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  3. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... actually infect cells. These assays typically involve infecting a target cell line with your virus and...

  9. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...probably best to avoid AID tags. If your project also involves imaging at some stage, then choosing a HaloTag...

  10. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...specific cell types is where viral vectors become invaluable. Viral vectors are mechanisms by which genetic...

  13. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...

  14. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...with repeat elements, such as viral plasmids with inverted terminal repeats. We use NEB Stable to transform...

  15. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...? Depositing lab: The name of the Principal Investigator (PI) in whose lab the construct was made. The...

  18. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...blastn or blastp, due to the additional searches involved in translating the nucleotide sequence into all...
Showing: 361 - 380 of 442 results