Light Sheet Fluorescence Microscopy
Type
Blog Post
Published
July 16, 2015, 2:30 p.m.
...photo-bleaching or photo-damage than in conventional laser scanning microscopy . Since image acquisition is performed...milliseconds to acquire one image, thereby significantly reducing the time it takes to image through a large z-...the acquired image (5). A way to get around this problem is to illuminate and acquire images of a sample...the 3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal... axial resolution and reduce phototoxicity for imaging live specimens, they combined light sheet microscopy...sequential multiple-view reconstruction (5). In order to image large tissue such as cleared rodent brain, the Ultramicroscope...point-by-point scanning along the entire specimen makes imaging too slow and unwieldy for large specimens that ...