...end up stressed and rushing, no matter how hard I tried to build in extra time before the experiment. What...any experiment. That, more than anything else I tried, made a huge difference in my ability to focus, ...used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu/robertsonlab...

...modular code. Use tried-and-true tools We use Python, Django, Apache, and MariaDB. Using tried-and-true tools...tracked. Terminology and language are tricky Names for things will trip everyone up at some point, decide ...messiness of biology. These examples are intentionally trivial, but they're here to help tell this story. In this...plasmid and now it’s time for our lab technicians to retrieve the sample and get it ready for shipping. In the...contents might leave you sad and confused when a tricky procedure failed and one of your techs started ...

...biology. While not everything is open-access, I have tried to prioritize open-access resources whenever possible...

...plasmids for transfer to recipient cells during a tri-parental mating scheme. Mobile-CRISPRi integrates...transferred to a recipient bacteria strain of interest by tri-parental mating. Donor bacteria contain a chromosomal...presence of trimethoprim, cultures treated with a folA gRNA were more sensitive to trimethoprim when compared... which encodes the target for the antibiotic trimethoprim. When Enterobacter aerogenes, Klebsiella pneumoniae... the folA gene product not been a target for trimethoprim, there would be no changes in sensitivity. Pooled...

...and lead to you being remembered as a person who tries to find solutions that benefit everyone. Even if...negotiation, I will view the “winner” positively if they tried to understand my position and to find a creative...

...have dedicated science teachers. I tried contacting our school district’s science curriculum coordinator ...the technology in an unethical manner, but I also tried not to be an alarmist.  The overarching guideline...and science communicator, and I strive to be a volunteer and contribute to my community. This past year...all of these roles by reaching out to my school district not just as a dad, but as a scientist. When our...it. PTAs have more flexibility than the school district itself in terms of what they do with their discretionary...like this without them! If you live in a school district that has a lot of parents in STEM careers, chances...chances are they’re wondering how they could contribute to the school, too. Though you can recruit parents...

...month ago. Why haven’t we received our order? I tried searching my PO in the track order portal and nothing... Ordering genes on plasmids online is a little trickier -- but at Addgene, we try to make the process ...does not own the material in our repository. We distribute it on behalf of depositing institutions. Addgene...

... Paolo Colombi Should I store my plasmid in TE, Tris, or water? And what about temperature? Find the ...

...for efficient recombination? A3: The most we've tried to insert so far has been 1kb. We used homology ...template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) (see Figure...

...the grant or your PI may edit everything you’ve tried to draft, but you’ll still be practicing and getting...

...is really important. For my Postdoc position, I tried to read at least 50 papers on the topic before I...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well...

...molecules are sensitive to blue light. Many have tried to make green molecules, but then alongside blue...Optogenetics is a toolset that lets you turn the electrical activity of neurons on or off using pulses of...neuron or set of neurons, you can see how it contributes to behavior or pathology, and by disabling neurons...deafness, or other conditions. Addgene: You've contributed various plasmids to Addgene over the years, many...popular. Why has it been so important to you to distribute these tools as freely as possible? Boyden: I ...for different ions. Right now, we can modulate electricity. If you could selectively let in calcium or some...brain. Addgene: Do you have any special tips or tricks for someone looking to try these optogenetic tools...

... Maguire and colleagues, other researchers have tried to modify it to improve yields. For example, transfecting...for isolating exo-AAVs rely on differential centrifugation steps to separate cell culture supernatant ...

...started learning proper cell culture technique, I tried to find an app that could help me with calculations...information in the app is restricted to the NEB inventory only, the restriction enzyme sequence resource...This post was contributed by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article...comes in handy when I use protocols that require restriction enzymes or PCR. The app contains a double digest...digest tool that is only helpful for NEB restriction enzymes since the instructions use NEB buffers. Nonetheless...name to discover all the information about that restriction enzyme or share it via email with a colleague...colleague. The app is also great if you’re adding a restriction site to a primer, you only need to skim the alphabet...

...tools are abundant! In this post, we will review tried-and-true protein expression control tools as well...

...marker at non-physiological levels. Scientists have tried varied approaches to combat these issues. One method...maintained when grown on media containing the missing nutrient. Scientists can exploit these host mutations by...auxotrophy. When grown on media NOT containing the nutrient, the host cells will die unless they have incorporated...  Marcy Patrick...

...these elusive teachers, supporters and advisors? I tried to start a more formal mentoring program at my company...They all found the support of the group to be intrinsic to their success. Their stories made it clear ...Goal setting is easier with more perspectives contributing to the process Senior people (of which there...

...most widely known of these products is TRIzol® (also called TRI Reagent®, RNAzol®, QIAzol®  and sold by...homogenate and shaken. After a quick centrifugation (not ultracentrifugation!), the layers of phenol and chloroform...After homogenization in TRIzol®, insoluble material is removed via centrifugation and the supernatant is... loaded onto cesium-chloride gradients and ultracentrifuged for up to 20 hours to separate the intact ...have, access to one or more large, expensive ultracentrifuge.  Figure 1: An outline of the different...out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski and Sacchi showed... by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol solution that combines...

...confused, bored, or particularly excited about? If you tried tweaking your pitch throughout the conference, what...

...writing three years ago on a field trip in Indonesia. Since then, I have tried many things: photography, making...This post was contributed by guest blogger Sarah Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory...hosted by Nature), and Addgene are open to guest contributions. If you decide to start your own blog, check...