We narrowed to 468 results for: try

...position and directionality of the sequence you are trying to clone. Figure 2: MoClo assemblies depend...

...hard to conceptualize where the populations are, try drawing a quadrant over the plot that divides it ...data in the literature. Data reported from flow cytometry experiments can be a little challenging to understand... on how to read a flow plot.  Dot plots Flow cytometry is a method that allows users to analyze single...Created with biorender.com.   The goal of flow cytometry is to use increasingly more stringent markers ...in the same order. However, in an actual flow cytometry run, all markers are read in parallel.  Let’s ...more things to learn about when diving into flow cytometry data. There’s different ways to use FSC and SSC...resources on the Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies...

...different tasks. I use google calendar for this and try to give myself flexibility by never placing too many...

...Okay, let’s play. I’ll list the examples and you try to guess the category: ELISA...qPCR...Digital droplet... quantitation of DNA copy number." Analytical chemistry 83.22 (2011): 8604-8610.4. Hindson CM, Chevillet...

...question of how we can support researchers as they try to keep up with the growing amount of interdisciplinary...

...Volunteer moderators set the rules for these forums and try to keep discussions on-topic and, at least sometimes...

... know a lot about and that you care about. Don’t try to present topics that are unfamiliar to you because...Joanne's 10 Things Scientists Ask about Finding an Industry Job Talk What’s Your Story? By Herminia Ibarra...

...directed mutagenesis. In addition, you may want to try different concentrations of template (e.g. 0.1-1.0...in the polymerase chain reaction." Analytical Biochemistry 209.2 (1993): 284-290. PubMed PMID: 8470801....

...this and thinking “Gee, I’d love to give these a try myself,” look no further! You can package your own...virus G protein (VSV-G), which facilitates viral entry into the host cell.  Transfecting these three plasmids...

...have a significant impact on your data. You are trying to generate a knockout cell line, with the goal...

...phosphorylation affects protein function, researchers should try to reverse the effects of pSer-incorporation on enzyme... model systems coupled with advances in mass spectrometry suggest that phosphorylation is far more prevalent...suppression, as described (6). To determine the stoichiometry of phospho-incorporated to non-phospho-incorporated... pSer incorporation can also done using mass spectrometry to identify a mass shift proportional to a single...

...synthetic biology, you should consider giving it a try! References: 1. Gibson DG, Young L, Chuang RY, ...

... issue. This may be more challenging if you are trying to apply for an outright job in a field where you...organizations, universities, professional societies, and industry through convening activities, formal lobbying,...enter this career trajectory. One common route of entry is through a science policy fellowship program which...and local governments, professional societies, industry, and beyond!  Learn and develop valuable science...

...type- and context-dependent, it may make sense to try multiple CRISPR tools when setting up your experimental...

...support, and distribution capabilities. Give HiBiT a try and see your favorite proteins in a whole new light... green fluorescent protein. Annual Review of Biochemistry, 67, 509–544. https://doi.org/10.1146/annurev.biochem...

...insertion site. If you are using the ivTRT method, try to make the first two bases of the left homology ...

...if you’re working with plants, don’t be afraid to try your hand at CRISPR. References Belhaj K, Chaparro-Garcia...

...Nelson et al., and Tabebordbar et al. each chose to try exon skipping in DMD mice, which have a mutation ...

...system. Table 3: AAV tissue tropism. To target... Try using... CNS AAV1, AAV2, AAV2-retro (AAVrg), AAV5... for gene transfer. The Journal of Biological Chemistry, 276(23), 20610–20616. https://doi.org/10.1074...

...or if no monomers exist in your sample, you could try linearizing the plasmid via restriction digestion...colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20. doi: 10.1021/bi00850a028...19233255; PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid...