... using a chemical transfection reagent (e.g. PEI-MAX). Simply cotransfect a mixture of 1248 ng of CMV-...

...Suppmann, the Recombinant Protein Production group at Max-Planck Institute of Biochemistry has developed a ...Multisite Gateway compatible destination vector for a maximum of 24 elements assembled into a final transgenesis...

...interaction strengths. Erich Wanker’s group at the Max-Delbrueck Center for Molecular Medicine has recently...independent gRNAs, each chosen strategically to maximize the likelihood of creating a functional knock-...

...indel frequency. Koblan et al., 2018 BE4max, ArcBe4max, ABEmax   Improved nuclear localization and expression...localization signals and codon usage BE4 to create BE4max and AncBE4max with a 4.2-6-fold improvement in editing...adenine base editing When the Liu lab created BE4max and AncBE4max mentioned above, they also made an adenine...., 2020 SpRY Cas9, available fused to ABEmax(7.10) or to BE4max   Evolved SpCas9 with NRN or NYN PAM site... more flexible sequence preferences, evoAPOBEC1-BE4max, which is significantly more effective at editing...localization and expression. This base editor was named ABEmax. In 2020, two papers were published describing ...programmable adenine and cytosine editor) by fusing miniABEmax-V82G and Target-AID to Cas9 (Grünewald et al....

... continued to improve the maximum efficiency of prime editing. While maximum efficiency isn’t everything... PE2-5 approaches, sometimes referred to as PE2max, PE3max, etc. PE6: Specialized prime editing variants...end of PEmax to produce PE7. PE7 significantly improves editing efficiencies compared to PEmax with normal... originally introduced, rely on the PE2 enzyme. PEmax: Optimizing prime editor sequence to improve expression...be combined with any of the PE2-PE5 strategies. PEmax contains a reverse transcriptase with a sequence... although both enzymes still approach or exceed PEmax editing efficiencies for short, simple edits. PE6c... Innovation Purpose PE2 PE3 PE4 PE5 PEmax PE6 PE7 Pentamutant reverse transcriptase...

...spectrum shows two maxima, one local maximum at just under 500 nm and a global maximum at around 560 nm,... global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If...and emission spectra. Both spectra possess clear maxima where excitation and emission are the most efficient...fluorophores. Each fluorophore has a defined emission maximum. Note, you can also use the tool to display excitation...wavelength of 488 nm (Table 1), which is near a local maximum of PE's excitation spectrum (dotted line, Figure...excites at 561 nm, which is close to the global maximum of PE's excitation spectrum where the excitation...

...mutations). Its excitation maximum is reported as 603 nm, while the emission maximum is 675 nm. mCarmine is...boundaries of FP technology by shifting the emission maxima toward the red end of the spectrum (above 600 nm...

...molecules have a specific wavelength that they maximally absorb. The spectrophotometer measures these absorbances...contaminants. Nucleic acids (DNA and RNA) absorb maximally at 260 nm. Proteins on the other hand absorb best...nm and organic compounds and chaotropic salts maximally absorb at 230 nm. The A260/A280 ratio is used ...

...uniquely positioned to help research institutions maximize their increasingly limited resources: Preserving...resources become even more valuable as labs look to maximize productivity and efficiency with fewer resources...

...we are processing 159 more requests." Pool and maximize resources nationally and internationally COVID...McGill University. “There is a need to pool and maximize resources nationally and internationally such ...

...deamination. They generated these editors using the BE4max architecture. The new editors reported in this ...average on-target DNA-editing levels relative to BE4max.  Read the paper in Nature Biotechnology Find ...

...Kcleave (min-1). SpCas9 has maximum activity from 35-45 °C, while GeoCas9 has maximum activity from 50-70 °C...

...amino acid sequence of the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and...disease.  Read the paper in Cell Find PE4, PE5, and PEmax plasmids Precise genomic deletions In PRIME-del...

...advancements in gRNA design necessary to achieve maximal on-target and minimal off-target activities have...Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

...through several rounds of stripping and reprobing, maximize your signal by starting with the least-abundant...Immediately after transfer, dry the membrane to maximize protein retention. Following this step, most PVDF...

...infectious viral particles must be high enough to maximize possible outcomes. Typically, AAVs have to be ...108 IU/ml (infectious units per ml)  in order to maximize possible effects. Injection Parameters: The injection...

... JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna JA (2017) A Broad-Spectrum Inhibitor...Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR (2016) Naturally Occurring Off-...

...1: Left: recombined prime editing tools PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA...

...growth time, temperature, and shaking speed to maximize cell density and plasmid yield as these factors...need to be grown for 20 hours or more to achieve maximum plasmid yield. The optimal growth time should be...

...dilution series of the standard and the minimum and maximum detection limits of the instrument being used to...specificity, especially as it approaches the minimum and maximum thresholds. If the measurements for the unknown...