...used rAAV FLEx-based vectors to study the neural circuitry behind the mechanisms of sleep and anesthesia....Cre recombinase protein." Journal of Biological Chemistry 259.3 (1984): 1509-1514. PubMed PMID: 6319400....behaviors: a critical analysis." Pharmacology Biochemistry and Behavior 122 (2014): 144-157. PubMed PMID... and approaches for optogenetics." Biological psychiatry 71.12 (2012): 1033-1038. PubMed PMID: 22480664...

...a desired component are cloned into Golden Gate entry vectors via traditional restriction enzyme cloning...cloning, or annealing of oligonucleotides. These entry vectors are used in a Golden Gate cloning step to...to generate the final construct. Up to eight entry vectors can be used for each Multisite Gateway compatible... Production group at Max-Planck Institute of Biochemistry has developed a number of expression vectors..., and plant resistance cassette) in pUC19 based entry vectors, as well as the pGreen-IIS based destination...

...genetic circuitry to the downstream output. Tuesday morning's talks were also about circuitry and synthetic...shuttle, our driver regaled us with tales of back country skiing, fly fishing, and local grizzly bear attacks...

... science began in high school. “I had a great chemistry teacher in 10th grade and then fell in love with...do,” Stegmeier says He began this journey in biochemistry made the foray into genetics during graduate... efforts in gene therapy either through direct industry collaborations, grants, or setting up commercial...

... like serotype, promoter, or tool type and each entry contains an image along with key experimental details...to input your data for sharing on the site. The entry form has 5 general question types and it takes approximately...use GCaMP expression? Direct Fluorescence? Immunohistochemistry? Did you use any relevant animal treatment...

...student hasn’t been asked the question: “Academia or industry?” Once academia was the clear answer with a well-defined...uses it to match qualified scientists to jobs in industry, academia, and government. This great resource... more information Follow people in academia, industry, and government on social media (Twitter, Facebook...

...diverse array of processes. The food processing industry, for instance, employs psychrophilic enzymes for...extremely useful benefits for any food processing industry as it can potentially help minimize the incidental...iGEM teams to interact with personnel from the industry and brainstorm with each other. The event also...

...applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western blotting...

...industries tend to hire their former interns to fill entry-level positions right out of college. Prior to March...also serve you well when you are applying for an entry-level position following graduation. While these...for internships, scholarships, fellowships, and entry-level positions post graduation. Many thanks to...

...unique regions as well, though. Viral packaging and entry The genetic cargo requires viral packaging to infiltrate... from the transfer plasmid. Viral packaging and entry The viral packaging proteins for AAV are rep and...replication of the dsDNA virus. Viral packaging and entry Adenovirus is quite large and has more helper plasmid...

...resulting in faint signals (Schwab et al., 1979; Buttry & Goshgarian, 2015). Nevertheless, the use of these...visualizing motor, visual, and auditory neuronal circuitry in vivo. A variant of RABV has been developed ... allowed for detailed mapping of the neuronal circuitry involved in learning and memory (Kohara et al....PMID: 21825165. PubMed Central PMCID: PMC3174680. Buttry, J.L., and Goshgarian, H.G. (2015). WGA-Alexa Transsynaptic...

...visualization of specific proteins in situ immunocytochemistry is perhaps the most common approach. With...students at the Max Planck Institute for Biophysical Chemistry, Göttingen in Germany. I attended this symposium... labeled proteins can be detected using click-chemistry that covalently links the azido containing AHA...

...needs to be cloned into an entry vector. The 2in1 cloning system contains two entry vectors (pUC57-L1L4 and...NC, CN, and CC). The 4 kits also contain the two entry vectors pUC-L1L4 and pUC-L3L2. Find the 2in1 plasmid...

... of transitioning a scientist from academia to industry, you might want to consult the Cheeky Scientist...We Turn Academics into Confident and Successful Industry Professionals”. This larger shop might appeal ...excellent in coaching for communication skills. Industry transition is also an area of specialty for Michelle...

...slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team from China’... (i.e. RNA replication with no proteins). The chemistry is complex and this work requires an interdisciplinary...forms as we shared millions of years of common ancestry with worms before evolutionary divergence. Nikolaus...

...challenging to compete in today’s tough academic or industry job market for biology PhD’s. I was facing the...in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University of Manchester as...receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in...

...can be easily modified in order to facilitate its entry into the target cells (Beatty et al., 2012). These...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...therapy: Current state and clinical perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/...

...machine learning to solve difficult problems in chemistry and chemical biology.       David R. Liu is Director... Faculty at the Broad Institute; Professor of Chemistry and Chemical Biology at Harvard University; and...Institute Investigator. Liu’s research integrates chemistry and evolution to illuminate biology and enable...

...generates pores in the cell membrane, allowing for entry of the RNP into the cytoplasm. In addition to the...forms pores in the nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated...electroporation of zygotes." Journal of Biological Chemistry (2016): jbc-M116. PubMed PMID: 27151215. PubMed...

...those conversations, reading online healthcare industry publications like FiercePharma, FierceBiotech,...about the trends and workings of the healthcare industry. Finally, on-campus resources like career offices... both provide up-to-date information about the industry, recruiting processes, and can connect you to ...