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  2. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...the FP (fused to the protein of interest), thus tracking the localization of that biomolecule inside living...

  6. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... knob domain, and hexon), as well as other intracellularly-exposed proteins and downstream cellular events...

  7. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...500 bp to 1 kb range, however, shorter homology tracts have recently been used with some success (Yu et...

  8. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...but they can also be expressed in bacteria and extracted with common protein purification methods. Read...

  11. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...place.  When the PCR is complete, a droplet reader extracts the droplets from the plate and measures the fluorescence...

  12. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...the stress of selection you can clip a leaf and extract genomic DNA for genotyping. This won’t hurt your...

  13. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ... quickly – modifying the length a polyhistidine tract does not greatly alter the size of the tag.   Table...

  15. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...screening with CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org Addgene's most...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...data for our DNA-based reagents. Not only do we extract as much information as possible from depositing...

  17. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...strengths.    . Steps of an IP: 1) Protein extracts from cells or tissues contain a complex mix of...
Showing: 361 - 380 of 425 results