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  1. Distribution to Industry

    Type
    Collection
    ... Distribution to Industry Featured Collections Search Plasmids...validation and drug design Antibodies Addgene distributes ready-to-use recombinant antibodies made from...Addgene’s deposit agreements allow Addgene to distribute plasmids on behalf of the depositing institutions...the language which we are allowed to use for distribution. Please see the Sample Agreements on our Technology...order will be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection is ...

  2. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Anti-Beta4-spectrin [N393/2R] Beta4-spectrin Human Mouse IgG2a 177542 Anti-Beta4-spectrin [N393/76R] ...Species Host Species Isotype 114473 Anti-TRIP8b (exon 4) [N212/3R] TRIP8b (exon 4) Rat Mouse IgG2a 114474 Anti-Clcn4...SUR1 Rat Mouse IgG2a 114557 Anti-TRIP8b (constant) [N269/73R] TRIP8b (constant) Rat Mouse IgG2a 114558...SynCAM4 Mouse Mouse IgG2a 114563 Anti-TRIP8b (exon 1a/5) [N291C/22R] TRIP8b (exon 1a/5) Mouse Mouse IgG2a 128612...IgG2a 149455 Anti-MMP9 matrix metalloproteinase-9 precursor [L51/82R] MMP9 matrix metalloproteinase-9 precursor...Ca2+ channel Rat Mouse IgG2a 164154 Anti-TRIP8b [N212/7R] TRIP8b Rat Mouse IgG2a 164155 Anti-GABA(A)R, alpha6...LRP4 Mouse Mouse IgG2a 177514 Anti-TRIP8b (constant) [N212/7R] TRIP8b (constant) Rat Mouse IgG2a 177515...

  3. DNA Service - Cloning Grade DNA

    Type
    Collection
    ... Concentration 100 ng/µl (± 5ng/µl) Buffer 10 mM Tris-Cl, pH 8.5 Price Please log in to your account and...

  4. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ... be for efficient recombination? The most we've tried to insert so far has been 1kb. We used homology ...template after performing gDNA purification and have tried conditions with 5% DMSO or without 5% DMSO. My genomic...template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) ( see Figure...

  5. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ... sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for in vitro as well as in vivo...PMID: 24191859 Ye, et al. 2015. Safety and Biodistribution Evaluation in Cynomolgus Macaques of rAAV2tYF-CB-hRS1...Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Mol Ther Nucleic Acids . Sep...

  6. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Run samples on 2% agarose gel at 10 V/cm using 1x Tris-acetate-EDTA (TAE) buffer. Examine samples for the...step 3.1.4 ), 5.0 μl restriction enzyme buffer (10x), 4.0 μl (20 U) BbsI restriction enzyme (5,000 U/ml)...for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 ). Add a G nucleotide after ...-well PCR plate using a multichannel pipette. Centrifuge PCR plate at 400 x g for 5 min and remove supernatant...

  7. Genetic Code Expansion

    Type
    Collection
    ... Plant TAG Chang Liu 85484 pDule-tfmF A65V S158A tri-fluoromethyl-phenylalanine synthetase M. jannaschii... Mehl 163915 pRSF-ChPylTMSK ChPylTMSK N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK) Bacterial...Huber 177311 pRSF-G1TFAKRS M. archaeon N6-(trifluoroacetyl)-L-lysine (TFA-Lys) Bacterial TAG Thomas Huber...200226 pMega-MaFRSA PylRS M. alvus meta-trifluoromethylphenylalanine Bacterial TAG Alanna Schepartz 201922...

  8. Brain Armamentarium

    Type
    Collection
    ...BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru...BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru...protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru...BGHpA (Alias: CN4361) Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs...BGHpA (Alias: CN4360) Expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs) ...BGHpA (Alias: CN4035) Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs...BGHpA (Alias: CN4033) Expression of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs) ...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...biosensor for cyclic-di-GMP measurement Asymmetrical distribution of the second messenger c-di-GMP upon ...redox-sensitive ratiometric sensor roGFP for mitochondria or cytosol Hypoxia triggers subcellular compartmental...depdent on Cre, Flp, or Vcre Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of...bioRxiv 2023.10.12.562058. Robert Campbell Calcium Ratiometric calcium biosensors from nested reporters of green... green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested cassette of green...anisotropy Cameleon calcium sensors Single-color, ratiometric biosensors for detecting signaling activities... Cre- or Flp-dependent) Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of...

  10. Immunology Research Plasmids and Resources

    Type
    Collection
    ...KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9 TNFRSF10C tumor necrosis factor... to a specific trigger. Symbol Name Synonyms ACVR1B activin A receptor, type IB ACTRIB, ACVRLK4, ALK4,... A receptor, type IIA ACTRII, ACVR2 ACVR2B activin A receptor, type IIB ACTRIIB, ActR-IIB, MGC116908 ACVRL1...secretagogue receptor - GIP gastric inhibitory polypeptide - GIPR gastric inhibitory polypeptide receptor...FMRFAL NPPA natriuretic peptide precursor A ANF, ANP, ATFB6, CDD-ANF, PND NPPB natriuretic peptide precursor...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CMV ALS Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS Trina Schroer...H1 ALS Patrick Aebischer 10881 pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch SOD1 H1 ALS Patrick Aebischer...CMV Parkinson's Patrick Aebischer 36070 pAAV asyn S87A/S129A SNCA CMV Parkinson's Patrick Aebischer 36071...EGFP OPTN GFP CMV ALS Beatrice Yue 68839 pOPTN1-209-EGFP OPTN GFP CMV ALS Beatrice Yue 68840 pOPTN1-424...EGFP OPTN GFP CMV ALS Beatrice Yue 68841 pOPTN217-577-EGFP OPTN GFP CMV ALS Beatrice Yue 68842 pOPTN217-...EGFP OPTN GFP CMV ALS Beatrice Yue 68843 pOPTN425-577-EGFP OPTN GFP CMV ALS Beatrice Yue 68846 pR96L-EGFP...pR96L-EGFP OPTN GFP CMV ALS Beatrice Yue 68847 pD474N-EGFP OPTN GFP CMV ALS Beatrice Yue 68848 pE478G-EGFP OPTN...

