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  1. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...gene of interest, but after an internal ribosome entry site (IRES). This is oftentimes used in conjuction...Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct...

  2. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...that can impede collaborations, especially with industry. Today we also have the appropriate tools to verify...the European distributor for ATCC. He studied biochemistry at Warwick University, gaining extensive cell...

  3. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...brightness of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore ...has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult to address especially...

  4. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...popular bacterial organism in microbiology and biochemistry. Certain strains have been specifically developed...studies involving axolotls focus on embryonics and biochemistry. Though limited at first, advances in axolotl...

  5. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...applications like fluorescent labeling via click chemistry.    Figure 4: SuperPLD hydrolyzes membrane PC... a membrane editor in mammalian cells. Nature Chemistry, 15(7), 7. https://doi.org/10.1038/s41557-023-...

  6. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...and U6) and measuring Corn fluorescence by flow cytometry, the Jaffrey group was able to quantify the effect...fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908. PubMed Central...

  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...receptors, co-receptors, and other mechanisms to gain entry into the cell (Dudek et al., 2018; Korneyenkov &...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. Journal of Virology...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ..., label chromatin, and study kinetics. Using chemistry to photocage CRISPRs The aforementioned techniques...-based Photoactivatable Transcription System. Chemistry & Biology 22:169–174 . https://doi.org/10.1016...

  13. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...reagents available to scientists in both academia and industry. This year, we also launched the cloning grade...

  14. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...Individual Plasmid Formats Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer...

  15. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...for another part of my job, I travel around the country and set up meetings with PIs to discuss the benefits...

  16. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ... work experience I had was in the food service industry, which followed a very different schedule. Fortunately...
Showing: 321 - 340 of 441 results