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We narrowed to 791 results for: Cre

Showing: 421 - 440 of 791 results

  1. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...response to the cell cycle or other stimuli.   To create a colorful Cas9 toolbox, Ma et al. turned to SpCas9...was fused to a different fluorescent protein to create three colors. The specificity of these orthologs...nucleome a specific priority, as researchers work to increase our understanding of chromatin organization. This...

  2. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...The last trick GeoCas9 has up its sleeve is its increased longevity in human serum. Harrington et al suspected...shown that a cocaine esterase engineered to have increased thermostability also had a longer lifetime in ...Harrington, Lucas, et al. "A thermostable Cas9 with increased lifetime in human plasma." bioRxiv (2017): 138867...

  3. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...your project's potential. Widening your search creates fertile ground for serendipitous encounters that...research and PhD studies can enrich the depth and creativity of your work, while offering mental health benefits...but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking discoveries. So...

  4. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...plasmid analysis tool including editing, annotating, creating maps, and more. This tool is maintained by M. ...University of Utah.   Figure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA. ...repository for Cas enzymes.  Open repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines...

  5. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ... the connection between LEGOs and science more concrete to help further unlock the potential contained...process One major challenge when we started was creating a LEGO stage that resembles a real laboratory.... your experiments to think outside the box, be creative, and put your inner child to work. If you have...

  6. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...provide advice. The society organizes numerous recreational and educational events throughout the year, ...iGEM alumni-industry networking to promote the creation of start ups and biotech spin offs. The Synthetic...Inspiring future scientists At Addgene, we’re incredibly glad to see these biological societies engaging...

  8. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ... these meetings in relatively isolated resorts creates a sense of camaraderie with fellow attendees from...depositor, discussed some of the many challenges of creating biological circuits, and I can only say this science...opportunity to share their cutting edge science in increasingly important field.  Interested in Addgene's ...

  9. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...single-strand DNA incorporation (ssDI). "Easi-CRISPR for creating knock-in and conditional knockout mouse models...Protoc. 13(1) (2018):195-215. PubMed PMID: 29266098 Creating knock-in mouse models with CRISPR has been challenging...efficiency. Easi-CRISPR uses a long ssDNA template to increase efficiency of insertion and gene replacement. ...

  10. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...we found that adding a TEV linker dramatically increased accessibility of the AviTag and HisTag, which ...fusion protein in bacteria. Our initial attempt to create a plasmid based on the biotin ligase BirA backbone... inserting BirA into a pMal plasmid (NEB) thus creating the pMal-T-Avi-His/BirA plasmid now available ...

  12. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...individual cells. Each cell is labeled with 3-4 tags to increase the diversity of the CellTag library. The fixed...detected over a 10-week period. Their expression decreased over time but they were not silenced.    Find ...retroviral overexpression cocktail yielded a three-fold increase of fully reprogrammed cells based on single-cell...

  13. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...University of Alberta and University of Oregon created mTFP1 by transforming E. coli with a gene library...tetramer-breaking mutations. The library was then screened through successive rounds of mutagenesis for the...filter. This combination results in a 2.6-fold increase in signal relative to mCerulean imaged with a ...

  14. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...R6K, and 15A. Not all origins of replication are created equal. Some will produce many plasmid copies and...because they will compete for the same machinery, creating an unstable and unpredictable environment. As ...plasmids if they contain large inserts or genes that create a toxic product. The E. coli strain Most E....

  15. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ... - not just for fiction! Image credit: Kenneth Rowe, under the creative commons license.    The introduction...is to find one or two roadblocks that sparked creativity, changed the outcome of the project, or illustrates...

  16. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...collection of antibodies available at launch were created by James Trimmer, PhD, of the University of California...commitment to supporting your research, Addgene is creating an array of educational materials to accompany...more useful to everyone over time. Through our incredible community, including your support, we have enabled...

  17. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...to select against CRISPR positive plants, but screening these can be challenging and requires specialized...Willoughby   Using RUBY as a selection marker The creators of RUBY highlight its use in crop plants under...as positive selection of transformed plants to decrease the routine usage of antibiotics which may contribute...

  18. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...primary antibody and endogenous IgGs in the tissue. Created with biorender.com. Strategies for avoiding...Some labs have found that you can significantly decrease background staining by mixing your primary and...you add your primary and secondary antibodies. Created with biorender.com. Proper tissue processing...

  19. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...Recombinant antibodies are plasmid-based and are created synthetically or derived from hybridoma antibody...antibodies against the new Fc region. In addition to increasing multiplexing capabilities this process is frequently...Although isotype conversion is routinely used to increase the utility of recombinant antibody tools, the...

  20. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...cassette allowing a researcher to perform blue-white screening after transformation - self joining of the vector...break down the steps needed for TOPO cloning: 1. Create Your PCR Product: Design standard primers (no need...unpaired 3’ ends in your PCR product. If you need to decrease error rate, please use one of these methods to...
Showing: 421 - 440 of 791 results