  12. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1 - Mammalian...Expression mAmetrine 406 526 26 6 0.8 hr Monomer (A206K) pBad-mAmetrine - Bacterial Expression mAmetrine-N1 -...- Mammalian Expression mCitrine 516 529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C)... Expression pCS2+8CmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus...Mammalian Expression mCitrine-C1 - Mammalian Expression mCitrine-pBAD - Bacterial Expression YPet 517 530 80 ... - Mammalian Expression mAmetrine-C1 - Mammalian Expression LSSmOrange 437 572 23 5.7 2.3 hr Monomer pLSSmOrange-C1...Mammalian Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many...

  13. Chemogenetics AAV Preps

    Type
    Collection
    ...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8 Roth...-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Roth ...-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Roth ...pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine none 2, 8 Roth 50476 ...pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine rM3D(Gs) - Activation HA fusion and IRES mCitrine none 5 Roth 50478 pAAV-GFAP-hM3D...element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent...

  14. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...-CDS issues. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI Restriction Sites to Avoid: NotI For...Coli genome. Prohibited Restriction Sites: EcoRI, SpeI, NheI, PstI Restriction Sites to Avoid: PvuII, ... use of assembly standards – combinations of restriction sites which preserve a standard prefix and suffix...assembly standards most commonly used by part registries, including the specific prefix and suffix sequences... reverse complimented for primer design. The restriction enzymes used for assembly are the prohibited ... Employs high-efficiency enzymes. Prohibited Restriction Sites: EcoRI, BglII, BamHI, XhoI For more info...giving in-frame scar encoding Thr-Ala. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI For more info,...

  15. Mammalian RNAi Tools

    Type
    Collection
    ...easily visualize products from an AgeI/EcoRI restriction digest) David Root 10879 pLKO.1 ‐ TRC control...shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono 11779 pLVCT-rtTR-KRAB...shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick Aebischer and Didier Trono 11644 pLVET-tTR-KRAB...shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono 11642 pLVPT-tTR-KRAB...shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono 11652 pLVPT-rtTR-KRAB...shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick Aebischer and Didier Trono 11651 pLVUT-tTR-KRAB...shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono 11648 pLVPRT-tTR-KRAB...

  16. Rett Syndrome

    Type
    Collection
    ...opens in a new window) ClinicalTrials.gov - a list of Rett syndrome clinical trials (Link opens in a new...in a new window) PMID: 17289941 2010 - Allyson Muotri's lab develops the first iPSC model for Rett syndrome...: 26944080 (Link opens in a new window) Alysson Muotri Q83X C247T Q83X M Fibroblasts & iPSC (Link opens...: 26944080 (Link opens in a new window) Alysson Muotri c.806delG 806delG G269Afs*20 M Fibroblasts & iPSC...new window) HRV Metrics Toolbox - algorithms for data analysis to calculate heart rate variability (Link...in Australia: a review of the epidemiology. J Pediatric . 148(3):347-352. (Link opens in a new window)...Return to top Help us improve this resource by contributing your plasmids! To get started with your deposit...

  17. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Dyche Mullins 41147 pEGFP Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg 41151 ...KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL tripeptide, peroxisome transport signal dL5 FAP; mCerulean3...41151 pEGFP Cep170 C-term Centrioles (dependent on cell cycle) Cep170 EGFP Erich Nigg 41163 pEGFP PICH Centromeres... mCherry Gia Voeltz 89472 GFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 GFP Ken-Ichi Takemaru ...Takemaru 89770 pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base Chibby1 EGFP Ken-Ichi Takemaru 118084 pLenti-EB1...EB1 EGFP Ken-Ichi Takemaru 122871 GFP-hCCDC11 Centriolar satellites CCDC11 EGFP Ken-Ichi Takemaru 54234...-alpha Tubulin Microtubules alpha-tubulin EGFP Patricia Wadsworth 27123 eTC GFP beta-actin full length...

  18. Antibody Production

    Type
    Collection
    ...undergo stringent quality control by Addgene or a trusted quality control partner before distribution. Details...Production Antibody Production Many antibodies distributed by Addgene have been produced in-house by Addgene...contacting [email protected] . Some of the antibodies distributed by Addgene were developed by the Institute for..., antibodies undergo a buffer exchange using centrifugal columns. The final formulation buffer is phosphate-buffered.... Only antibody samples with >90% purity are distributed. Quality Control Each lot of antibody undergoes...

  19. TALEN Guide

    Type
    Collection
    ...fingers that recognize various nucleotide triplets. With some trial and error, ZF arrays are able to recognize...zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer, cutting...their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and interactions... targets are confined to sequences composed of triplets with corresponding zinc fingers, potential targetable...genome are every 500 bp. TAL effectors have some restrictions (for example, the target must start with a T...

  20. Antibody Guide

    Type
    Collection
    ...light orange, that bind to a specific antigen, triggering an immune response. The stem of the Y structure...single antibody signal. Here, HRP is used as a colorimetric, in which the reaction produces a colored product...the amount of enzyme present in each well. In colorimetric assays, protein amount can be determined by ..., lyse, and then collect protein extract by centrifugation or affinity columns. Mix samples with a primary...immobilized on agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic... your samples using permeabilization, antigen retrieval, and/or sectioning. Block to reduce non-specific...tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule may be a...
Showing: 1 - 20 of 93 results