...developed the plasmids, pPSU1 and pPSU2. When restriction digested with PstI or EcoRV, these plasmids generate...available from Addgene. Learn How to Analyze a Restriction Digest References 1. Henrici, R. C., Pecen, ... Miniprep Protocol Learn How to Analyze Your Restriction Digests Make Your Agarose Gel Other Resources...

...covering the basics of science communication, tips and tricks for sharing your science with others, and introducing... sharing our educational resources and tips and tricks for those new to the lab, along with some behind-the-scenes...some much-deserved vacations, sunshine, and beach trips, of course.    *not me, though. I don't dance.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      ...

...provide resources to researchers in developing countries or that work in under-funded fields. Like Louis...among females in Ghana and in other developing countries” and “Almost all cervical cancers are caused by... our Cancer Pages Learn about Global Plasmid Distribution ...

...Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs Answered...Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick Will You Be My Mentor? Finding and Asking for...

...pace. To submit, simply click "Contribute Data."    Figure 6: Contributing data to the Data Hub is easy...in the Addgene antibody collection to use and contribute to the Antibody Data Hub.   More resources on...

...lab discovered that cellular mismatch repair contributed to a large number of unintended prime editing...streamline the process of prime editing, the Liu lab contributed an improved prime editing “architecture” that...alternate approach to RNA stability This recent contribution by the Adamson lab also addresses the problem...doi.org/10.1038/s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....

...both mouse and human cells are avaliable for distribution. To learn more about pooled library screening...signaling in cancer cells, deciphering the mechanism of matrix metalloproteinase-9 secretion, and identifying ...use. It’s just one of the many plasmids being distributed to and accelerating research in labs all around...

...are more than welcome in contributing to The Addictive Brain. A contribution can either be an article,...This post was contributed by guest blogger Chinmaya Sadangi, a postdoc at the University of Toronto. ...

... use the material in human subjects or clinical trials Acknowledgement in recipient’s future publications... benefits, we know that legal documents can get tricky. Here’s how Addgene helps: For Providers: One-time... FREE!) and we’ll take it from there. We will distribute your materials under the UBMTA terms, so you ...

...difficulty with NgAgo, but a few successes have trickled into the survey. In response to a bit of a Twitter...to make single base edits in DNA. The first, contributed by guest blogger Kristian Laursen from Cornell...mutated plasmid constructs. The second post was contributed by Addgene Scientist Mary Gearing and discusses...

...The following post was contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and...frameshift mutations. However these may require restriction fragment length polymorphism analysis, allele-specific... you to our guest bloggers! Daniel Bauer is a pediatric hematologist and principal investigator at Boston...

...Yeast. 1998 Jul;14(10):953-61. Note: Marcy Patrick contributed to the writing of this article....isolation of successful transformants. Addgene distributes several ready-to-use, modular plasmids, combining...

...amazing plasmid technologies contributed by our depositors, Addgene strives to make it easier for you to...among all the noise. Scientists have a variety of metrics they can use to evaluate the impact of their work...

...Recycle plastic packaging Packaging is a huge contributor to the increase in plastic manufacturing, accounting...accept and to print a free shipping label. Recycle nitrile gloves Terracycle is a company that takes items... a box with the prepaid label and fill it with nitrile gloves, affix the prepaid label, and divert these...

...NEBCutter2: For finding restriction enzyme cut sites Webcutter 2.0: For finding restriction enzyme cut sites ... different types of cloning methods, such as restriction enzyme cloning, Gibson assembly, or In-Fusion...

...heavy restrictions on what I could and couldn’t do as an intern. I expected that these restrictions would...choose what gene to focus on. The gene choice had restrictions. It couldn’t be a Covid gene, a toxin, or an...

... a repair template, using Gibson Assembly or restriction enzyme cloning. Streptomyces bacteria are more... presence of just a single organism, such as Clostridium difficile, could be targeted and preferentially...base editing in Streptomyces Some of the most industrially and clinically relevant bacterial species reside...CM, Ousterout DG (2020) In Vivo Targeting of Clostridioides difficile Using Phage-Delivered CRISPR-Cas3...

... This post was contributed by Magdalena Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. ...general, I think that this is an (e.g. great contribution to the community because of..." This first couple...on-line repository (like GitHub, Zenodo, Figshare, Triad) while the wet-lab resources, like constructs or...

... such as Stbl3s. ITR loss can be detected by restriction digest using enzymes, like SmaI, which have recognition...organism or tissue type-specific, which can help you restrict the expression of your transgene to a specific...result is a permanently flipped transgene. By restricting the availability of Cre, lox sites lets researchers...

...Minigenomes - A Safe Way to Study Dangerous Viruses Contributed by guest blogger Tessa Cressy from the labs of...leaving virulent components behind. Minigenomes are stripped down viral genomes containing only the sequences...fantastic depositors and guest writers. Your contributions make Addgene and the Addgene Blog useful resources...