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We narrowed to 803 results for: Cre

Showing: 481 - 500 of 803 results

  1. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...this through a variety of activities, including creating educational materials and lesson plans that are...sterilization of women in marginalized communities have created a legacy of distrust between these communities ...communities, including those by researchers hoping to increase community participation in new precision medicine...

  2. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...using the plexWell technology to easily and quickly create Illumina sequencing libraries. From start to finish...ask the depositing laboratory to review the discrepancies. If the depositing laboratory confirms that ...-Cas9 knockout and transcriptional activation screening. Nat Protoc 12:828–863 . https://doi.org/10.1038...

  4. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...growth or decline of interest in a field over time. Creating an alert for a search term helps automate your...is not a traditional search engine. Instead, you create your own research feed by entering keywords, concepts...new papers that contain these terms. You can also create intersects in your feed which means only papers...

  5. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...Mammalian expression plasmids are primarily used to create mRNA and the commonly used mammalian terminators...below) releases the mRNA from the polymerase and creates a free 3' end which is now available for polyadenylation...Internal Polyadenylation Signal Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes...

  6. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...Victoria, CA Over the decades science has become increasingly restricted to academic and industrial labs, ...research. This interest manifested itself in the creation of Hackerspaces and Makerspaces back in 2007, ... make accounts with  Addgene. We now have the incredible CRISPR/cas9 tools at our disposal to to modify...

  7. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...disulfide bridges, glycosylation and acetylation to create functional, stable proteins. Protein expression...scientists have taken advantage of the natural secretion of biochemicals and proteins through plant roots... Tagging recombinant proteins with a naturally secreted plant peptide allows for easier access and purification...

  8. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...as 30% of all cancers, especially those of the pancreas and colon. Despite the prevalence of Ras mutations...he or she can use to study it (as seen in the screenshot below). Currently, the page includes interactive... Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used...

  9. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...parallel because the risk of accidental errors increases. In addition to human error, fragments of DNA ...to the reference sequence of the plasmid used to create the viral prep. In a clean viral prep, like AAV...control process! The images in this blog post were created using Snapgene and Geneious software. References...

  10. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    .... At Addgene, we use blastn to identify any discrepancies in Sanger sequences, such as mismatches, deletions...determine the potential effect of any nucleotide discrepancies. The blastp and blastx programs are optimized...plasmids instead! All BLAST images are modified screen shots from the NCBI BLAST website. Additional Resources...

  11. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...a double-edged sword. An antibody’s large size creates more distance between the target antigen and the...also allows for better tissue penetration and decreases the distance between a fluorescent tag and the...HCab antibodies from its serum, and meticulously screening, optimizing, and characterizing several anti-mouse...

  12. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...overexpression of protein fusions to gene-edited cells can decrease the number of fluorophores, compromising the photon...improved fluorophores are making chemical dyes increasingly attractive and accessible to cell biologists—and...fluorogenicity, photoactivation etc.—can further increase the photon budget and allow us to push further...

  13. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...expense and requirement for lab equipment. Plus they create plastic waste. Kits can also have the annoying ...and filters can be stacked together in series to increase binding capacity. Figure 1: Purify DNA using...Additionally, while commercial plasmid prep kits come in discreet sizes (mini, midi, maxi, giga) which requires ...

  14. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...just might personally hand your credentials to HR, greatly increasing your chances of getting a phone ...can use to better prepare for your career, and increase your chances of landing that next coveted job!...

  15. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...researchers in the field. Finally, you can sound credible and cool when you casually mention “Haery et al... al., showed that MYC expression was increased…” when discussing the review topic with your peers. Start...will also paraphrase what I have heard many other creative people say about writing: you don’t know what ...

  16. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...prognoses. Survival rates for mesothelioma are creeping upwards, and while most people still die within...world. New mesothelioma cases could be greatly decreased through a ban on asbestos. Nonprofits like the...Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional Resources on Addgene.org ...

  17. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...that can function in more than one host species. Created with BioRender.com. How are shuttle vectors...viral oris to drive amplification, which helps increase cellular uptake, but this approach requires compatible...eukaryotes. Conclusion I still think it’s pretty incredible that my cells “speak” the same molecular language...

  18. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...proteins cooperatively can separate the DNA strands. Created with BioRender.com. Once DnaA breaks the local...helicases to continue unwinding the strands. This creates two replication forks that move in opposite directions...pseudoknot from folding, blocking replication. Created with BioRender.com. Plasmids under stringent...

  19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...made use of mazF as a counterselectable marker to create unmarked genetic modifications in the yeast Pichia...counter selection in one plasmid, they were able to create an efficient cloning tool. The plasmid pKSCTMF ...Morin and James Kaper used the hok/sok system to create strains that can stably express antigens in sufficient...

  20. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...expression transposases leading to increased integration frequency. (C) Increasing rounds of transfer on transposition-inducing...chromosomal integration of cargo without using screening markers. Capability to construct a strain library...
Showing: 481 - 500 of 803 results