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  1. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...repression), and Fok1-dCas9 (dimeric nuclease). Gateway assembly method Frew Lab Multiple Lentiviral Expression...should be compatible with type IIS enzyme BfuAI. Gateway cloning is then used to combine the multiple gRNAs...

  2. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...Resources on the Addgene Blog Learn More about Gateway Cloning Gibson Assembly and Other Long-Homology...

  3. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...example is the ccdB gene system commonly used in Gateway® Cloning. There are also methods to select for ...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...The collection is compatible with both standard Gateway cloning and the FNLCR Combinatorial Cloning Platform...mammalian systems. The DULIP system is comprised of Gateway-compatible plasmids in which “bait” proteins are...

  7. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein expression...

  9. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...may require strains such as ccdB survival (for Gateway plasmids).  We also like to store plasmids that...

  12. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest...

  16. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...page. The data produced is available on the F1000 gateway and Zenodo community.  Knockdown approach For...
Showing: 21 - 37 of 37 results