We narrowed to 805 results for: Cre

...and their GPCR binding partner, Bryan Roth’s lab created an open source resource called PRESTO-TANGO (parallel...parallel receptorome expression and screening via transcriptional output - TANGO) (Kroeze et al., 2015...are ideal candidates for use in chemogenetics. To create chemogenetics receptors, GPCRs were first mutated...

...well-characterized. During this time, we’ve also created a variety of educational resources in an effort...understanding of Addgene’s customer’s needs. Categories We created a categorization of our information in order to...for promoter and serotype, among others. We also created a normalization mechanism to “collapse” variations...

...emitted yellow-green light and exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient...injected intravenously with coelenterazine, with increased sensitivity and faster imaging compared to previous... reporter genes for endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent ...

...production. Finally, we are blessed to have an incredibly supportive Board of Directors at Addgene. Our...leadership of Addgene’s co-founder, Melina Fan, we created a new team to push this project forward. The viral...you to the Addgenies who went the extra mile to create this new service. We knew the scientists were waiting...

...under cold temperatures, and frigid conditions decrease the potential for microbial contamination. Additionally...Acclimatisation To resolve this impasse, our team decided to create a hybrid system where we could incorporate cold-tolerance...The event also featured a seminar on “Tools to increase scientific reproducibility” by Benoit Giquel, ...

...sequence (pSpCas9(BB)). The 2A-GFP allows for screening of transfected cells. Find pSpCas9(BB)-2A-GFP ...virus, making it ideal for CRISPR pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked...site, a pair of annealed oligos can be cloned to create a chimeric guide RNA. Find pX330-U6-Chimeric_BB-CBh-hSpCas9...

...mutations through homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary...that enhance pol III transcription, potentially increasing the levels of gRNA expression. Since tRNAs are... of multiple gRNAs targeting a single gene can increase the likelihood of gene knockout, or multiple gRNAs...

...funding and time. While institutions that focus on creative problem solving like the Howard Hughes Medical...plant research, more initiatives are needed to increase the body of knowledge. Organizations like Open...research and effectively carry out their mission of creating new forums and opportunities for open innovation...

... the world of science publishing, and an ever increasing number of journals and publishers to choose from...them needing a subscription to the journal. An increasing number of funding bodies insist that the research...is beneficial for both the referees (as they get credit for their time and effort) and the scientific community...

...publishing has a number of drawbacks, especially the increasingly growing time from submission to publication....for their efforts, inspire future research, and increase their impact. Open access and transparency Another...journal publication or an updated preprint, further increasing their findability. Now, go out there and finish...

...electric! After attending this meeting I had an increased appreciation for the role of ion channels and ... the entire worm during regeneration, the worm creates two heads. Subsequent cutting results in a two-...pHLuorin fluorescent protein. This GEVI features increased brightness and improved plasma membrane specificity...

... viewed your profile.”  The latter is much less creepy. If we’ve met before, I’d be much more likely to...borders on my first rule of networking--”If it feels creepy, don’t do it.” It’s just not a useful way to build...rules of good resume writing. Include examples of concrete accomplishments in both the technical and “plus...

...cells in the microenvironment. This results in increased expression of transforming growth factor-β signaling...signaling, possibly inducing the secretion of growth promoting proteins, such as human growth factor, vascular... (Kogure et al., 2011) believes that miRNA may decrease the expression of TAK 1, an enzyme that controls...

...figure out how to optimize protocols for in vivo screening with lentiviral pooled shRNA libraries. Later,...reagents.” Here at Addgene, we’re excited to see the creative new ways you put the ClonTracer library to use... Blog Learn about the Sabatini and Wood Drug Screening Kit Find Out How to Best Utilize Our Cancer Resource...

...Figure 1: Common functions of natural plasmids. Created with BioRender.com. Resistance (R) plasmids...resistance genes to help cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids...years, and new classifications are still being created as whole-genome sequencing becomes more relevant...

...  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance By Hridaya Patel...authors used a multispecies approach to generate and screen a library of AAV9 variants (Chuapoco et al., 2023... Figure 3: Overview of library generation and screening that led to identification and characterization...

...this through a variety of activities, including creating educational materials and lesson plans that are...sterilization of women in marginalized communities have created a legacy of distrust between these communities ...communities, including those by researchers hoping to increase community participation in new precision medicine...

...response to blue light. This tool, CRY2clust, was created by adding a 9-residue peptide to the C-terminus...regions. To overcome this issue, Shemesh et. al. created a soma-targeted derivative of the opsin CoChR, ...within the cell. Their Fluorescence fLuctuation INcrease by Contact (FLINC)-based biosensors measure fluctuations...

...specially engineered sgRNAs to increase transcription. This is done through creating a dCas9/VP64 fusion protein...CRISPRa systems recruit transcription factors to increase gene expression. CRISPRa methods vary in their...

...activation or repression, transgenic mouse line creation, and in vivo gene editing of mice, with the potential...cationic polymer. Polybrene has long been used to increase retroviral transduction efficiency by neutralizing...Nanoblades CRISPR has also been extensively used to create transgenic mice by microinjecting zygotes with ...