...many career pathways exist for scientists and engineers, a few brave scientists will choose to start their... interdisciplinary, with emphasis on genetic engineering, sequencing, and translation of results to companies....” Take home message Starting a lab can be a challenging process. Each PI emphasizes the importance of...

...Non-Continuous Evolution (PANCE) selection systems to engineer better and faster ABEs (Fig. 2). These systems...ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors? Targeted evolution ...Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR (2020) Phage-assisted...

...this virus. While various Cas9 mutants have been engineered to perform unique editing tasks, classic Cas9...LentiCas9-blast is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al...makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity also causes growth rate variability...

...fluorescent as well as FRET-based biosensors have been engineered to detect a wide array of neurotransmitters ranging...biosensors? Well, a lot of these sensors are already engineered into viral vectors, so if you want to use them...Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience...

...pGGA008 (AlcA) and pGGC011 (AlcR) from red flame GreenGate kit Steroid-regulated LexA promoter XVE (synthetic...shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light... induce transcription of your target gene? The strength of inducible promoters can vary a lot. If you’...

...and tractable tools for genome engineering. Scientists have engineered viruses to target specific cell...infect. In the lab, this means viruses can be engineered to be cell type specific. Once inside the host...

...reacting to a chemical, but by emitting a specific wavelength of light in response to excitation by a laser... degrading them. You’ll also need to know the wavelength of laser needed to excite them - and it’s a good...   Fig 2: Try to inversely pair fluorophore strength with protein abundance.    Second, you’ll ...

...Immunologists are coming together to tackle the challenge of curing cancer in cross-disciplinary projects...CRISPR/Cas9 methodology with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor..., CRISPR/Cas9 genome editing allows targeted engineering of the TCR locus. He also highlighted the UCSF...

... on differences in emission wavelength. Filtering the emission wavelength partially separates the signals...In fluorescent imaging, you use the excitation wavelength to specify which FP emission you’ll capture. ...

...computation work comes with new sets of challenges. One challenge with computational analyses is to keep... are doing what you are doing. Another common challenge is to keep track of what works, and what does ...

...advantage to using the CRISPR/Cas system for genome engineering is the fact that Cas9 can be easily programmed...smegmatis (Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair...UNC Chapel Hill.   References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM (2009) Limiting the Persistence...

... is a postdoc at Harvard University where she engineers genetically encoded voltage indicators. Tian developed...mutant sensors expressed in a mammalian cell line engineered to be electrically excitable. The mutants are.... Tian notes that this platform can be used to engineer many types of biosensors.   This screening method...

...injected with an immunogen. This might be the full-length protein that you want to generate antibodies against...Otherwise, you might experience variability in the strength of staining and background noise. If you do use...control across experiments to be aware of signal strength. In general, polyclonal antibodies produce a higher...

... evaluate degradation of ENL. Cell lines were engineered to express ENL-FKBP12F36V and endogenous ENL ...evaluate degradation of MELK. Cell lines were engineered to express FKBP12F36V-MELK and endogenous MELK... evaluate degradation of YY1. Cell lines were engineered to express YY1-FKBP12F36V using CRISPR/Cas9-mediated...

...excitation wavelength. A fluorophore is less likely to bleach when it is excited off the peak wavelength, since... excitation powers are often used to reduce the length of the experiments. As the photobleaching rate ...

...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. ...Consulting works remotely, will travel for speaking engagements and has many areas of specialty from leadership...but the website looks interesting and it is in English and Spanish. She is based in Spain. The bottom ...

...particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...literature! PubMed PubMed is the classic search engine for finding papers. It’s been around since 1996...Zuckerberg Initiative. Meta is not a traditional search engine. Instead, you create your own research feed by ...

...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability ...tool to the CRISPR tool box.  Nanoblades are engineered murine leukemia virus (MLV)-like particles loaded...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades...

...high-complexity reactions? The researchers over at New England Biolabs® thought otherwise! They suspected a data-driven... Golden Gate Assemblies. Image courtesy of New England Biolabs.   DAD Tools The three DAD tools used...Temperature Incubation time/cycling Overhang length T4 DNA ligase 25 °C 1 hour 3-base or ...

...its ground state by emission of a photon with wavelength specific to the acceptor In order for FRET to...quantify mechanical tension (a molecular “spring”) (Meng et al., 2008), and monitor neuronal activity (voltage...Nature 388.6645 (1997): 882. PubMed PMID: 9278050. 3. Meng, Fanjie, et al. "A fluorescence energy transfer‐...