...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...

... event – all while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help...all the words of support from scientists using Addgene in their labs. And it has been exciting to learn...McGovern Institute for Brain Research) – and an Addgene depositing scientist – stop by our booth to say...by booth #3431 if you’re at #SfN14 and meet our Addgenie crew and sign-up for a free plasmid! Also check...

...Conjugation, and Transfection Resources on Addgene.org Addgene's Synthetic Biology Collection...Here at Addgene, we often refer to plasmids as lab or experimental tools. They certainly are very handy...deposit the many plasmid tools available in the Addgene repository. To learn more about some other common...other Plasmids 101 posts, and keep an eye on the Addgene blog as scientists continue to explore Mother Nature.../07388550490904288 Additional Resources on the Addgene Blog Plasmids 101: Antibiotic Resistance Genes...

...corresponding portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with... community. Find the Zika Sensor Constructs at Addgene As we report, we have embedded this next generation...27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches...e15272. PubMed PMID: 27090089. Resources on the Addgene Blog Read other Synthetic Biology Posts Listen...Your Next Experiment Deposit Your Plasmids with Addgene ...Used to Study Dangerous Viruses Resources on Addgene.org Check out All the Plasmids from the Collin's...

...and tips Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols...interest.   While the VGS process isn’t new to Addgene, we are excited to share the procedures and Python....ymthe.2017.04.001 Additional resources on the Addgene blog Read all of our viral vectors related blog...

...out full plasmid sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered...annotation gaps in the plasmids available through the Addgene website. In addition, two initiatives are in the...University of Chicago. He was an early depositor at Addgene, and conceived SnapGene because he needed it for...post-launch iterations.     Additional Resources on the Addgene Blog Read about our SnapGene powered plasmid maps...feature database. Powering the plasmid maps on Addgene.org with SnapGene Server is useful because it helps...

... at Addgene.org Order Plasmids from Addgene! Find Free and Open Educational Resources at Addgene! ...microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast transformations... @ZebraElement     Additional Resources on the Addgene Blog Experimenting in an Open Source Lab: from...

...Viral Services Resources on the Addgene blog Viral Production at Addgene Viral Vectors 101: What is a Virus...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral...   Is there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available...

... al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods, 2019. PubMed PMID:...difficult. Find SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018. PubMed PMID...research and innovation. Find MerMAIDs plasmids at Addgene Opperman J et al., Nature Communications, 2019....plasmids at Addgene Kim et al., Nat Methods. 2019, PubMed PMID: 31235883. ... Various Addgenies...

...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog An “elegans” Approach...PubMed. Find plasmids from this publication at Addgene. Other strategies for CRISPR/Cas9 editing in ...PubMed. Find plasmids from this publication at Addgene. Scalable and versatile genome editing using ...

...Twitch calcium sensors, which are available at Addgene, can be used for ratiometric in vivo imaging. Griesbeck...the first of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx said. “For...(PubMed) Interested in More Hot Plasmids from Addgene? Check out our Hot Plasmid page, where we highlight...some of the popular & newly available plasmids at Addgene. If you're interested in plasmids for in vivo imaging...

...experiment? What if after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation...Suggestion Form.  Additional resources on the Addgene blog Browse blog posts from our Plasmids 101 blog...the various ways to quantify DNA Resources on Addgene.org Learn about our other beta projects Read our...

...Addgene is proud to present our updated CRISPR Guide! The CRISPR Guide helps scientists understand how...offer. Happy CRISPR-ing! More resources on the Addgene blog PRIME Editing CRISPR 101: Cas9 vs the Other...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...please let us know! Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate...terms of cost and content level. Sponsorship from Addgene and Harvard Integrated Life Sciences (HILS) made... do you share your science with the community? Addgene would love to hear about your efforts to engage...

... perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We do...Preparation of electrocompetent cells (protocol) At Addgene, we use the Mix & Go! E. coli Transformation Kit...large number of transformations being performed at Addgene, this adds up to a significant time savings. You...

...time on Addgene’s role in the rapid dissemination of these technologies, proposing that “Addgene, with ...has been a key driver of the technology.” As an Addgene vet of 12 years, I’m very familiar with the role...I discussed The State of Science with the five Addgene colleagues who read it with me, the consistent ...about many different things (part of the reason Addgene has been such a great fit for me!), the book was...Acknowledgements: I would like to thank fellow Addgenies Joanne Kamens, Marcy Patrick, Aliyah Weinstein...

...want to pick a topic and have no one show up. At Addgene, we wanted to think more closely about the wealth...: Discussing next-generation sequencing at the Addgene bioinformatics journal club! What happens during...messaging but we as scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (...most likely the primary drivers of frequency. At Addgene, we opted to start with one per month. When to ...own journal club! Additional resources on the Addgene blog Read our blog posts centered around scientific...

...suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche, Bernd, et ...27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver, Benjamin P., et al. "Genome-wide...27347757. Find plasmids from this publication at Addgene. 3. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker...26422227 Find plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa...nrmicro3569. PubMed PMID: 26411297 Resources on the Addgene Blog Catch Up On Your Cpf1 Background Read About...piggyBac Vectors in Genome Editing Resources on Addgene.org Check Out Our CRISPR Guide Pages Check Out MpAgo...