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We narrowed to 676 results for: eng

Showing: 281 - 300 of 676 results

  1. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...this virus. While various Cas9 mutants have been engineered to perform unique editing tasks, classic Cas9...LentiCas9-blast is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al...makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity also causes growth rate variability...

  2. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...computation work comes with new sets of challenges. One challenge with computational analyses is to keep... are doing what you are doing. Another common challenge is to keep track of what works, and what does ...

  3. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...advantage to using the CRISPR/Cas system for genome engineering is the fact that Cas9 can be easily programmed...smegmatis (Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair...UNC Chapel Hill.   References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM (2009) Limiting the Persistence...

  5. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ... evaluate degradation of ENL. Cell lines were engineered to express ENL-FKBP12F36V and endogenous ENL ...evaluate degradation of MELK. Cell lines were engineered to express FKBP12F36V-MELK and endogenous MELK... evaluate degradation of YY1. Cell lines were engineered to express YY1-FKBP12F36V using CRISPR/Cas9-mediated...

  6. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...injected with an immunogen. This might be the full-length protein that you want to generate antibodies against...Otherwise, you might experience variability in the strength of staining and background noise. If you do use...control across experiments to be aware of signal strength. In general, polyclonal antibodies produce a higher...

  7. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...excitation wavelength. A fluorophore is less likely to bleach when it is excited off the peak wavelength, since... excitation powers are often used to reduce the length of the experiments. As the photobleaching rate ...

  8. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. ...Consulting works remotely, will travel for speaking engagements and has many areas of specialty from leadership...but the website looks interesting and it is in English and Spanish. She is based in Spain. The bottom ...

  9. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability ...tool to the CRISPR tool box.  Nanoblades are engineered murine leukemia virus (MLV)-like particles loaded...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades...

  10. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...high-complexity reactions? The researchers over at New England Biolabs® thought otherwise! They suspected a data-driven... Golden Gate Assemblies. Image courtesy of New England Biolabs.   DAD Tools The three DAD tools used...Temperature Incubation time/cycling Overhang length T4 DNA ligase 25 °C 1 hour 3-base or ...

  11. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...literature! PubMed PubMed is the classic search engine for finding papers. It’s been around since 1996...Zuckerberg Initiative. Meta is not a traditional search engine. Instead, you create your own research feed by ...

  12. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...its ground state by emission of a photon with wavelength specific to the acceptor In order for FRET to...quantify mechanical tension (a molecular “spring”) (Meng et al., 2008), and monitor neuronal activity (voltage...Nature 388.6645 (1997): 882. PubMed PMID: 9278050. 3. Meng, Fanjie, et al. "A fluorescence energy transfer‐...

  13. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Note: Cpf1 is also called Cas12a. In 2015, Feng Zhang’s lab characterized two Cpf1 nucleases, distant...editing. LbCpf1 and AsCpf1 are 3.7 kb and 3.9 kb in length, respectively, larger than SaCas9 but smaller than...deletions at the 3’ end of the target sequence. Cpf1’s strength may lie in its low off-target editing rates, determined...

  14. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...fiber optics needed for optogenetics. First, the length of the fiber optic for the implant depends on the...started, you will need a laser of the appropriate wavelength to stimulate your opsin. Each opsin has an excitation...fiber optic patch cable must be the appropriate length for your specific behavioral apparatus or test ...

  15. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...scientists work to invent novel techniques or to engineer improved tools for familiar applications. But ...variants of MagLOV will be possible with other engineering approaches and that magnetosensitive tools can...biophysics, as well as inventors and biomedical engineers, join efforts to explore the mechanisms and develop...

  16. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ... to lab-adapted bacteria because it has been challenging to introduce CRISPRi machinery into diverse bacteria...antibiotics are often essential genes which creates a challenge when studying them since their complete knockdown...about other CRISPR methods for bacterial genome engineering   Learn about human CRISPRko, CRISPRi, and CRISPRa...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...particular protein DNA must first be transcribed into messenger RNA (mRNA). mRNA can then be translated at the... however, are expensive and very technically challenging to use. Alyssa D. Cecchetelli is a Scientist... expression in Escherichia coli: advances and challenges Production of recombinant proteins in plant root...

  18. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...motivates you!), know their unique skills and strengths (you will likely have reports with different levels.... Personally I found this to be particularly challenging. As PhD students and postdocs we are used to ...was a manager! Second, my direct report was not engaged because I only left him/her with small tasks and...

  19. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ... they will emit fluorescent light at similar wavelengths (See this handy emission spectra chart to find...the two of them because they emit at a similar wavelength. However, if your flow cytometer reads them out...Compensating between your fluorescent markers can be challenging! But we hope this guide can help you get started...

  20. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...kDa). This system also requires less genetic engineering than some of the other systems reviewed here....PROTAC) was developed for HaloTag. PROTACs are engineered molecules which target E3 ligases to specific...that it requires an additional component to be engineered or expressed beyond the tag and small molecule...
Showing: 281 - 300 of 676 results