... Distribution to Industry Featured Collections Search Plasmids...validation and drug design Antibodies Addgene distributes ready-to-use recombinant antibodies made from...Addgene’s deposit agreements allow Addgene to distribute plasmids on behalf of the depositing institutions...the language which we are allowed to use for distribution. Please see the Sample Agreements on our Technology...order will be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection is ...

... Anti-Beta4-spectrin [N393/2R] Beta4-spectrin Human Mouse IgG2a 177542 Anti-Beta4-spectrin [N393/76R] ...Species Host Species Isotype 114473 Anti-TRIP8b (exon 4) [N212/3R] TRIP8b (exon 4) Rat Mouse IgG2a 114474 Anti-Clcn4...SUR1 Rat Mouse IgG2a 114557 Anti-TRIP8b (constant) [N269/73R] TRIP8b (constant) Rat Mouse IgG2a 114558...SynCAM4 Mouse Mouse IgG2a 114563 Anti-TRIP8b (exon 1a/5) [N291C/22R] TRIP8b (exon 1a/5) Mouse Mouse IgG2a 128612...IgG2a 149455 Anti-MMP9 matrix metalloproteinase-9 precursor [L51/82R] MMP9 matrix metalloproteinase-9 precursor...Ca2+ channel Rat Mouse IgG2a 164154 Anti-TRIP8b [N212/7R] TRIP8b Rat Mouse IgG2a 164155 Anti-GABA(A)R, alpha6...LRP4 Mouse Mouse IgG2a 177514 Anti-TRIP8b (constant) [N212/7R] TRIP8b (constant) Rat Mouse IgG2a 177515...

... Concentration 100 ng/µl (± 5ng/µl) Buffer 10 mM Tris-Cl, pH 8.5 Price Please log in to your account and...

... be for efficient recombination? The most we've tried to insert so far has been 1kb. We used homology ...template after performing gDNA purification and have tried conditions with 5% DMSO or without 5% DMSO. My genomic...template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) ( see Figure...

... sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for in vitro as well as in vivo...PMID: 24191859 Ye, et al. 2015. Safety and Biodistribution Evaluation in Cynomolgus Macaques of rAAV2tYF-CB-hRS1...Engineered AAV Capsids Improve Transduction and Volumetric Spread in the CNS. Mol Ther Nucleic Acids . Sep...

...Run samples on 2% agarose gel at 10 V/cm using 1x Tris-acetate-EDTA (TAE) buffer. Examine samples for the...step 3.1.4 ), 5.0 μl restriction enzyme buffer (10x), 4.0 μl (20 U) BbsI restriction enzyme (5,000 U/ml)...for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 ). Add a G nucleotide after ...-well PCR plate using a multichannel pipette. Centrifuge PCR plate at 400 x g for 5 min and remove supernatant...

...DNA elution choices: TE, Tris buffer, or water Figure 2: Tris-EDTA, Tris, or water? The buffer you...protocols that recommend eluting in water, Tris-EDTA (TE), just Tris buffer, or some other variations. Does... affect your downstream experiment. TE (10 mM Tris-HCl,1 mM EDTA, pH 8.0) buffer is the best buffer...the stability of your preparation for a long time. Tris buffer controls the pH, while the EDTA chelates ... enzymatic reactions, a valid alternative can be Tris buffer (with no EDTA) or water. These are two of... DNA through their kit. The advantage of using a Tris buffer is that it allows better control of the pH...for a short period, and there are indications that Tris buffer is better than water in these conditions....

...When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone... of how to do perform restriction digests.   Overview of the restriction cloning process. Both ...backbone will contain a variety of restriction enzyme cut sites (restriction sites) downstream of the promoter...DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently...sequence of interest (green, insert) are cut with restriction enzymes to generate compatible overhangs that...backbone covalent.  Before beginning the restriction digest and ligation process, you should carefully...these both must have compatible cut sites for restriction enzymes that allow your insert to be placed into...

...you to all who have contributed to the Addgene blog this year! Your contributions have helped researchers...: Erik Lee SnappSharing is Caring, But How to Distribute Open Hardware? Author: Benedict Diederich Course-Based...

...Thirteen: Guest Contributors of 2023 8 Great Depositor Research Stories Interested in contributing to the Addgene...bloggers and subject matter experts who have contributed to our blog in 2024. Thank you to…   Marty LaFleur...Kits We are very grateful for each and every contributor to the blog. Thank you! More resources on the...

