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Showing: 381 - 400 of 886 results
  1. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook...CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease, or both. Applications...
  2. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...Human Cell Differentiation Molecules group Addgene Data Hub Any (see notes) Any Data repository...repository Only accepts data for antibodies in Addgene’s repository  IBEX Knowledge-Base  Any IBEX...
  3. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...These vectors listed below should be released at Addgene in the next 6 months, pending quality control. ....1-WPRE-SV40  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...
  4. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful. Nothing more ...pMal-T-Avi-His/BirA plasmid now available through Addgene. We have successfully used this plasmid to biotinylate...cell signaling.     Additional Resources on the Addgene Blog Check out other Hot Plasmids Find Additional...Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular Plasmids in the Repository...
  5. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...For example, the many resources available on the Addgene website didn't come into being through the actions... Managerial Skill" Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists... Individual Development Plan Resources on the Addgene Website Are You a Molecular Biologist? Find Plasmids...
  6. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...received matches the expected sequence. Here at Addgene, we use NGS-based quality control to confirm the...necessary but also an easy process. Please vist Addgene's resource for plasmid verification to find additional...DNA gel.  More Plasmid Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols...
  7. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...resources on the Addgene blog: Introduction to Immunofluorescence View all of Addgene's antibody posts  ...Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ... found this blog helpful be sure to check out Addgene’s blog for other antibody topics.     References...
  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...contributing to the Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...
  9. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...resources on addgene.org Addgene's Antibody Guide Addgene's Ready-to-Use Antibody collection Addgene's Recombinant...R2 never dip below 0.95! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and...
  10. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...Resources at Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All ...and used in assays for over half a century; the Addgene blog has an excellent primer and tips from experts..., Pages 1348-1353. Additional Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn...
  11. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ... Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...that person before ever asking them for work. Addgene’s very own Joanne Kamens, a strong advocate for ...Scientists on docjobs.com Additional Resources on the Addgene Blog Check Out Career Topic Page Tips on Writing...
  12. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...Technical Support Calls Resources at Addgene Visit the Addgene Careers Page Check Out Our Career Videos...post. Klaus Wanisch is an Outreach Scientist at Addgene. These days he's doing a lot of science communication...cellular therapies.     Additional Resources on the Addgene Blog Read What Do I Do Now? Academic v. Non-Academic...this overall rewarding role. Learn about Other Addgenies and Their Careers in Our Career Videos What's ...
  13. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...synthetic? How Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org Check out...silence neurons in vivo (Slimko and Lester, 2003).  Addgene has a large selection of codon optimized genes,...embo-reports/kvd001 Additional resources on the Addgene blog Read more codon usage bias in Plasmids 101...out Addgene's Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection...
  14. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...ordering plasmids containing them from Addgene. If a plasmid at Addgene contains a gene that’s been codon ... for example). As many plasmids available from Addgene now have full sequence data, we recommend directly...PMCID: PMC2840511. Additional resources on the Addgene blog Plasmids 101: protein expression Plasmids...Plasmids 101 featured topic page Resources on Addgene.org Molecular biology reference pages Genetic code...
  15. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...released: Addgene's Plasmids 101 eBook And now for some exciting news... You can now download Addgene's new ...depending on the placement of the reporter gene). Addgene's collection offers a variety of plasmids to express...PubMed.   Read more Plasmids 101 posts or browse Addgene's Plasmid Guide for more molecular biology and cloning...
  16. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...antibodies Resources on Addgene.org Find written and video protocols Visit Addgene's antibodies collection...plasmid encoding your protein of interest from Addgene, transfected it into your target cells, now what...may even find plasmids encoding antibodies from Addgene that you can make on your own.  To narrow your ... found this blog helpful be sure to check out Addgene’s blog for overviews of other antibody topics. ...10.4103/1947-2714.100998 Additional resources on the Addgene blog Find other tips and tricks for molecular ...
  17. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene. The Podgorski ...References and Resources Additional Resources on the Addgene Blog Learn about other Fluorescent Biosensors ...Fluorescent Protein Featured Topic Page Resources at Addgene.org Find Fluorescent Protein Biosensors Browse All...
  18. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody...www.ncbi.nlm.nih.gov/books/NBK100203/ Additional resource on the Addgene blog Learn more about research applications for...
  19. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...among the first batch of antibodies we produced at Addgene and, as you might suspect, it has been one of our...always best to have options.      Fig. 2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662... [N86/8R] page for more details. Image credit: Addgene.   Find anti-GFP [N86/8R] here!   Andrews NP...
Showing: 381 - 400 of 886 results