Visualizing Genomic Loci with CRISPR-Sirius
Type
Blog Post
Published
April 18, 2019, 12:53 p.m.
...guide RNA sequence.
A year later, they developed CRISPRainbow (Ma et al., 2016), a set of gRNA scaffolds that...three hairpins produces a white color. While CRISPRainbow allows for simultaneous visualization of sites... are relatively unstable.
Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences inserted... of the MS2 and PP7 RNA hairpin sequences in CRISPRainbow, they knew they could be good candidates for...performed, they compared them head-to-head against CRISPRainbow. Both tools were targeted to the FBN3 intronic...noise ratio can be increased from 2-fold (by CRISPRainbow) to 3-fold with CRISPR-Sirius.
Triple color ...used in live cells. Compared to its predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable...