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We narrowed to 675 results for: eng

Showing: 321 - 340 of 675 results

  1. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...technology has made it easier than ever both to engineer specific DNA edits and to perform functional screens...really due to the intended edit rather than some passenger variant that came along with the single cell clone...development of SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers additional PAM options...

  2. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...introduces a ssDNA nick, a dsDNA break, or is engineered not to cleave DNA and instead activates/represses...acknowledged as a potential limitation in a study. Strength in numbers If you assess your off-target frequency...Off-target effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389...

  3. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...12259: pMD2.G (depicted on the left) is ~5.8 kb in length. The plasmid can exist on as a dimer, depicted ...tandem, resulting in a plasmid that is twice the length of the monomer (~11.6 kb). Image generated by SnapGene...and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43. doi: 10.1002/bit.22347...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...Preps at Addgene 2) Comparison of genetically engineered receptors (i.e. chemogenetics vs. optogenetics...transduction efficiency, and safety profiles should strengthen its appeal for the scientific and medical communities...techniques: exploiting the offspring of genetic engineering." Journal of neuroscience methods 235 (2014):...

  5. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...that code for surface proteins. One of the first challenges with a custom screen approach is to identify ...PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries..., Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....

  6. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...then flow cytometry can help you overcome these challenges and make these types of experiments easier and...fluorescent signal that is captured by detectors. The strength of the emitted photons varies from cell to cell...fluorophores Fluorescent antibodies get around the challenges of fluorescent protein fusions by binding to ...

  7. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...enabling a broad spectrum of donor fluorescence wavelengths for FRET experiments. However, remember that,...amino acid at a stop codon (often amber i.e. UAG) engineered into a desired site. During translation, the ...molecules to each Cy dye, as well as by extending the length of the histidine tag from 6 to 10 residues. While...

  9. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...the second day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency. ...CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth day of CRISPR, Addgene...

  10. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...temperature reduces background noise but increases the length of your assay. The most common options are 4 °C...background noise as well, and multiplexing is challenging and limited. Direct westerns typically rely on...waste. However, they also add time to an already lengthy protocol, require the use of hazardous chemicals...

  11. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    Published
    Sept. 5, 2018, 12:01 p.m.
    ...and Jillian Parker! Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...

  12. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...-defined contours. (Many of them are software engineers, surprisingly, who have deep technical knowledge...editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also writes Codon, a blog...

  13. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ...twitter @aigilson.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  14. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...Bacterial Expression Systems. Check out our Genome Engineering Overview and our CRISPR guide to find out how...how CRISPR is being used for bacterial genome engineering. To make it easier for you to find plasmids ...

  15. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ...sciencewithtom.com.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  17. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...Yeast Prototrophy Kit Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  18. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...Vincent's tweets.      Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  19. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...Vincent's tweets.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  20. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...You can try a few different lysing buffers and strengths. If that’s not working, consider using a subcellular...incubation (10–20 minutes) at 70 °C, or a truly lengthy incubation (30–60 minutes) at 37 °C, if you suspect... Different lysing buffer/adjust lysing buffer strength Sonication based lysing protocol Subcellular...
Showing: 321 - 340 of 675 results