... some you’ve likely never heard of. Each entry displays the following information for a Cas enzyme: ...properties Links to relevant resources The homepage displays a graphic describing CasIDs and relevant properties...feature. Activity features: CasID CasPEDIA will display both the cis and trans activity of each entry. ...protospacer-flanking sequence (PFS) in some cases. The resource displays these requirements in regard to their sequence... covered!     Fig. 1 – CasPEDIA Cas ID display information for a given Cas (SpyCas9a) from CasPEDIA...

...excited to announce that our plasmid and sequence displays are now powered by GSL Biotech's SnapGene Server...feature library, our updated plasmid and sequence displays are now much easier to interpret and analyze at...CRISPR gRNA cloning. With this more functional display, you can start thinking about your next cloning...experiment early on. Beyond these simple but powerful display improvements, when you click on any plasmid map...enhancements that will further improve the map displays and functions. ...

...scientists can mutate the transcription start site, splice sites, exons, and/or promoters to knock out lncRNA... and Bester 2021; Zibitt et al. 2021). Mutating splice sites is particularly effective as it can result...Transcription start site (TSS) deletion. (B) Mutations of splice sites. (C) Removal of an exon or a large genomic...noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol. Published online November...expression by lncRNA promoters, transcription, and splicing. Nature. 2016;539(7629):452. doi:10.1038/nature20149...

.... This style displays a poster vertically rather than horizontally. Instead of displaying your information...employing a large central box, often used for displaying all of one’s figures, or at least the majority...inserted. The advantage of this style is that it will display your data most prominently, making it the star ...information as columns, you may choose to display it as rows. It can be more catchy because it is generally...

...performed via phage display, yeast display, ribosome display, or mammalian display (Tsuruta et al., 2017...10.1038/srep31730 Tsuruta LR, dos ML, Moro AM (2018) Display Technologies for the Selection of Monoclonal Antibodies...

...range of PAMs like NG, GAA, and GAT, but also displaying increased editing specificity. We’re excited ...recognizing NG, NNG, GAT, and CAA PAMs, while xCas9 3.6 displayed the second best PAM range. Characterizing xCas9... SpCas9 in mammalian cells, finding xCas9 3.7 displayed about the same editing rate as SpCas9 at an NGG...SpCas9. For some non-promiscuous gRNAs, xCas9 3.7 displayed more than a 100 fold decrease in off-target editing...ratio. Importantly, the observed off-target loci displayed a broad range of PAM sequences, indicating they...

...detecting protein-protein interactions that uses split fluorescent proteins tethered to potential protein...visualize PPIs. This fluorescent protein (FP) is split into two fragments that individually lack fluorescence...fluorescence microscopy. Figure 1: How BiFC works. A split FP (e.g., yellow fluorescent protein, YFP) is reunited...information about the structures of the tested PIPs. Many split FPs have been validated for BiFC (Kodama and Hu,... Hu, 2012). Such controls are necessary because split FPs can associate, albeit at relatively low frequencies...

...anchoring domains The original iGluSnFR in the pMinDisplay backbone is fused to a specific peptide segment...TMD) that anchors the sensor to the membrane and displays it on the extracellular side. In addition to the... 4 Sequence schematics for PDGFR, GPI, and SGZ display constructs. Image adapted from Aggarwal et al… ...transmembrane domain in the mammalian expression pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol...

...array targeting the MAPT exon 10 splice acceptor and two putative splice enhancers. After AAV-mediated delivery...previously characterized Cas13 enzymes, CasRx also displays very high on-target efficiency; where shRNA treatment...produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9, for which efficiency varies...

...following a G more efficiently than BE3, but APOBEC3G displayed less predictable sequence preferences.  The Liu...editors (ABEs). ABE7.10 is the most active editor, displaying an average editing efficiency of 53% with an ...of target positions 4-7. ABEs 6.3, 7.8, and 7.9 display slightly wider editing windows of position 4-9,...produce a mixed population of edits, ABEs do not display significant A to non-G conversion at target loci...Highlights Komor et al., 2016 BE1, BE2, BE3   BE3 displays highest editing efficiency but higher indel formation...

...from the Mark Dawson lab Re-transformation of the SPLINTR Libraries Protocol.   Selecting your strain...perform several individual transformations, such as splitting 400 ng of DNA in 100 μL of cells over four individual...Electroporation Protocol (DOCX, 15.8 KB) Barcode SPLINTR Pooled Libraries 200,000–1,200,000 60 bp ... 200 ng Liquid Re-transformation of the SPLINTR Libraries (PDF, 401 KB) Screening Zhang Lab...

...can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be split into two halves, which are...proximity, they will form the full FP and fluoresce. Split FPs can be used to determine the proximity of two...two proteins fused to the halves of the split FP. This technique is also is also called Bimolecular Fluorescence...

...fusion proteins for imaging, tracking, modulating splicing, and regulating expression of specifically targeted...described in bacterial cells, LwaCas13a does not display nonspecific RNA cleavage activity in eukaryotic... LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant...to see this group of enzymes continue to make a splash in the CRISPR field. We feel that the culture of...

... of interest do not lie beyond the dimensions displayed in the plot. (B) A more restrictive gate results... second measurement to choose for graphically displaying your events. You are free to choose any size-... the positioning and shape of the populations displayed. As the separation pattern largely stays the same... cells but not dendritic cells. Using a graph displaying both stains, I can quickly separate T killer ...

...antibodies (rAbs) either produced de novo using yeast display technology or produced from hybridoma antibodies...The 11 IPI synthetic rAbs, developed using yeast display technology, are directed to integrins that bind...fragments (Fabs) that bound an amino acid region displayed by a subset of the RGD-binding integrins containing...Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits...

...proteins (PA-FPs) are fluorescent proteins that display unique changes in their spectral properties upon...population that is more neutral, causing PA-GFP to display almost no fluorescence when illuminated with 488nm...fact, many of the early reversible PA-FPs also display this “negative” photoswitching. In 2008, scientists...than mEos2 (mEos4b is completely monomeric) and display bright and photostable green and red states. Beyond...

... a wide audience. The dashboard that I made to display the data in the CSV file is open for anyone to ...Addgene Requests Dashboard   The dashboard will display the following items (Fig. 1): A row with four... the CSV is shown (figure 2) and the dashboard displays the data in a standardized way. Since Addgene ...

...(AAV). The components of these vectors are also split across several plasmids (often three), for safety...virus must assemble and have appropriate factors displayed on the outside of the particle to interact with...components needed for viral packaging, assembly, and display. Pol, the last component, contains the reverse ...

...long-time Addgene depositor Alice Ting used yeast display-based directed evolution to create APEX2. They ...landscape of the SH3 proteins using peptide-phage display and deep sequencing, revealing that they bind to...molecule binding screens, peptide arrays, and phage display selections. In addition to these constructs, the...

...release. How is this light-mediated? The researchers split the proteolytically active light chain of BoNT/B...high affinity binding to SspB, brings together the split botulinum neurotoxin halves to enable VAMP2 cleavage...can alter motor responses in C. elegans. Find the split BoTN/B plasmids at Addgene. Liu Q et al., Neuron...