Deep Dive: qPCR
Type
Blog Post
Published
Sept. 29, 2022, 1:15 p.m.
...will introduce unwanted variation into your data. Try different types of pipettes and pipetting methods...application correctly is imperative.
Picking your chemistry
The two most common qPCR chemistries are hydrolysis...primer/probe actions to a single step. Scorpion chemistry in particular has been reported to work well in...features of several commonly used chemistries)
Chemistry
Background noise
Probe
Allelic specificity...to need to adjust amounts of reagents in your chemistry, unless running a multiplex reaction or on a rapid...cycle machine.
Melt Curves
An advantage of Sybr chemistry is the ability to run a melt curve to validate...multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to five or six reactions in...