...student at Johns Hopkins University, where he is trying turn a few exoplanets into a successful PhD thesis...ensued as “alternative facts” became a part of our country’s rhetoric and drastic cuts to the NIH budget were...

...tag insertion, you should test a new crRNA and/or try out an alternative tag (3xFLAG, Myc, V5, or HA). ...pull-down assays include immunoprecipitation mass spectrometry (IP/MS) and chromatin immunoprecipitation (ChIP... protein complexes are then analysed by mass spectrometry to identify the interacting proteins. Similarly...analyzed for tagging efficiency using standard immunocytochemistry (ICC) assays.  Figure 1: Three ingredients...single-stranded donor DNA (V5 tag) were quantified by immunocytochemistry assays. Dewari et al, eLife 2018;7:e35069...

...disease-inducing genetic mutation in a patient, trying to transdifferentiate any of the patient’s cells...

...this pathway is further downregulated. Rather than try to improve HDR, scientists have developed two classes...

...will introduce unwanted variation into your data. Try different types of pipettes and pipetting methods...application correctly is imperative. Picking your chemistry  The two most common qPCR chemistries are hydrolysis...primer/probe actions to a single step. Scorpion chemistry in particular has been reported to work well in...features of several commonly used chemistries)   Chemistry Background noise Probe Allelic specificity...to need to adjust amounts of reagents in your chemistry, unless running a multiplex reaction or on a rapid...cycle machine. Melt Curves An advantage of Sybr chemistry is the ability to run a melt curve to validate...multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to five or six reactions in...

...activation domain is an incredible tool for researchers trying to activate target genes, the relatively large ...to customize for targeting your gene of interest. Try them today! Ordon J, et al. Plant J. 2016. PubMed...food and beverage industry, the bioethanol industry, and the pharmaceutical industry, depend on the yeast... pull-down methods and identified using mass spectrometry. The Roux Lab’s new and improved version of ...were validated by both Western blotting and immunohistochemistry, and are summarized in the table below: ...

...other inntags available through Addgene so you can try these incredible new tools yourself! The tags can...their wild-type counterparts - a real boon to those trying to understand physiological interactions and functions...set of 12 promoter-less Gateway® entry clones to be used with other entry clones encoding the ORFs of interest... article through Addgene. Nihongaki et al., Chemistry and Biology. 2015 Feb 19; 22(2):169-74. High...fusion proteins from any ORF available as a standard entry clone. ORFs from an ORFeome Gateway library or any...traditional restriction enzyme cloning to create an Entry vector containing a promoter for constitutive (CMV...expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9, EGFP, mCherry, iRFP, tdTomato...

...indicating the breakdown of ancestry associated dependencies across ancestry groups (right).    Seeking...divided into those of African ancestry(orange) and those of other ancestry groups (grey). Misek and... representation of every ancestry group, they should have found more ancestry-associated dependencies ...any other ancestry group. With the existing methodology, they estimate that the African ancestry-associated...Institute, where an exploration into the effects of ancestry and germline sequence variation in cancer cell...down unexpected paths. Though this research uses ancestry groups rather than self-reported race, it highlights...screens in DepMap to see if they could identify the ancestry of the cell lines (European, East Asian, Indigenous...

... to travel meant the accessibility varied country to country.   Figure 1: Number of countries Addgene ...Views from a country are represented in blue; darker blue indicates more views from that country.   While ...Views from a country are represented in blue; darker blue indicates more views from that country. Stats and...the USA, but our materials are available to any country that we can export to — so far, we’ve shipped materials...distributors Distributor Partnership Start Date Country Served Beijing Zhongyuan 2012 China ...distributors broadly helped increase access in the country, and we can see a growth in material distribution...distributor helped solve an issue specific to that country. ‌For example, when we began offering temperature-sensitive...

...transferred to an industry position. If you cannot get an Industry H-1B, remember that industry career paths...Subject Industry H1B Academic H1B National Interest Waiver EB-1A EB-1B Cap subject industry H-1B There...available to you including careers in academia, industry, non-profit, and government sectors.  Within these...area, then the research role could be the point of entry into that field. In this post, we discuss the following...endeavor including but not limited to biotechnology, chemistry, architecture, engineering, mathematics, physical...-international focus. Candidates in need of an industry H-1B need to be aware of the April 1 deadline ...start working in October of the same year. The Industry H-1B visa is not the same as an academic H-1B ...

