... new to the mARG expression are advised to first try to produce GVs in their own lab using the most reliable...is easy: simply grow cells in Lennox LB (10 g/L tryptone, 5 g/L yeast extract, and 5 g/L NaCl) or on LB-agar...Magnetic Imaging and Control of Cellular Function. Biochemistry 56, 5202–5209 (2017). Bourdeau, R. W. et al....

...what the ploidy of your target site is. If you are trying to introduce a homozygous mutation, it’s helpful...

...this and are still struggling with idea generation, try this thought exercise: Consider the issue as if there...

...insertion site. If you are using the ivTRT method, try to make the first two bases of the left homology ...

...if you’re working with plants, don’t be afraid to try your hand at CRISPR. References Belhaj K, Chaparro-Garcia...

...phosphorylation affects protein function, researchers should try to reverse the effects of pSer-incorporation on enzyme... model systems coupled with advances in mass spectrometry suggest that phosphorylation is far more prevalent...suppression, as described (6). To determine the stoichiometry of phospho-incorporated to non-phospho-incorporated... pSer incorporation can also done using mass spectrometry to identify a mass shift proportional to a single...

...type- and context-dependent, it may make sense to try multiple CRISPR tools when setting up your experimental...

...have a significant impact on your data. You are trying to generate a knockout cell line, with the goal...

...synthetic biology, you should consider giving it a try! References: 1. Gibson DG, Young L, Chuang RY, ...

...Nelson et al., and Tabebordbar et al. each chose to try exon skipping in DMD mice, which have a mutation ...

... issue. This may be more challenging if you are trying to apply for an outright job in a field where you...organizations, universities, professional societies, and industry through convening activities, formal lobbying,...enter this career trajectory. One common route of entry is through a science policy fellowship program which...and local governments, professional societies, industry, and beyond!  Learn and develop valuable science...

...expression in a variety of cell types, but you should try to find the best AAV for your particular experiment...: impact of larger genomes on infectivity and postentry steps." Journal of virology 79.15 (2005): 9933...

...collaboration with Prof. Alexis Komor and together they are trying to broaden our understanding of the base editing...

...spread from the injection site it is advisable to try a few different injection titers and volumes to determine...modifications, and other factors that AAV utilizes to gain entry into the cell (Dudek, AM. 2018).  If the serotype...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. J Virol 92. https:/...

...or if no monomers exist in your sample, you could try linearizing the plasmid via restriction digestion...colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20. doi: 10.1021/bi00850a028...19233255; PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid...

...student at Johns Hopkins University, where he is trying turn a few exoplanets into a successful PhD thesis...ensued as “alternative facts” became a part of our country’s rhetoric and drastic cuts to the NIH budget were...

...new-and-improved fluorescent protein you want to try, just swap in a new fluorescent ligand and see the...its substrate. Then, all it takes is a bit of chemistry magic to produce a wide range of useful molecules...incorporate an alkene to be labeled through click chemistry. Although these multi-component systems can be...brings up bad memories of undergraduate organic chemistry lab, don’t worry – many SNAP-tag and HaloTag ligands...Tag for Multiprotein Labeling in Living Cells. Chemistry & Biology, 15(2), 128–136. https://doi.org/10.1016...L. (2016, September 8). Better Dyeing Through Chemistry & Small Molecule Fluorophores. Addgene Blog. https...https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores Los, G. V., Encell...

...tag insertion, you should test a new crRNA and/or try out an alternative tag (3xFLAG, Myc, V5, or HA). ...pull-down assays include immunoprecipitation mass spectrometry (IP/MS) and chromatin immunoprecipitation (ChIP... protein complexes are then analysed by mass spectrometry to identify the interacting proteins. Similarly...analyzed for tagging efficiency using standard immunocytochemistry (ICC) assays.  Figure 1: Three ingredients...single-stranded donor DNA (V5 tag) were quantified by immunocytochemistry assays. Dewari et al, eLife 2018;7:e35069...

...this pathway is further downregulated. Rather than try to improve HDR, scientists have developed two classes...

...disease-inducing genetic mutation in a patient, trying to transdifferentiate any of the patient’s cells...