...chromosome and for stringently controlled plasmids. Binding of IHF (green) redistributes DnaA (red) from ...chromosomal DNA.  Plasmids are said to be under stringent control when they depend on the host cell’s initiation...consider the replication mechanism of plasmids under stringent control, such as pSC101. DNA replication and regulation...appropriate time—for example, the cell must have enough nutrients available to complete the entire round of replication...assists with the process: it bends the DNA to redistribute the DnaA from high-affinity boxes to low-affinity...replication. A variety of other proteins also contribute to this regulation in different ways: methylating...DNA replication and regulation Plasmids must also strike a balance: replicating too slowly will eventually...

...email [email protected] if you would like to contribute to the catalog. The Catalog Will Start With Neuroscience...antibodies available at launch were created by James Trimmer, PhD, of the University of California Davis School... Figure 1: Antibody against PSD-95 from the Trimmer Lab. Information about the antibody, the target...Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant...

...Have you ever tried digesting with XbaI or ClaI restriction enzymes and gotten unusual or unexpected ...methylation may affect your restriction digests. Why methylate? Restriction enoducleases are so ubiquitous...It turns out that restriction enzymes are one half of naturally occurring restriction modification systems... from the restriction modification systems, yet can still affect whether certain restriction enzymes can...associated with any restriction modification systems, their sequences may overlap with restriction sites, inhibiting...sequences to prevent them from being degraded by restriction endonucleases. A given prokaryote typically has... has genes encoding one or a few restriction modification systems containing methyltransferases that add...

...code, find and fix security-critical bugs, and contribute to constant updates of the software. In hardware...community to regularly replicate designs worldwide. Distributing hardware via globally established makerspace...

... Ingredient Concentration or % Purpose Tris HCl 62.5 mM Buffer SDS 2% (w/v) Detergent...buffer such as PBS or TBST. Stringent stripping The stringent stripping solution uses heat and detergent...Biosystems offer pre-made stripping solutions or kits. Mild stripping The mild stripping solution uses low pH...lose by trying to strip and reprobe. In this case, we recommend using mild stripping solution (see below...with the antibodies, especially with more stringent stripping methods. If you are planning to detect multiple...later, strip it thoroughly first. Then continue on with your western blot as normal! Stripping your membrane... to strip blots, and the best choice will depend on your specific experiment. Remember, stripping your...

...from the bioelectricity field In addition to sharing tools to study bioelectricity, bioelectric researchers...Developmental Bioelectricity symposium’s “What is Bioelectricity?” Journal of Bioelectricity ...of biology known as bioelectricity is revealing the important role of bioelectrical signaling mediated ...first introduced to bioelectricity when I attended the Developmental Bioelectricity satellite symposium... these slower-changing bioelectric states in living cells. Since bioelectrical signals are controlled ...Michael Levin. "The body electric 2.0: recent advances in developmental bioelectricity for regenerative and...  All living cells with a membrane possess an electrical state. This state, known as the resting potential...

... technologies distributed through Addgene over our first 10 years. 10 most distributed plasmid technologies...technology, but pSV-PGC1 was Addgene's first distributed plasmid and was created in the lab of my thesis...Bruce Spiegelman. It was also the only plasmid distributed in our 1st year, 2004, and it still gets requested...Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid. A variety of...up with all the new plasmids. So far, we have distributed over 10,000 CRISPR/Cas9 plasmids to more than...

...Researchers in developing countries often find themselves on the front lines of emerging global challenges...Seeding Labs to better serve those in developing countries. Developing an international scientific community...community Seeding Labs, a nonprofit founded in 2007, strives to expand the opportunities and scientific resources...resources available to researchers in developing countries--because scientific talent is everywhere, but ...around the world. As part of our own mission, we strive to help build the international scientific community... are always taking steps to increase plasmid distribution and disseminate knowledge around the globe. .... Addgene’s progress in international distribution As you can see from the chart below, we are making ...

...of how to analyze a restriction digest:     Restriction digest tips and tricks: The following tips ...plasmids. Here, we'll cover restriction digest analysis. Diagnostic restriction digest Diagnostic digests...Diagnostic restriction digests are comprised of 2 separate steps: 1) incubating your DNA with restriction enzymes...to confirm the sequence of all the plasmids we distribute. This method is time-intensive, so we recommend...organization of different features within the plasmid. Restriction analysis can also be used successfully even if...relative sizes of the resulting DNA fragments. Restriction digests are commonly used to confirm the presence...backbone. To do this, you'll use enzymes with restriction sites that flank the insert. You will need to...