...hybridization histochemistry: use for mRNA detection.” The Journal of Histochemistry and Cytochemistry : Official...interaction has also been adapted for use in immunohistochemistry and antibody labelling. In these experiments...referred to as the Avidin-Biotin Complex (ABC) immunohistochemistry (IHC). For more information on ABC and IHC...labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In ...Attachment to Proteins; Symposium: Nutrition, Biochemistry and Molecular Biology of Biotin” J. Nutr, 129...Official Journal of the Histochemistry Society, 41.12 (1993): 1751–1754.PubMed PMID: 8245422. Additional ...

...study protein-protein interactions. Flow cytometry Flow cytometry is used to detect proteins on the surface...used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could be cells or ...investigate in each panel. An advantage of flow cytometry is the ability to use multiple fluorescent signals...compare between samples. Learn more about flow cytometry. Cell and tissue labeling Antibodies can be used...within the cell or tissue as well. Similar to flow cytometry, you’ll use antibodies to stain for markers of...can be analyzed using techniques such as flow cytometry. Conclusion Whew! Now you know that antibodies...

...shared his flow cytometry expertise in three posts. An Introduction to Gating in Flow Cytometry, Designing...shared her flow cytometry expertise in two posts. Flow Compensation and Flow Cytometry Controls Priyamvada... guide to understanding your flow data. Flow Cytometry Readouts Toj, a summer intern who shared their...validation expertise. Antibody Validation for Flow Cytometry Rob Hurt, whose expertise and suggestions were...

...schools to promote chemistry degrees and provide advice to students who want to study chemistry. The society... scientists are introduced to the biology and chemistry societies that will form the core of their scientific...with the goal of facilitating networking between chemistry students, especially those studying to earn a ...events throughout the year, including a Christmas Chemistry symposium and a career night. The society also...students in society and to support iGEM alumni-industry networking to promote the creation of start ups...

...while flow cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry...signaling molecule has a weak signal. For flow cytometry, secondary antibodies may be indicated on occasion...assumption is incorrect. With the exception of flow cytometry, most immunoimaging assays do not allow for absolute...the need for a secondary antibody. Other immunohistochemistry advances have found ways to increase signals...

...However, internationalization, the ability to cross country boundaries for both professional and personal development...boundaries and should not be confined by birth country or financial means. Instead, talent should be realized...talented professionals (who migrated from their country some years ago looking for their career improvement...bright students that will be the future of the country. Brain drain is a serious problem in several countries...immediate frontiers. Moreover, sharing a culture/country of origin with an international mentor creates ...University of Navarra, and the University of the Basque Country. Mentees represented a diverse group of individuals...

...and even some you’ve likely never heard of. Each entry displays the following information for a Cas enzyme...display both the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently... 2 – List of resources and properties for a Cas entry (SpyCas9a) from CasPEDIA.   How can I use it...RefSeq ID, etc.) and you will see a table of protein entry results. For the advanced search options, this is...System for Class 2 CRISPR-Cas Enzymes [Preprint]. Biochemistry, Biophysics, and Structural Biology. https:/...

...the most involvement with Step 1, and, while data entry can be a chore, we’ve made it easy to send us your...information in a deposit spreadsheet. The data entry process On the Addgene home page, if you scroll ...top of the page, you’ll see two options for data entry: “Submit Online Deposit” and “Download Deposit Spreadsheet...blog post for a thorough guide to our online data entry process. Figure 1: Begin the deposit process by...deposit spreadsheet will help expedite your data entry and make it easier than ever to share your discoveries...

...an important role in viral entry, which are inhibitors of SARS-CoV-2 entry and become upregulated during...plasmids, with many available to scientists in industry. Since March 2020, we have received over 13,000... which human genes are important for SARS-CoV-2 entry into human lung epithelial cells (Biering et al....glad to join these efforts and help academic and industry scientists through plasmid sharing and more. As...

...create plasmids for fluorescence imaging or flow cytometry applications in mammalian cells. The modules used... various constructs with applications in flow cytometry approaches in mammalian cell culture systems (...deoxyribonucleic acid. The Journal of biological chemistry. 1961. March;236:864–875. Pubmed PMID: 13790780...Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. PLoS One. 2015...deoxyribonucleic acids. The Journal of biological chemistry. 1962. June;237:1961–1967. Pubmed PMID: 13918810...