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Showing: 441 - 460 of 726 results

  1. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...antibody and a ≥100-fold increase in signal over intrinsic fluorescence. Increased signal is likely due to...stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible with most...

  2. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...channels that are responsible for maintaining the nutrient and ionic balance of the cells,” says Dr Sangeetha...keratinocytes, which release inflammatory mediators that trigger the activation of dendritic cells, macrophages,...

  3. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular... depend on fluorescence intensity or protein distribution. Therefore, photostability measurements can ...

  4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...Greek for “self-eating”) is induced by a lack of nutrients and targets cytosol and organelles to the vacuole... have fused with the vacuole or a lysosome are trickier to identify because their contents can be at various...

  5. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody...support team, is a safer bet than one from a large distributor that may not have direct experience with the ...

  6. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...These modifications result in conformationally restricted RNA which improves mismatch discrimination and... specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce the innate immune response...

  8. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently.../19 vectors and demonstrating the concept of restriction enzyme-mediated disruption of ccdB, leading to...

  9. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Alan Wong. The complexity of biological systems can hinder...iterative one-pot reactions with straightforward restriction digestion and ligation steps to build barcoded...

  10. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    .... These plasmids have reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other... after reverse transcription (RT-LAMP) and a colorimetric readout that can be completed in just 45 minutes...

  11. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...laboratory of Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will... function in receiver cells. There are over 40 trillion cells that make up the human body and G-baToN ...

  12. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...photostable YFP to date. As such, mGold is expected to contribute to experiments that require high photostability...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth...

  14. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...efficiency and any unpaired fluorescent proteins can contribute additional noise to the measurement. If the distance... FRET experiments are expected to continue to contribute to our understanding of diseases and even assist...

  16. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...green, blue, red, and far-red. There is also a ratiometric GECO sensor (GEX), where one wavelength changes... working with a stably integrated biosensor. Ratiometric measurements with certain single FPs (such as...

  17. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...although both are of course preferred! When trying to strike that balance, you may wonder: how close is close...significant amount of flexibility compared to a strict single-compatibility sequence like NGG. Nonetheless...

  18. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...References and resources References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application...(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary...

  19. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ... to prep large quantities of DNA because some countries just can’t receive bacteria through the mail due..., if you have any great lab automation tips and tricks, be sure to share them in the comments section....

  20. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...plays an important role in RNA processing and contributes to variability in RNA half-life, and ultimately... Rho-independent termination is also known as intrinsic termination, and it relies on the formation of...
Showing: 441 - 460 of 726 results