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We narrowed to 469 results for: try

Showing: 221 - 240 of 469 results

  2. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ... know a lot about and that you care about. Don’t try to present topics that are unfamiliar to you because...Joanne's 10 Things Scientists Ask about Finding an Industry Job Talk What’s Your Story? By Herminia Ibarra...

  3. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...directed mutagenesis. In addition, you may want to try different concentrations of template (e.g. 0.1-1.0...in the polymerase chain reaction." Analytical Biochemistry 209.2 (1993): 284-290. PubMed PMID: 8470801....

  6. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...Okay, let’s play. I’ll list the examples and you try to guess the category: ELISA...qPCR...Digital droplet... quantitation of DNA copy number." Analytical chemistry 83.22 (2011): 8604-8610.4. Hindson CM, Chevillet...

  7. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ... new to the mARG expression are advised to first try to produce GVs in their own lab using the most reliable...is easy: simply grow cells in Lennox LB (10 g/L tryptone, 5 g/L yeast extract, and 5 g/L NaCl) or on LB-agar...Magnetic Imaging and Control of Cellular Function. Biochemistry 56, 5202–5209 (2017). Bourdeau, R. W. et al....

  8. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...what the ploidy of your target site is. If you are trying to introduce a homozygous mutation, it’s helpful...

  10. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...have a significant impact on your data. You are trying to generate a knockout cell line, with the goal...

  11. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...phosphorylation affects protein function, researchers should try to reverse the effects of pSer-incorporation on enzyme... model systems coupled with advances in mass spectrometry suggest that phosphorylation is far more prevalent...suppression, as described (6). To determine the stoichiometry of phospho-incorporated to non-phospho-incorporated... pSer incorporation can also done using mass spectrometry to identify a mass shift proportional to a single...

  13. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...this and thinking “Gee, I’d love to give these a try myself,” look no further! You can package your own...virus G protein (VSV-G), which facilitates viral entry into the host cell.  Transfecting these three plasmids...

  15. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...support, and distribution capabilities. Give HiBiT a try and see your favorite proteins in a whole new light... green fluorescent protein. Annual Review of Biochemistry, 67, 509–544. https://doi.org/10.1146/annurev.biochem...

  16. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ... issue. This may be more challenging if you are trying to apply for an outright job in a field where you...organizations, universities, professional societies, and industry through convening activities, formal lobbying,...enter this career trajectory. One common route of entry is through a science policy fellowship program which...and local governments, professional societies, industry, and beyond!  Learn and develop valuable science...

  20. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...spread from the injection site it is advisable to try a few different injection titers and volumes to determine...modifications, and other factors that AAV utilizes to gain entry into the cell (Dudek, AM. 2018).  If the serotype...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. J Virol 92. https:/...
Showing: 221 - 240 of 469 results