We narrowed to 258 results for: nif
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TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....development happening at Addgene under the hood. A significant portion of our development may not be visible... been updated in several years) or they make significant changes that make it incompatible with our software...software from Python 2.7 to Python 3.5. There are significant changes to the language and it took us a total...
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New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....enzyme can lead to off targets and result in significant, negative effects in RNA targeting experiments...non-specific activity. Its major advantage is the significantly reduced amount of off-target toxicity compared...using Prime editing alongside some creative (and significant!) protein engineering. They combined Prime editing... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m....you! Many thanks to our Guest Blogger, Jennifer Rivers! Jennifer Rivers ScM, MLS(ASCP)CM is a research ...This post was contributed by guest blogger, Jennifer Rivers, a research microbiologist specializing in... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m.... the cytoplasm? Or more troubling, what if a significant fraction of the fusion protein molecules fail...resident proteins within these compartments undergo significant post-translational modifications including glycosylation...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....vastly expanded axolotl research. One of the most significant advances is the sequencing of the entire axolotl...research providing insight into some of the most significant and fundamental biological processes. Many of...widespread utilization, Arabidopsis has made significant contributions to understanding plant and crop... -
CRISPR 101: Off-Target Effects
TypeBlog PostPublishedFeb. 8, 2024, 2:15 p.m.... to keep in mind, but likely will not have a significant impact on your data. You are trying to generate...selectively sequenced to determine if there are significant editing events, serving as a proxy for total ... to effectively control for off-targets and significantly decrease the probability of artifacts. Off-target... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog PostPublishedNov. 6, 2015, 3:30 p.m....that their knowledge and skills had improved significantly when compared to their pre-program self-assesment...improvements, not necessarily quantifiable, but always significant. Doubts, fears, insecurities… all shrink when...generations of professionals has made possible a significant qualitative and quantitative expansion in the... -
Addgene is Stopping Use of X (formerly Twitter)
TypeBlog PostPublishedJan. 11, 2024, 2:15 p.m.... suggestions in the comments. X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog PostPublishedDec. 5, 2013, 3:27 p.m.... have your first cold of the season like I do, sniffle) ourselves back into our regular post-holiday routines...Researchers in Taiwan showed that a laughter program significantly decreased immunological markers of stress in... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog PostPublishedJan. 17, 2020, 3 p.m....MCDB 🎄🎅🎁🐸🐸🐸🐸 pic.twitter.com/2MPLawgNQl — Jennifer Landino (@jenelandino) December 3, 2019 Hope... Jennifer Tsang... -
DIY DNA Ladders from Penn State University
TypeBlog PostPublishedJuly 14, 2017, 2:30 p.m....worth of ladder. The Penn State ladders are significantly cheaper. When using a homemade plasmid prep ...increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are you interested... -
What's New in CRISPR - Winter 2018
TypeBlog PostPublishedDec. 18, 2018, 1:17 p.m....genome editing toolkit New Cas protein identified Jennifer Doudna’s lab recently identified Cas14, a family... Jennifer Tsang... -
Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m....there's a pretty good reason for that. Lately, significant advances in the understanding and application... work. Last month, the Lawrence Berkeley Lab's Jennifer Doudna and Eva Nogales presented the first detailed... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m....a bioinformatics and a screening approach, the Jennifer Doudna lab identified three different Cas12a inhibitors... Jennifer Tsang... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m....that lab and interacting with the lab members.”- Jennifer Tsang, Science Communications and Marketing Coordinator... Jennifer Tsang... -
What's New in CRISPR - March 2020
TypeBlog PostPublishedMarch 10, 2020, 1:15 p.m....fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of multiplexed gRNAs in... Jennifer Tsang... -
What's New in CRISPR - September 2019
TypeBlog PostPublishedSept. 10, 2019, 12:56 p.m....active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered a new Cas protein, CasX... Jennifer Tsang... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....discovers of CRISPR/Cas9 genome engineering, Dr. Jennifer Doudna and Dr. Emmanuelle Charpentier. I was joined...especially excited to attend this event because Jennifer did her graduate work one floor above mine in ... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....excitation maximum 399 nm) because it does not significantly excite at 400 nm while EGFP does. Find mWasabi... Jennifer Tsang... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....IgGs and your primary antibody, resulting in significant background staining (Figure 1A). Not all tissues...Figure 2B) Some labs have found that you can significantly decrease background staining by mixing your ...