We narrowed to 259 results for: try

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Nov. 3, 2022, 12:15 p.m.

Open science resources like Addgene's Data Hub increase reproducibility, reduce validation and optimization timeframes, and drive science forward.

Sept. 6, 2022, 1:15 p.m.

Learn about how to identify and develop creativity in the lab - a skill that's valuable in and out of the sciences!

Jan. 18, 2022, 2:15 p.m.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

April 13, 2021, 1:15 p.m.

Discover the history of Rett Syndrome research, the role of the MECP2 gene, and some of the latest breakthrough therapeutic approaches for Rett Syndrome.

Nov. 19, 2020, 2:15 p.m.

Learn why codon usage bias exists and techniques you can use to avoid the detrimental affects of codon usage bias on heterologous protein expression - codon optimization and expression of alternative tRNAs.

Sept. 27, 2018, 1:09 p.m.

Get time management tips form blog editor and Outreach Scientist Tyler J. Ford as part of our series on transferable skills.

Dec. 5, 2017, 1:52 p.m.

Learn how we titer AAV for our viral service at Addgene and find tips for improving your AAV tittering methods.

Nov. 15, 2017, 3 p.m.

Learn why it's a good idea to share your science through video and check out the new research video sharing platform We Share Science.

Nov. 3, 2016, 2:30 p.m.

Joanne Kamens and Tyler Ford discuss the benefits of using Reddit to communicate your science.

Oct. 20, 2016, 2:30 p.m.

Find 10 tips on how to give the perfect science talk

Aug. 23, 2016, 2:30 p.m.

Use site directed mutagenesis to insert small mutations into your plasmid of interest and follow these tips for a smooth process with easy validation.

Aug. 2, 2016, 2:30 p.m.

The next post in the Plasmids 101 series focuses on the promoter region, the element responsible for initiating the transcription of your insert into RNA.

April 3, 2014, 8:05 p.m.

This post describes the biology and use of integrase-deficient lentiviral vectors in comparison to standard lentiviral vectors.

May 13, 2025, 1:15 p.m.

Review of off-target effects in CRISPR experiments. Topics covered include: measuring, preventing, and controlling for off-target events.

Feb. 8, 2024, 2:15 p.m.

Learn how to incorporate phosphoserine into your protein of interest to study the effects of phosphorylation.

June 23, 2016, 2:30 p.m.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.

Learn more about NanoBiT technology and explore examples of HiBiT-tagged fusion proteins in action.

Oct. 2, 2025, 1:15 p.m.

Thinking about a career in science policy? In this article, we cover the who, what, when, where, why, and how of science policy careers.

Sept. 22, 2020, 1:15 p.m.

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

June 24, 2020, 5:45 p.m.