre

Showing: 521 - 540 of 791 results

  1. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ... describes an ingenious but simple technique to screen for proteins that could be useful for a wide variety...event gives me the opportunity to dress up as Mr. Incredible, an added bonus. You can find the rest of our...

  2. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...can almost be boiled down to one, key concept: Creating a culture of excellent, effective communication...practice and training. This is important because it creates a culture of engagement in the issues at hand and...

  4. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ... are we! This expansion will give researchers increased flexibility when planning their research and expanded...This split allows us to provide researchers with increased choice and flexibility; while ensuring we can ...

  5. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ... to GRABNE, and having both colors allows for increased options for multiplexing in experiments, including...both nLightG and nLightR showing significantly increased selectivity for norepinephrine over dopamine. ...

  7. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...ahold of your destinies” and also suggested some concrete actions that can be taken now to solve some of...the possible science careers. She advocated for increasing the value and priority put on good mentoring ...

  9. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...submicromolar) DA affinity and high-magnitude responses (increases in fluorescence) at low concentrations (e.g., ...The GRABDA dopamine sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: ...

  10. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...operons from two different bacteria, Bourdeau et al created two constructs optimized for expression in E. coli... and have already developed a high-throughput screening method for serial acoustic collapse to aid in ...

  11. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...DNA-protein interactions, the reliance on fixation does increase the chance of false-positives, as proteins may...RUN doesn’t require fixation and significantly decreases background noise, reducing the read depth necessary...

  12. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ... to help you do better research. CRISPR is an incredibly fast-moving field, and we want to make it easy...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality...

  14. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  ... mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al., 2021....

  16. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Scientists continue to engineer opsins to operate at increasingly faster kinetics. The opsin ChETA, for example...regions of a neuron. Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the...

  17. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...accurately reflect our culture and outlook: Addgene increases productivity by providing world-wide access to...leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters a culture...

  18. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...leader’s job to provide the driving inspiration, to create a productive culture and to correct systemic problems...try operating in a different role?  I think the secret sauce can be summed up in one word – initiative...

  19. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...your applications and experimental design. You can create a new strain with a disrupted gene by transforming...
Showing: 521 - 540 of 791 results