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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 798 results for: Cre

Showing: 441 - 460 of 798 results

  1. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...University of Alberta and University of Oregon created mTFP1 by transforming E. coli with a gene library...tetramer-breaking mutations. The library was then screened through successive rounds of mutagenesis for the...filter. This combination results in a 2.6-fold increase in signal relative to mCerulean imaged with a ...

  3. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...R6K, and 15A. Not all origins of replication are created equal. Some will produce many plasmid copies and...because they will compete for the same machinery, creating an unstable and unpredictable environment. As ...plasmids if they contain large inserts or genes that create a toxic product. The E. coli strain Most E....

  4. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ... - not just for fiction! Image credit: Kenneth Rowe, under the creative commons license.    The introduction...is to find one or two roadblocks that sparked creativity, changed the outcome of the project, or illustrates...

  5. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...cassette allowing a researcher to perform blue-white screening after transformation - self joining of the vector...break down the steps needed for TOPO cloning: 1. Create Your PCR Product: Design standard primers (no need...unpaired 3’ ends in your PCR product. If you need to decrease error rate, please use one of these methods to...

  6. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...would rather fund excellent people with mediocre ideas than mediocre people with excellent ideas. I am lucky... your potential as an entrepreneur, not your credentials. If you have an idea, don’t wait.  Go for it....

  7. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...to keep the audience engaged through a computer screen. For the Reproducibility for Everyone Workshop,...author of the poster,” he says.Viewing a poster on a screen is challenging, requiring you to zoom in to different...canceled may be disappointing, but there are many creative ways to adapt and take advantage of the new conference...

  8. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...microvesicles (approx. 40 - 150 nm diameter) that are secreted from the cellular membrane into the culture medium...AAVs (Schiller et al., 2018). Exosomal vesicles secreted from producer cells also contain cellular macromolecules...open new ways of improving gene delivery. They increase the efficiency of transduction, broaden the use...

  9. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ... the stem cell community – a community largely created due to Yamanaka’s seminal work. It didn't take... during his keynote address, appeared almost incredulous that the field has exploded so quickly, citing...explained that it took 50 people nearly 2 years to create the first stem cell line that could be used in ...

  10. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ..., described below, are typically employed when creating stable cell lines and are not used for general...Stable transfection A stable transfection is used to create a population of cells that have fully and successfully...culture media until 24-48 h post transfection when creating stable cell lines. Looking for Mammalian Expression...

  11. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...reaction catalyzed by luciferase, releasing light. Created with BioRender.com. Bioluminescent reporters...Peak emission (nm) Size (kDa) Substrate Cofactors Secreted North American firefly (Photinus pyralis) Natural...cell membrane and deliver the substrate; however, secreted luciferase or alternative substrates can permit...

  12. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ... vector and insert are (separately) digested to create 10–12 base “chewed back” overhangs, they can be...bacteria, which will efficiently repair the plasmid. Created with BioRender.com.   SLIC also opened up a...transformed into bacteria, which repair the plasmid. Created with BioRender.com. How does SLIC compare to...

  13. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...use small, incremental changes to understand what works and what doesn't. By being creative, thoughtful...each step as you complete it. Yes, this can be incredibly tedious, but it also works, so do it anyway.  ...

  14. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...and their GPCR binding partner, Bryan Roth’s lab created an open source resource called PRESTO-TANGO (parallel...parallel receptorome expression and screening via transcriptional output - TANGO) (Kroeze et al., 2015...are ideal candidates for use in chemogenetics. To create chemogenetics receptors, GPCRs were first mutated...

  15. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...under cold temperatures, and frigid conditions decrease the potential for microbial contamination. Additionally...Acclimatisation To resolve this impasse, our team decided to create a hybrid system where we could incorporate cold-tolerance...The event also featured a seminar on “Tools to increase scientific reproducibility” by Benoit Giquel, ...

  16. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...well-characterized. During this time, we’ve also created a variety of educational resources in an effort...understanding of Addgene’s customer’s needs. Categories We created a categorization of our information in order to...for promoter and serotype, among others. We also created a normalization mechanism to “collapse” variations...

  17. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...emitted yellow-green light and exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient...injected intravenously with coelenterazine, with increased sensitivity and faster imaging compared to previous... reporter genes for endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent ...

  18. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...production. Finally, we are blessed to have an incredibly supportive Board of Directors at Addgene. Our...leadership of Addgene’s co-founder, Melina Fan, we created a new team to push this project forward. The viral...you to the Addgenies who went the extra mile to create this new service. We knew the scientists were waiting...

  19. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...sequence (pSpCas9(BB)). The 2A-GFP allows for screening of transfected cells. Find pSpCas9(BB)-2A-GFP ...virus, making it ideal for CRISPR pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked...site, a pair of annealed oligos can be cloned to create a chimeric guide RNA. Find pX330-U6-Chimeric_BB-CBh-hSpCas9...

  20. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...communication skills with training programs, which are increasingly available. But think of this as a jumpstart ...possible outcome of a ComSciCon workshop is the creation of a new science communication initiative that...science communication opportunities in your area, create them.  Seek out collaborations with organizations...
Showing: 441 - 460 of 798 results