...label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable development..., then the other, a dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels...their initial tests with thick slice (70 μm) immunohistochemistry (IHC) (Figure 5). PSD-95 is a scaffolding...

... unconjugated and recommended for use in flow cytometry. Based on the established applications of the ...will also work in immunoprecipitation and immunohistochemistry. Table 1: IPI antibodies  Antibody ...Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...

... chemically pre-treating the cells to allow for entry of the antibodies. Here, a two-step process is employed.... The main applications of dye stains in flow cytometry are cell proliferation and cell viability. Unlike...101: Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

...more stable yet brighter furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp...successfully infected/expressing cells by flow cytometry. The isolated cells were injected into mice and...enable sensitive in vivo optical imaging and flow cytometry for monitoring tumorigenesis. Cancer Res. 75, ...

... contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University of Cambridge...standardized validation techniques for academia, industry, and especially the clinic. Popular validation...contributions to this work. Søren Hough is a Biochemistry PhD candidate in the laboratory of Prof Steve...

...the top right of each data entry page. Once you have completed the data entry process for all of the plasmids...Request Deposit Kit.” button to complete the data entry. Clicking this button will allow you to choose which...

...Although only wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors containing...expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the U6 promoter and the gRNA ...

... if an export license is not required for your country after checkout, and the processing fee will be ...licenses is available through the US Bureau of Industry and Security. If you have any questions about ...

...serotype, promoter, or tool type. Drilling into any entry will reveal additional data including the subject...injection before performing the experiment. Every entry has an accompanying image so that you can see for...

... The Tarling-Vallim Lab’s first place-winning entry doubled as their lab’s decoration competition, and...snowflakes and viruses both exhibit similar forms of symmetry. 🎶Walking in a Winter Wonderlab🎶 We decided...

...date, material, requesting PI & organization, and country. This information can be of personal interest to...horizontal bar chart) Number of requests per country (as dot plot) Number of requests per year (as...

... the overall plasmid selection will be open to industry scientists. This collection is highly diverse ...controlled through next generation sequencing and spectrophotometry to test purity (260/280 nm and 260/230 nm ...

... data, serve society and patients, and respect industry concerns.” 5)      In the aftermath of the Supreme...factor, study topic, and the corresponding author's country of origin, were taken into account. 7)     Science...

...There are also different regulations from country to country when it comes to administering phage therapy...

...-Niang (above, left) is an Full Professor in Biochemistry with a background in virology who has worked...Jun 15;134:1-17. PubMed PMID: 27984002. 2. Bilan, Dmitry S. et al. “HyPer-3: A Genetically Encoded H2O2 ...

...was unsure whether or not I wanted to major in biochemistry. There, I had exchanged contact with Amy C.,...internship at Addgene. They plan on majoring in biochemistry in college and play the drum set as their personal...

...tags are frequently used for western blot, flow cytometry, and immunofluorescence . However, protein purification...tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence where an antibody can’t...

...can directly interfere with a seamless jump into industry.” In his article On Leaving Academe, Terran Lane...budget constraints Academic science is much like industry but the “product” is publications—the pressure...

...discernible paths. The first leads into the world of industry. Along this road, science communication graduates...ranged from wildlife conservation to abortion law to poetry. A research discipline in its own right, science...

...neuroscience and is available to both academic and industry scientists.  The collection of antibodies available...2019), validated in both western blot and immunohistochemistry. Additionally, many of the hybridomas from...